RESUMEN
Within the complex mechanism of hepatic encephalopathy resulting from alcohol poisoning, a significant pathogenic role seems to relate to lipid peroxides (free radicals generated by chronic alcohol poisoning itself). Oxidative aggression is emphasized by the diminishing antioxidative capacity of the body resulting from serious liver injury brought about by chronic ethyl poisoning. The poisoning influences the whole functional capacity of the liver, hepatic protein synthesis included, which also means ceruloplasmin and siderophilin synthesis; the two latter elements make up AOS Cp-Tr which neutralizes the harmful activity of Cu and Fe excess and stimulates release of free radicals of oxygen. The purpose of our research was to study the redox balance alteration in blood in a group of patients who were in a critical condition and had been admitted to the somatic patients ward of the "Prof. Dr. Alexandru Obregia" Neurology and Psychiatry Hospital and who were suffering from hepatic encephalopathy resulting from ethyl poisoning.
Asunto(s)
Encefalopatía Hepática/metabolismo , Estrés Oxidativo , Intoxicación Alcohólica , Alcoholismo/metabolismo , Ceruloplasmina/análisis , Glutatión/sangre , Encefalopatía Hepática/inducido químicamente , Humanos , Hierro/sangre , Peróxidos Lipídicos/sangre , Persona de Mediana Edad , Especies Reactivas de Oxígeno/metabolismo , Superóxido Dismutasa/sangre , Transferrina/análisisRESUMEN
The clinical and experimental investigations of alcoholic liver disease, have proved that hepatocytes injury is associated with immunologic abnormalities, which can be one of the sources of these injuries. The acetaldehyde, the main ethanol metabolite modify liver cytosolic proteins and form new antigens. Thus acetaldehyde induces the synthesis of auto-antibodies. Some hypotheses maintain the autoimmune pathogenesis of these diseases. Research was carried out on some patient groups admitted in the alcohol addiction department of the Neurology and Psychiatry hospital "Professor Gh. Marinescu". We have investigated the alcohol depending individuals with various degrees of hepatic injuries. Four groups have been studied: the control group, the alcohol depending patients without liver disease, the patients with alcoholic hepatitis, the group with alcoholic cirrhosis. In order to investigate the immunologic response the research has been carried on, along the following lines: serum immunoglobulins level, serum C3 level, serum protein C reactive level by using IDR, serum circulating immune complexes by Hakova method, auto-antibodies (antinuclear AAN, antimitochondria AM and antismoothmuscle ASM) by using indirect immunofluorescence, T lymphocytes by E rosetting and lymphocytes by direct immuno-fluorescence. Clinical and experimental study of the alcoholic liver has shown that destruction of hepatocytes is accompanied by several immunological events.
Asunto(s)
Alcoholismo/inmunología , Hepatitis Alcohólica/inmunología , Inmunidad/fisiología , Cirrosis Hepática Alcohólica/inmunología , Caracteres Sexuales , Acetaldehído/inmunología , Anciano , Formación de Anticuerpos , Estudios de Casos y Controles , Femenino , Humanos , Inmunidad Celular , Masculino , Persona de Mediana EdadRESUMEN
The raport focuses on investigation of a sample of old people, mainly in their eighties, as against a control group of healthy subjects, aged between 25 and 40. In order to investigate the immunologic response the research has been carried on along the following lines. to show self Ab antinuclear (AAN), antimitochondrial (AM) and antismoth muscle (ASM), by using indirect immunofluorescence; to study alteration of serum immunoglobulins by using IDR; to study alteration of serum concentration of C3 fraction in the complement by using IDR; to determine immune complexes by using Haskova method; to investigate IR through R rosetting method. Immunologic reactivity (IR) disturbance was found, with respect to alteration of immunoglobulin ratio IgA, IgG, IgM vere slightly diminished. Self antibodies were present to a percentage of 17% as predominant of ASM, AM and AAN in decreasing order. Increase in C3 associated with increase in immune complexes could account for high incidence of cardio-vascular complications. Decline in IR is shown through the decrease in T lymphocytes where Ts is more markedly decreased as against decreased Ts. The results imbalance between stimulating activated cells and suppressive controlling cells. IR alteration could account for degenerative pathology in the subjects, namely, ATS, cardiovascular diseases, senility, pseudobulb syndrome, schizoaffective psychosis.
Asunto(s)
Envejecimiento/inmunología , Fosfatasa Ácida/sangre , Adulto , Anciano , Anciano de 80 o más Años , Autoanticuerpos/sangre , Proteínas Sanguíneas/metabolismo , Proteína C-Reactiva/metabolismo , Relación CD4-CD8 , Complemento C3/metabolismo , Femenino , Humanos , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Recuento de Linfocitos , Masculino , gammaglobulinas/metabolismoRESUMEN
In a study carried out in a group of 61 pregnant women in the 21st to the 41st week of gestation and comparatively in 9 nonpregnant controls, the following were determined: changes in the leukocyte and lymphocyte count, proportion of T cell rosettes, T1 rosettes (TM cells), T2 rosettes (TG cells), EAC rosettes (B cells), EACD rosettes (killer cells), circulating NBT-positive phagocytes, serum IgG, IgA, IgM and C3 concentrations, 50% hemolytic activity of the complement system. The results are interpreted in the light of present data regarding the mechanisms of ovo-embryo-fetal allograft.