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Can J Microbiol ; 58(1): 54-66, 2012 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-22182199

RESUMEN

The diversity and antimicrobial activity of endophytic fungi associated with the Brazilian medicinal plant Solanum cernuum Vell. were studied during summer and winter seasons. A total of 246 fungal isolates were obtained, including 225 filamentous fungi and 21 yeasts. They were identified by morphological, physiological, and molecular methods. Fifty-five different taxa represented by the phyla Ascomycota (33 taxa), Basidiomycota (21 taxa), and Zygomycota (one taxon) were identified. The most abundant taxa were closely related to Arthrobotrys foliicola , Colletotrichum gloeosporioides , Coprinellus radians , Glomerella acutata , Diatrypella frostii , Phoma glomerata , Mucor sp., Phlebia subserialis , Phoma moricola , Phanerochaete sordida , and Colletotrichum sp. A total of 265 fungal extracts were screened and 64 (26.01%) displayed antimicrobial activities. Among these extracts, 18 (28.12%) presented antibacterial and antifungal activities, 42 (65.62%) displayed selective antibacterial activity, and four (6.25%) exhibited only antifungal activity. The best values of minimum inhibitory concentration were obtained from extracts of Cryptococcus rajasthanensis , Glomerella acutata, Leptosphaeria sp., and Phoma glomerata ranging from 7.8 to 15.62 µg/mL. This study is the first survey of the endophytic fungi community associated with S. cernuum, and our results show that they can represent a promising source of bioactive compounds.


Asunto(s)
Antiinfecciosos/farmacología , Bacterias/efectos de los fármacos , Biodiversidad , Hongos/efectos de los fármacos , Hongos/fisiología , Plantas Medicinales/microbiología , Solanum/microbiología , Antiinfecciosos/aislamiento & purificación , Brasil , Mezclas Complejas/farmacología , ADN Espaciador Ribosómico/genética , Endófitos , Hongos/química , Hongos/clasificación , Hongos/genética , Hongos/aislamiento & purificación , Genes de ARNr/genética , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Filogenia
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