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STATEMENT OF PROBLEMS: An ideal antimicrobial agent should have minimal cytotoxic effect to host cells. PURPOSE: The aim of this study was to determine the cytotoxic effect of three commercial mouthwashes (Chlorhexidine, Persica and Irsha) on the cultured fibroblasts. MATERIAL AND METHODS: For determining the cytotoxic effect of Irsha, Chlorhexidine and Persica, uninfected cells were grown in the absence and presence of various concentration (2,4,8,16,32,64,128) of these mouth washes for 1, 2, 3 and 4 days. RESULTS: In this study, three mouth washes show cytotoxic effect on the cultured cells, at commercially available concentration and even diluted and Irsha was found to be the most toxic one. Cytotoxicity of three mouthwashes was reduced with decreasing concentration.
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BACKGROUND: Oral Lichen planus (OLP) is a chronic lesion of the oral mucosa with unknown origin. Basement membrane changes are common in OLP and may be mediated by proteases such as matrix metalloproteinase (MMPs) and mast cell chymase. The aim of our study was to evaluate the level of serum MMP-3 in OLP com-pared to normal individuals and assess its clinical significance. METHODS: Thirty four serum samples from patients diagnosed with OLP (12 males, 22 females, age: 42.2±10.8 years) and 34 serum samples from healthy control subjects (11 males, 23 females, age: 42.5±13.3 years) were collected and MMP-3 concentration was measured by ELISA. RESULTS: The serum MMP-3 level in OLP patients was higher (21.64±24.31 ng/ml) compared with healthy con-trols (16.52±23.63 ng/ml), but showed no statistically significant difference. A statistically significant difference was demonstrated between the two types of OLP, being more pronounced in the erosive/atrophic form 6). CONCLUSION: The different clinical appearances of OLP are associated with significant differences in MMP-3 serum level.
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BACKGROUND: Diabetes mellitus (DM) is one of the most common disorders of endocrine glands which has a worldwide distribution and is a risk factor for oral pathology so; the purpose of the present study was to investigate the association between median rhomboid glossitis (MRG) and DM. METHODS: We examined 202 Iranian patients with DM aged 10-86 years and 261 healthy subjects aged 10-28 years and the diagnosis of MRG was made based on clinical features. RESULTS: The examination indicated that 13 (6.43%) diabetic patients and 4 (1.53%) of control group had MRG.There was a significant difference in the prevalence of MRG, between patients and control group. MRG showed no association with other variables (age, sex, duration of DM, drugs, FBS, A1C). CONCLUSION: In the present study the prevalence of MRG in diabetics was much higher than that of controls.
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BACKGROUND: It was shown that two parasites of Entamoeba gingivalis (E. gingivalis) and Trichomonas tenax (T. tenax) may be responsible for oral parasitic infection. This study was designed to evaluate the prevalence of these parasites in oral cavity of patients with periodontal disease and in healthy population in Shiraz, Southern Iran. MATERIALS AND METHODS: A total of 50 patients with periodontal disease (case group) and 50 subjects with healthy gingiva (control group) entered in the present study. A questionnaire recorded general health, smoking habits, and any history of antibiotic consumption during the last six months for each patient. In the case group, saliva was collected by sterile swab and the gingival crevicular fluid by the paper point. The plaque and calculi were collected by sterile curette and scaler. In the control group, saliva and gingival crevicular fluid were collected and sent to laboratory for further studies. RESULTS: In the case group, nine patients were infected, six with E. gingivalis and three with T. tenax. Seven patients had mobility of the teeth, one patient was smoker and five had previous history of antibiotic consumption. In the control group, only one subject was infected with E. gingivalis without any history of smoking and antibiotic consumption. CONCLUSION: Parasitic infections are relatively common in patients with periodontal disease. It seems that follow-up of instructions are essential in control of parasitic infection in Southern Iran.