RESUMEN

To study the effect of simian vacuolating virus 40 (SV40) on development and differentiation of dendritic cells (DC) from rhesus macaque, the peripheral blood-derived dendritic cells from rhesus monkey were pulsed with inactivated SV40 and infective SV40, respectively at the 5th day post DC cultivation. Expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface at the 7th, 9th day post DC cultivation were analyzed by flow cytometry (FCM). The results showed that expressions of CD1a, HLA-DR, CD86 and CD83 on the cell surface in the inactivated SV40-pulsed experimental group were higher than those in the infective SV40-pulsed experimental group (P < 0.05). These cell surface molecules represented characteristic development and differentiation phase of DC. Down-regulation of expressions of these cell surface molecules indicated that infective SV40 might hamper differentiation and maturation of dendritic cells from rhesus monkey.

Asunto(s)

Diferenciación Celular , Células Dendríticas/citología , Células Dendríticas/virología , Virus 40 de los Simios/fisiología , Animales , Antígenos CD/metabolismo , Antígenos CD1/metabolismo , Antígeno B7-2/metabolismo , Células Cultivadas , Células Dendríticas/inmunología , Citometría de Flujo , Antígenos HLA-DR/metabolismo , Inmunoglobulinas/metabolismo , Macaca mulatta , Glicoproteínas de Membrana/metabolismo , Infecciones por Polyomavirus/fisiopatología , Antígeno CD83