RESUMEN
The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae. All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.
Asunto(s)
Mycobacterium tuberculosis , Neisseria gonorrhoeae , Azitromicina , Marcaje Isotópico , Lisina , Pruebas de Sensibilidad Microbiana , Antibacterianos/farmacología , Ciprofloxacina/farmacología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Medios de Cultivo CondicionadosRESUMEN
We use a mixed-spin model, with aperiodic ferromagnetic exchange interactions and crystalline fields, to investigate the effects of deterministic geometric fluctuations on first-order transitions and tricritical phenomena. The interactions and the crystal-field parameters are distributed according to some two-letter substitution rules. From a Migdal-Kadanoff real-space renormalization-group calculation, which turns out to be exact on a suitable hierarchical lattice, we show that the effects of aperiodicity are qualitatively similar for tricritical and simple critical behavior. In particular, the fixed point associated with tricritical behavior becomes fully unstable beyond a certain threshold dimension (which depends on the aperiodicity), and is replaced by a two-cycle that controls a weakened and temperature-depressed tricritical singularity.
RESUMEN
Five patients in early childhood had moderate to marked anemia and clinically demonstrable thick long bones of the extremities with radiologic features of diaphyseal dysplasia. Although the anemia was persistent and not responsive to hematinics, prednisolone was administered to two of these patients and caused remarkable improvement of the anemia in both. Roentgenologic examination carried out after 1 year of corticosteroid therapy in one patient and after 11 years in another showed considerable improvement of the bony changes. The clinical and radiologic examinations of these patients and their follow-up study suggest that they may constitute an unusual group of patients with diaphyseal dysplasia associated with anemia, hitherto not well defined.
Asunto(s)
Anemia/complicaciones , Síndrome de Camurati-Engelmann/complicaciones , Osteocondrodisplasias/complicaciones , Anemia/diagnóstico por imagen , Síndrome de Camurati-Engelmann/diagnóstico por imagen , Preescolar , Femenino , Estudios de Seguimiento , Humanos , Lactante , Masculino , RadiografíaRESUMEN
Glyoxalase polymorphism has been studied in 7296 persons from populations in South Asia, Southeast Asia, Oceania, Iran and Colombia. The GLO frequencies are very low in most of Oceania, including Australia, somewhat higher in Southeast Asia, and intermediate though variable in India. In Iran the GLO frequency is similar to that in Europe. The value for the single amerindian group in Colombia is nearly 30%.
Asunto(s)
Eritrocitos/enzimología , Lactoilglutatión Liasa/genética , Liasas/genética , Polimorfismo Genético , Asia Sudoriental , Asia Occidental , Australia , Colombia , Asia Oriental , Humanos , Lactoilglutatión Liasa/sangre , Islas del Pacífico , FenotipoRESUMEN
Escherichia coli O 27 H 7 was found in 16 stool samples submitted during a Caribbean cruise (Cruise Z) by 29 patients reporting with diarrhoea. A retrospective search revealed E. coli O 27 H 7 in 11 of 20 and 2 of 14 stool cultures from patients on two previous cruises (Y and X respectively) and in a culture from fresh cream (Cruise Y). The repeated occurrence of E. coli O 27 H 7 in the absence of any other apparent cause suggested that this serotype may have been responsible for the diarrhoea. The results of pathogenicity tests suggested that this strain elaborated heat-stable (ST) enterotoxin. The possibility that food may have been the vector is discussed.