RESUMEN
Tissue functions and mechanical coupling of cells must be integrated throughout development. A striking example of this coupling is the interactions of body wall muscle and hypodermal cells in Caenorhabditis elegans. These tissues are intimately associated in development and their interactions generate structures that provide a continuous mechanical link to transmit muscle forces across the hypodermis to the cuticle. Previously, we established that mup-4 is essential in embryonic epithelial (hypodermal) morphogenesis and maintenance of muscle position. Here, we report that mup-4 encodes a novel transmembrane protein that is required for attachments between the apical epithelial surface and the cuticular matrix. Its extracellular domain includes epidermal growth factor-like repeats, a von Willebrand factor A domain, and two sea urchin enterokinase modules. Its intracellular domain is homologous to filaggrin, an intermediate filament (IF)-associated protein that regulates IF compaction and that has not previously been reported as part of a junctional complex. MUP-4 colocalizes with epithelial hemidesmosomes overlying body wall muscles, beginning at the time of embryonic cuticle maturation, as well as with other sites of mechanical coupling. These findings support that MUP-4 is a junctional protein that functions in IF tethering, cell-matrix adherence, and mechanical coupling of tissues.
Asunto(s)
Proteínas de Caenorhabditis elegans , Caenorhabditis elegans/metabolismo , Moléculas de Adhesión Celular/metabolismo , Células Epiteliales/metabolismo , Animales , Animales Modificados Genéticamente , Caenorhabditis elegans/embriología , Caenorhabditis elegans/crecimiento & desarrollo , Adhesión Celular/fisiología , Moléculas de Adhesión Celular/genética , Clonación Molecular , Embrión no Mamífero/metabolismo , Células Epiteliales/citología , Expresión Génica/efectos de los fármacos , Proteínas Fluorescentes Verdes , Proteínas del Helminto/genética , Proteínas del Helminto/metabolismo , Hemidesmosomas/metabolismo , Larva/metabolismo , Larva/ultraestructura , Proteínas Luminiscentes/genética , Proteínas de la Membrana/metabolismo , Datos de Secuencia Molecular , Músculos/metabolismo , Músculos/ultraestructura , Mutación , Especificidad de Órganos , Mapeo Físico de Cromosoma , ARN Bicatenario/farmacología , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
Comparative maps have been a valuable resource for extrapolating biological information among organisms. The relationship between mouse and human maps provides a framework for integrating information from each species and thereby increasing the utility of all available data such as gene location, structure and function. This review describes the various public resources, both databases and web sites, containing genome-wide mouse-human comparative map information available through the World-Wide Web. We will focus on the use and applicability of these resources in their current form and consider future potential directions.
Asunto(s)
Bases de Datos Factuales , Genoma Humano , Genoma , Internet , Ratones/genética , Animales , Biología Computacional/métodos , Biología Computacional/tendencias , ADN/química , ADN/genética , Ligamiento Genético , Humanos , Homología de Secuencia de Ácido NucleicoRESUMEN
mup-4 is a member of a set of genes essential for correct embryonic body wall muscle cell positions in Caenorhabditis elegans. The mup-4 phenotype is variably expressed and three discrete arrest phenotypes arise during the phase of embryonic development when the worm elongates from a ball of cells to its worm shape (organismal morphogenesis). Mutants representing two of the phenotypic classes arrest without successful completion of elongation. Mutants of the third phenotypic class arrest after completion of elongation. Mutants that arrest after elongation display profound dorsal and ventral body wall muscle cell position abnormalities and a characteristic kinked body shape (the Mup phenotype) due to the muscle cell position abnormalities. Significantly, genetic mosaic analysis of mup-4 mutants demonstrates that mup-4 gene function is essential in the AB lineage, which generates most of the hypodermis (epidermis), a tissue with which muscle interacts. Consistent with the genetic mosaic data, phenotypic characterizations reveal that mutants have defects in hypodermal integrity and morphology. Our analyses support the conclusion that mup-4 is essential for hypodermal function and that this function is necessary for organismal morphogenesis and for the maintenance of body wall muscle position.