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Fungal Biol ; 120(4): 500-512, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-27020152

RESUMEN

Quantitative transcriptome analysis led to the identification of 331 transcripts regulated by white light. Evaluation of the response to white light in mutants affected in the previously characterized blue-light receptor Blr1, demonstrated the existence of both Blr1-dependent and independent responses. Functional categorization of the light responsive genes indicated the effect of light on regulation of various transcription factors, regulators of chromatin structure, signaling pathways, genes related to different kinds of stress, metabolism, redox adjustment, and cell cycle among others. In order to establish the participation of other photoreceptors, gene expression was validated in response to different wavelengths. Gene regulation by blue and red light suggests the involvement of several photoreceptors in integrating light signals of different wavelengths in Trichoderma atroviride. Functional analysis of potential blue light photoreceptors suggests that several perception systems for different wavelengths are involved in the response to light. Deletion of cry1, one of the potential photoreceptors, resulted in severe reduction in the photoreactivation capacity of the fungus, as well as a change in gene expression under blue and red light.


Asunto(s)
Criptocromos/metabolismo , Desoxirribodipirimidina Fotoliasa/metabolismo , Regulación Fúngica de la Expresión Génica/efectos de la radiación , Luz , Trichoderma/genética , Trichoderma/efectos de la radiación , Proteínas Fúngicas/genética , Perfilación de la Expresión Génica , Factores de Transcripción/genética
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