RESUMEN

A viable human cytomegalovirus (HCMV) mutant was generated harbouring a glycoprotein B (gB) in which the carboxyl-terminal amino acids DRLRHR (aa 885-900) were changed to AALREE. Characterization of the phenotype of the recombinant virus revealed significant reduction of infectious progeny release and only moderate reduction of viral DNA replication indicating its diminished specific infectivity. This observation was in line with immunogold labeling of extracellular virions demonstrating that the amount of gB protein was markedly reduced in the envelope of the mutant virus. Our results suggest that the conserved carboxyl-terminus of the gB molecule is critical for HCMV maturation.

Asunto(s)

Citomegalovirus/genética , Proteínas del Envoltorio Viral/genética , Adaptación Fisiológica , Células Cultivadas , Citomegalovirus/patogenicidad , Fibroblastos , Humanos , Mutagénesis Sitio-Dirigida , Mutación , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/fisiología , Replicación Viral

Asunto(s)

Cloranfenicol O-Acetiltransferasa/genética , Genes Reporteros , Proteínas de Saccharomyces cerevisiae , Astrocitoma , Cromatografía en Capa Delgada , Técnicas de Cocultivo , Citomegalovirus/genética , Proteínas de Unión al ADN , Proteínas Fúngicas/genética , Proteína Vmw65 de Virus del Herpes Simple/genética , Humanos , Polietilenglicoles/farmacología , Regiones Promotoras Genéticas , Proteínas Recombinantes de Fusión/genética , Virus 40 de los Simios/genética , Factores de Transcripción/genética , Células Tumorales Cultivadas