RESUMEN
In vitro studies on the effect of alcoholic extracts of turmeric (TE), turmeric oil (TO) and turmeric oleoresin (TOR), on the incidence of micronuclei (Mn) in lymphocytes from normal healthy subjects showed that the test compounds did not cause any increase in the number of Mn as compared with those found in untreated controls. Further it was observed that all three compounds offered protection against benzo[a]pyrene induced increase in Mn in circulating lymphocytes. In subsequent studies, patients suffering from submucous fibrosis were given a total oral dose of TO (600 mg TO mixed with 3 g TE/day). TOR (600 mg + 3 g TE/day) and 3 g TE/day as a control for 3 months. It was observed that all three treatment modalities decreased the number of micronucleated cells both in exfoliated oral mucosal cells and in circulating lymphocytes. TOR was found to be more effective in reducing the number of Mn in oral mucosal cells (P < 0.001), but in circulating lymphocytes the decrease in Mn was comparable in all three groups.
Asunto(s)
Micronúcleos con Defecto Cromosómico/efectos de los fármacos , Neoplasias de la Boca/genética , Extractos Vegetales/farmacología , Lesiones Precancerosas/genética , Adolescente , Adulto , Curcuma , Daño del ADN , Humanos , MasculinoRESUMEN
One hundred patients suffering from oral submucous fibrosis (OSF), oral leukoplakia (OL) and oral lichen planus (OLP) were studied for the cytogenetic damage in oral mucosal cells and in circulating lymphocytes along with their habit patterns. It was observed that OSF was largely associated with betel nut containing masticants while OL was associated with chewing or smoking habit. It was further observed that their exfoliated oral mucosal cells had significantly higher numbers of micronucleated (Mn) cells as compared to these of healthy normal subjects without any chewing or smoking habit. Similar cytogenetic damage in the form of increased sister chromatid exchanges (SCE) was observed in circulating lymphocytes indicating that the carcinogenic agents produce damage not only in target tissue but also in other host cells such as circulating lymphocytes.
Asunto(s)
Liquen Plano/patología , Neoplasias de la Boca/patología , Lesiones Precancerosas/patología , Adolescente , Adulto , Areca , Daño del ADN , Femenino , Fibrosis/epidemiología , Fibrosis/patología , Humanos , India/epidemiología , Leucoplasia Bucal/epidemiología , Leucoplasia Bucal/patología , Liquen Plano/epidemiología , Linfocitos/patología , Masculino , Pruebas de Micronúcleos , Persona de Mediana Edad , Enfermedades de la Boca/epidemiología , Enfermedades de la Boca/patología , Mucosa Bucal/patología , Neoplasias de la Boca/epidemiología , Plantas Medicinales , Lesiones Precancerosas/epidemiologíaRESUMEN
Human lymphocytes were used as an assay system to test chemopreventive activity of natural products. Purnark, a mixture of extracts of turmeric, betel leaf and catechu, was tested for its chemoprotective activity against BP induced DNA damage. Sister chromatid exchange and micronuclei were used as markers to assess the protective activity of Purnark. Purnark gave 50-60% protection against BP induced SCEs and micronuclei. Purnark at 100 micrograms dose did not show any genotoxicity.