RESUMEN
The aim of this study was to evaluate the erosion-inhibiting effect of two toothpastes on the development of erosion-like lesions, by a confocal laser scanning microscope (CLSM). Forty human enamel blocks were divided into five groups (n = 8), in accordance to evaluate the GC MI Paste Plus and Oral B with stannous fluoride, applied as slurries and associated with toothbrush. Specimens were submitted to an erosion challenge from citric acid (0.5%, pH = 2.8), for 5 min, six times a day, alternating in artificial saliva immersions. Reference group was not exposed to treatment. Part of specimens (Groups 02 and 03) was exposed twice daily just to slurries, for 2 min, therefore specimens from Groups 04 and 05 were also abraded, for 30 s. The enamel surfaces were morphological characterized using CLSM images, with mineral loss being measured using the resulting 3D images referenced to an un-challenged portion of the sample. Step values were compared using the one-way ANOVA test. CLSM was shown to be a viable, noncontact, and simple technique to characterize eroded surfaces. The statistical difference in the step size was significant between the groups (P = 0.001) and using multiple comparisons a statistically significant protective effect of toothpastes was shown when these were applied as slurries. Although groups submitted to tooth brush showed mineral loss similar to reference control group, due to the damages of abrasion associated.
Asunto(s)
Esmalte Dental/ultraestructura , Erosión de los Dientes/prevención & control , Humanos , Microscopía Confocal , Erosión de los Dientes/patología , Cepillado Dental/efectos adversos , Pastas de Dientes/efectos adversosRESUMEN
We aim to characterize natural caries enamel lesions by fluorescence spectroscopy. Sixty human samples with natural noncavitated caries lesions on smooth surfaces were selected and classified into three groups: dull, shiny, and brown lesions. All the samples were analyzed externally at the natural surface and after hemisectionig internally at the center of the lesion. The lesions were excited with a 405-nm InGaN diode laser and the fluorescence was collected with a single grating spectrometer. Four emission bands (455, 500, 582, and 622 nm) are identified in both sound and carious regions. The area under each emission band is correlated with the total area of the four bands for the sound and carious regions. The detected fluorescence from natural and cut surfaces through the caries lesions is not statistically different for the shiny and dull lesion, but is different [analysis of variance (ANOVA) (p<0.05)] for brown lesion at all emission bands. At the 405-nm excitation wavelength, the area of the fluorescence bands at 455 and 500 nm differ statistically for natural carious lesions and sound tissue.
Asunto(s)
Caries Dental/diagnóstico , Espectrometría de Fluorescencia/métodos , Caries Dental/metabolismo , Caries Dental/patología , Esmalte Dental/anatomía & histología , Esmalte Dental/química , Fluorescencia , Humanos , Rayos LáserRESUMEN
OBJECTIVE: To report the use of spectroscopic analysis of dental fluorescence excited with a blue InGaN laser diode operating at 405 nm. METHOD: The spectra resulting from three classifications of smooth surface non-cavitated caries lesions (dull, shiny, brown) with 20 samples in each group were examined using the ratio of integrated fluorescence intensity in two spectral bands. RESULTS: All lesions demonstrated spectra which were significantly different from sound tooth structure. As expected, the 'brown' lesions demonstrated a significantly different spectral profile from the two white spot lesion classifications. Dull and shiny lesions had significantly different spectral measurements when examining the ratio of the integrated fluorescence in spectral bands between 480-500 and 620-640 nm. CONCLUSION: This method has application for detection of dental caries as well as demonstrating potential application to evaluate lesions which may represent different degrees of caries activity.