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1.
BMC Zool ; 9(1): 26, 2024 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-39390615

RESUMEN

BACKGROUND: The tropical gar (Atractosteus tropicus), a member of the Lepisosteidae family, is native to regions extending from southeastern Mexico to southern Costa Rica. This species serves as a unique bridge between tetrapods and teleosts due to its phylogenetic position, slow evolutionary rate, dense genetic map, gene similarities with humans, and ease of laboratory cultivation. As a taxonomic sister group to teleosts like the zebrafish (Danio rerio), known for its high regenerative capacity, it remains unclear whether the tropical gar shares a similar ability for regeneration. RESULTS: This study aims to elucidate the caudal fin regeneration process in tropical gar through skeletal and histological staining methods. Juvenile specimens were observed over a two-month period, during which they were fed brine shrimp, and anesthetized with 1% eugenol for caudal fin amputation. Samples were collected at various days post-amputation (dpa). Alcian blue and alizarin red staining were used to highlight skeletal regeneration, particularly the formation of new cartilage, while histological staining with hematoxylin and eosin was performed to observe tissue regeneration at the amputation site. CONCLUSIONS: The findings reveal a remarkable ability for caudal fin regeneration in juvenile tropical gar. Given the Garfish evolutionary relationship with teleosts, this opens new avenues for research into tissue regeneration across different groups of Actinopterygii.

2.
Animals (Basel) ; 14(18)2024 Sep 13.
Artículo en Inglés | MEDLINE | ID: mdl-39335253

RESUMEN

Probiotics in aquaculture hold promise for enhancing fish health and growth. Due to their increased specificity and affinity for their host, indigenous probiotics may offer isolated and potentially amplified benefits. This study investigated the effects of Lactococcus lactis PH3-05, previously isolated from adults of tropical gar (Atractosteus tropicus), on the growth, survival, digestive enzyme activity, intestinal morphology, expression of barrier and immune genes, and intestinal microbiota composition in the larvae of tropical gar. Larvae were fed with live L. lactis PH3-05 concentrations of 104, 106, and 108 CFU/g for 15 days alongside a control diet without probiotics. Higher concentrations of L. lactis PH3-05 (106 and 108 CFU/g) positively influenced larval growth, increasing hepatocyte area and enterocyte height. The 106 CFU/g dose significantly enhanced survival (46%) and digestive enzyme activity. Notably, the 108 CFU/g dose stimulated increased expression of muc-2 and il-10 genes, suggesting enhanced mucosal barrier function and anti-inflammatory response. Although L. lactis PH3-05 did not significantly change the diversity, structure, or Phylum level composition of intestinal microbiota, which was constituted by Proteobacteria, Bacteroidota, Chloroflexi, and Firmicutes, an increase in Lactobacillus abundance was observed in fish fed with 106 CFU/g, suggesting enhanced probiotic colonization. These results demonstrate that administering L. lactis PH3-05 at 106 CFU/g promotes growth, survival, and digestive health in A. tropicus larvae, establishing it as a promising indigenous probiotic candidate for aquaculture applications.

3.
Int J Biol Macromol ; 268(Pt 1): 131861, 2024 May.
Artículo en Inglés | MEDLINE | ID: mdl-38670207

RESUMEN

This study characterized four corrole derivatives, namely Cbz-Cor, MetCbz-Cor, PTz-Cor, and PTzEt-Cor, examining their photophysical, electrochemical, photobiological, and biomolecule-binding properties. Experimental photophysical data of absorption and emission elements correlated with a theoretical analysis obtained through time-dependent density functional theory (TD-DFT). As for the photophysical properties, we observed lower fluorescence quantum yields and discernible differences between the excited and ground states, as indicated by Stokes shift values. Natural Transition Orbit (NTO) plots presented high occupied molecular orbital - low unoccupied molecular orbital (HOMO-LUMO) densities around the tetrapyrrolic macrocycle in all examples. Our findings demonstrate that corroles maintain stability in solution and offer photostability (<20 %), predominantly in DMSO(5 %)/Tris-HCl (pH 7.4) buffer solution. Furthermore, the singlet oxygen (1O2) quantum yield and log POW values underscore their potential application in photoinactivation approaches, as these corroles serve as effective ROS generators with more lipophilic features. We also evaluated their biomolecular binding capacity towards salmon sperm DNA and human serum albumin using spectroscopic techniques and molecular docking analysis for sustenance. Concerning biomolecule interaction profiles, the corrole derivatives showed a propensity for interacting in the minor grooves of the double helix DNA due to secondary forces, which were more pronounced in site III of the human serum protein.


Asunto(s)
Carbazoles , ADN , Fenotiazinas , Albúmina Sérica Humana , ADN/química , Fenotiazinas/química , Humanos , Carbazoles/química , Albúmina Sérica Humana/química , Albúmina Sérica Humana/metabolismo , Porfirinas/química , Animales , Unión Proteica , Salmón , Simulación del Acoplamiento Molecular , Oxígeno Singlete/química , Oxígeno Singlete/metabolismo
4.
Salud Colect ; 20: e4648, 2024 Feb 12.
Artículo en Español | MEDLINE | ID: mdl-38376859

RESUMEN

Women drug-users face significant challenges in accessing and adhering to outpatient treatment. However, this issue has been underexplored. The study aims to understand the experiences of a group of women undergoing drug use treatment. Between March and December 2021, a qualitative phenomenological interpretative study was carried out through semi-structured interviews with 16 women attending a drug-dependence care center in Catalonia, Spain. Data were processed through content analysis. The results indicate that, despite perceiving a positive impact of the treatment, women experience vulnerabilities related to stigmatization, lack of social support, and insufficient coverage of specific needs, impacting therapeutic follow-up. The findings emphasize the need to enhance resources for specialized care and promote a social support network where women drug-users actively participate.


Las mujeres consumidoras de drogas se enfrentan a grandes desafíos en el acceso y la adherencia al tratamiento ambulatorio. Sin embargo, esta cuestión ha sido poco estudiada. El objetivo del estudio es comprender las experiencias de un grupo de mujeres en tratamiento por consumo de drogas. Entre marzo y diciembre de 2021, se realizó un estudio cualitativo fenomenológico interpretativo mediante entrevistas semiestructuradas a 16 mujeres usuarias de un centro de atención a las drogodependencias de Cataluña (España). Los datos se procesaron mediante el análisis de contenido. Los resultados indican que las mujeres, pese a percibir un impacto positivo del tratamiento, viven realidades de vulnerabilidad relacionadas con la estigmatización, la falta de apoyo social y una baja cobertura de necesidades específicas con implicaciones en el seguimiento terapéutico. Los hallazgos enfatizan la necesidad de mejorar los recursos para una atención especializada y promover una red de apoyo social donde participen activamente las mujeres consumidoras de drogas.


Asunto(s)
Consumidores de Drogas , Pacientes Ambulatorios , Femenino , Humanos , Salud Mental , Investigación Cualitativa , Apoyo Social
5.
Curr Probl Cardiol ; 49(3): 102392, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38232925

RESUMEN

OBJECTIVES: A systematic review of the literature was conducted to analyze the current evidence on low-flow, low-gradient severe aortic stenosis. This analysis aimed to differentiate between subgroups of patients with reduced and preserved left ventricular ejection fraction (LVEF). METHODS: After conducting a systematic literature review, 35 observational studies were included. Out of these, 28 were prospective and 7 retrospective. The studies that included a mortality risk stratification of low-flow, low-gradient aortic stenosis (LF- LG AS) with both preserved and reduced LVEF were reviewed. RESULTS: The importance of considering multiple clinical and echocardiographic variables in diagnostic evaluation and therapeutic decision-making was highlighted. CONCLUSIONS: LF- LG AS, in any of its subgroups, is a common and challenging valve lesion. A careful assessment of severity and, in specific scenarios, a thorough reclassification is important. More high-quality studies are required to more precisely define the classification and prognosis of this entity.


Asunto(s)
Estenosis de la Válvula Aórtica , Volumen Sistólico , Función Ventricular Izquierda , Humanos , Válvula Aórtica/fisiopatología , Válvula Aórtica/diagnóstico por imagen , Estenosis de la Válvula Aórtica/fisiopatología , Estenosis de la Válvula Aórtica/diagnóstico , Estenosis de la Válvula Aórtica/complicaciones , Ecocardiografía/métodos , Pronóstico , Índice de Severidad de la Enfermedad , Volumen Sistólico/fisiología , Función Ventricular Izquierda/fisiología
6.
Toxicon ; 239: 107616, 2024 Feb 23.
Artículo en Inglés | MEDLINE | ID: mdl-38218384

RESUMEN

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a key vector of the phloem-limited bacteria Candidatus Liberibacter asiaticus (CLas) associated with huanglongbing (HLB), the most serious and currently incurable disease of citrus worldwide. Here we report the first investigation into the potential use of a spider venom-derived recombinant neurotoxin, ω/κ-HxTx-Hv1h (hereafter HxTx-Hv1h) when delivered alone or when fused to snowdrop lectin (Galanthus nivalis agglutinin; GNA) to control D. citri. Proteins, including GNA alone, were purified from fermented transformed yeast Pichia pastoris cultures. Recombinant HxTx-Hv1h, HxTx-Hv1h/GNA and GNA were all orally toxic to D. citri, with Day 5 median lethal concentrations (LC50) derived from dose-response artificial diet assays of 27, 20 and 52 µM, respectively. Western analysis of whole insect protein extracts confirmed that psyllid mortality was attributable to protein ingestion and that the fusion protein was stable to cleavage by D. citri proteases. When applied topically (either via droplet or spray) HxTx-Hv1h/GNA was the most effective of the proteins causing >70 % mortality 5 days post treatment, some 2 to 3-fold higher levels of mortality as compared to the toxin alone. By contrast, no significant mortality or phenotypic effects were observed for bumble bees (Bombus terrestris L.) fed on the recombinant proteins in acute toxicity assays. This suggests that HxTx-Hv1h/GNA has potential as a novel bioinsecticide for the management of D. citri offering both enhanced target specificity as compared to chemical pesticides and compatibility with integrated pest management (IPM) strategies.


Asunto(s)
Citrus , Hemípteros , Liberibacter , Animales , Hemípteros/fisiología , Neurotoxinas , Citrus/microbiología , Enfermedades de las Plantas/microbiología
7.
Motrivivência (Florianópolis) ; 36(67): 1-24, 2024.
Artículo en Portugués | LILACS-Express | LILACS | ID: biblio-1532980

RESUMEN

Objetivou-se levantar a produção científica na literatura sobre violência nas aulas de EF escolar nos últimos 5 anos. Desenvolveu-se uma revisão sistemática sobre a temática de violência nas áreas da Educação e EF através do método PRISMA. As bases consultadas foram Scielo, Lilacs, Scopus e Google Acadêmico, durante setembro/2022- outubro/2022. Foram encontrados 1028 estudos e, após aplicados os filtros de seleção, chegou-se a 14 trabalhos. Percebeu- se uma ascendência de pesquisas relacionadas à violência escolar e EF de cunho qualitativo e predominantes nas regiões Sudeste e Sul do Brasil. Os dados mostram que os cursos de formação de professores de EF ainda carecem de informações sobre o fenômeno da violência escolar, questão que afeta a vivência pedagógica dos docentes, uma vez que a postura do professor pode gerar e/ou intensificar atos de violência ao corpo docente e discente, tendo a escola, muitas vezes, um papel omisso frente à problemática.


The objective was to survey the scientific production in the literature on violence in PE classes at school in the last 5 years. A systematic review was developed on the theme of violence in the areas of Education and PE using the PRISMA method. The databases consulted were Scielo, Lilacs, Scopus and Google Scholar, during September/2022-October/2022. 1028 studies were found and, after applying the selection filters, 14 works were reached. An ascendance of research related to school violence and PE of a qualitative nature and predominant in the Southeast and South regions of Brazil was noticed. The data show that the training courses for PE teachers still lack information about the phenomenon of school violence, an issue that affects the pedagogical experience of teachers, since their attitude can generate and/or intensify acts of violence against teachers and students, with the school often having a silent role in the face of the problem.


El objetivo fue relevar la producción científica en la literatura sobre la violencia en las clases de EF en la escuela en los últimos 5 años. Se desarrolló una revisión sistemática sobre el tema de la violencia en las áreas de Educación y EF utilizando el método PRISMA. Las bases de datos consultadas fueron Scielo, Lilacs, Scopus y Google Scholar, durante septiembre/2022-octubre/2022. Se encontraron 1028 estudios y, después de aplicar los filtros de selección, se llegó a 14 trabajos. Se notó un ascenso de investigaciones relacionadas con la violencia escolar y la EF de carácter cualitativo y predominantes en las regiones Sureste y Sur de Brasil. Los datos muestran que los cursos de formación de docentes de EF aún carecen de información sobre el fenómeno de la violencia escolar, tema que afecta la experiencia pedagógica de los docentes, ya que la actitud del profesor puede generar y/o intensificar actos de violencia contra docentes y alumnos, teniendo la escuela muchas veces un papel silencioso frente al problema.

8.
Salud colect ; 20: 4648-4648, 2024.
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1560483

RESUMEN

RESUMEN Las mujeres consumidoras de drogas se enfrentan a grandes desafíos en el acceso y la adherencia al tratamiento ambulatorio. Sin embargo, esta cuestión ha sido poco estudiada. El objetivo del estudio es comprender las experiencias de un grupo de mujeres en tratamiento por consumo de drogas. Entre marzo y diciembre de 2021, se realizó un estudio cualitativo fenomenológico interpretativo mediante entrevistas semiestructuradas a 16 mujeres usuarias de un centro de atención a las drogodependencias de Cataluña (España). Los datos se procesaron mediante el análisis de contenido. Los resultados indican que las mujeres, pese a percibir un impacto positivo del tratamiento, viven realidades de vulnerabilidad relacionadas con la estigmatización, la falta de apoyo social y una baja cobertura de necesidades específicas con implicaciones en el seguimiento terapéutico. Los hallazgos enfatizan la necesidad de mejorar los recursos para una atención especializada y promover una red de apoyo social donde participen activamente las mujeres consumidoras de drogas.


ABSTRACT Women drug-users face significant challenges in accessing and adhering to outpatient treatment. However, this issue has been underexplored. The study aims to understand the experiences of a group of women undergoing drug use treatment. Between March and December 2021, a qualitative phenomenological interpretative study was carried out through semi-structured interviews with 16 women attending a drug-dependence care center in Catalonia, Spain. Data were processed through content analysis. The results indicate that, despite perceiving a positive impact of the treatment, women experience vulnerabilities related to stigmatization, lack of social support, and insufficient coverage of specific needs, impacting therapeutic follow-up. The findings emphasize the need to enhance resources for specialized care and promote a social support network where women drug-users actively participate.

9.
Injury ; 54 Suppl 6: 110723, 2023 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-38143141

RESUMEN

A proper evaluation of the narrowings and length of the anterior acetabular column would offer better predictability and precision for implant insertion in the case of an acetabular fracture. OBJECTIVE: To determine the diameter and length of the safety corridor of the anterior column of the acetabulum in patients with available pelvic computed tomography (CT), analyze the obtained measurements against those of a standard 6.5-mm implant, and verify possible sex differences regarding these measurements. A secondary aim was to develop a method for measurement of the anterior column of the acetabulum based on CT images. MATERIALS AND METHODS: In 200 CT scans of hemipelvises we measured the diameter of two areas of narrowing and the length of the safety corridor of the anterior column. The images were submitted to multiplanar reformatting adjusted to a plane orthogonal to the bone corridor, drawn at the level of the superior pubic ramus. RESULTS: Measurement #1 had a mean value of 8.12 (2.27) mm in the overall sample and median values of 9.03 (7.76-10.48) mm in men and 6.77 (5.44-7.19) mm in women. Measurement #2 had a mean value of 7.29 (2.19) mm and median values of 8.23 (7.18-9.82) mm in men and 5.9 (4.65-7.19) mm in women. Measurement #3 had a mean value of 109.53 (13.66) mm in the overall sample and median values of 117.17 (112.9-122.9) mm in men and 100.91 (90.95-111.17) mm in women (p<0.001 all three measurements). Measurement #1 was smaller than 6.5 mm in 22.5% of the patients (of whom 90% were women). Measurement #2 was smaller than 6.5 mm in 35% of the patients (of whom 80% were women). CONCLUSIONS: This study proposed an anatomic evaluation of the anterior column of the acetabulum using conventional CT images The areas of narrowing in the anterior column had an average of 8.12 mm at the level of the pubic tubercle and 7.29 mm at the level of the acetabular fossa. The mean length of the safety corridor was 109.53 mm. In 35% of the cases, a 6.5 mm percutaneous screw would have violated the cortical bone of the safety corridor.


Asunto(s)
Fracturas Óseas , Fracturas de Cadera , Fracturas de la Columna Vertebral , Humanos , Masculino , Femenino , Fijación Interna de Fracturas/métodos , Proyectos Piloto , Fracturas de Cadera/cirugía , Acetábulo/diagnóstico por imagen , Acetábulo/cirugía , Acetábulo/lesiones , Tornillos Óseos , Fracturas Óseas/diagnóstico por imagen , Fracturas Óseas/cirugía
10.
EJIFCC ; 34(3): 228-244, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37868088

RESUMEN

Background: Analyzing longitudinal gene expression data is extremely challenging due to limited prior information, high dimensionality, and heterogeneity. Similar difficulties arise in research of multifactorial diseases such as Type 2 Diabetes. Clustering methods can be applied to automatically group similar observations. Common clinical values within the resulting groups suggest potential associations. However, applying traditional clustering methods to gene expression over time fails to capture variations in the response. Therefore, shape-based clustering could be applied to identify patient groups by gene expression variation in a large time metabolic compensatory intervention. Objectives: To search for clinical grouping patterns between subjects that showed similar structure in the variation of IL-1ß gene expression over time. Methods: A new approach for shape-based clustering by IL-1ß expression behavior was applied to a real longitudinal database of Type 2 Diabetes patients. In order to capture correctly variations in the response, we applied traditional clustering methods to slopes between measurements. Results: In this setting, the application of K-Medoids using the Manhattan distance yielded the best results for the corresponding database. Among the resulting groups, one of the clusters presented significant differences in many key clinical values regarding the metabolic syndrome in comparison to the rest of the data. Conclusions: The proposed method can be used to group patients according to variation patterns in gene expression (or other applications) and thus, provide clinical insights even when there is no previous knowledge on the subject clinical profile and few timepoints for each individual.

11.
J Appl Stat ; 50(6): 1255-1282, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37025282

RESUMEN

We introduce a new class of heteroscedastic partially linear model (PLM) with skew-normal distribution. Maximum likelihood estimation of the model parameters by the ECM algorithm (Expectation/Conditional Maximization) as well as influence diagnostics for the new model are investigated. In addition, a Likelihood Ratio test for assessing the homogeneity of the scale parameter is presented. Simulation studies for assessing the performance of the ECM algorithm and the Likelihood Ratio test statistics for homogeneity of variance are developed. Also, a study for misspecification of the structure function is considered. Finally, an application of the new heteroscedastic PLM to a real data set on ragweed pollen concentration is presented to show that it provides a better fit than the classic homocedastic PLM. We hope that the proposed model may attract applications in different areas of knowledge.

12.
Materials (Basel) ; 16(8)2023 Apr 21.
Artículo en Inglés | MEDLINE | ID: mdl-37110110

RESUMEN

Mg-Zn co-dopedGaN powders via the nitridation of a Ga-Mg-Zn metallic solution at 1000 °C for 2 h in ammonia flow were obtained. XRD patterns for the Mg-Zn co-dopedGaN powders showed a crystal size average of 46.88 nm. Scanning electron microscopy micrographs had an irregular shape, with a ribbon-like structure and a length of 8.63 µm. Energy-dispersive spectroscopy showed the incorporation of Zn (Lα 1.012 eV) and Mg (Kα 1.253 eV), while XPS measurements showed the elemental contributions of magnesium and zinc as co-dopant elements quantified in 49.31 eV and 1019.49 eV, respectively. The photoluminescence spectrum showed a fundamental emission located at 3.40 eV(364.70 nm), which was related to band-to-band transition, besides a second emission found in a range from 2.80 eV to 2.90 eV (442.85-427.58 nm), which was related to a characteristic of Mg-doped GaN and Zn-doped GaN powders. Furthermore, Raman scattering demonstrated a shoulder at 648.05 cm-1, which could indicate the incorporation of the Mg and Zn co-dopants atoms into the GaN structure. It is expected that one of the main applications of Mg-Zn co-doped GaN powders is in obtaining thin films for SARS-CoV-2 biosensors.

13.
Medisan ; 27(2)abr. 2023. tab
Artículo en Español | LILACS, CUMED | ID: biblio-1440579

RESUMEN

Introducción: El dolor es el síntoma más común luego de la primera activación de la aparatología ortodóncica. El tratamiento analgésico más utilizado es el paracetamol; sin embargo, su uso inhibe la actividad de la ciclooxigenasa y la síntesis de prostaglandinas, lo que puede afectar el mecanismo del movimiento dental y el remodelado óseo. Objetivo: Evaluar la efectividad del láser de baja potencia para el alivio del dolor en pacientes con tratamiento ortodóntico. Métodos: Se realizó un estudio cuasiexperimental de intervención terapéutica, desde enero hasta diciembre de 2020, en 40 pacientes atendidos en la consulta de ortodoncia del Hospital General Docente Dr. Juan Bruno Zayas Alfonso de Santiago de Cuba, en quienes se aplicaría fuerza ortodóncica por primera vez. Estos fueron distribuidos de forma aleatoria en 2 grupos de 20 integrantes cada uno: el de estudio, tratado con láser de baja potencia, y el de control, que recibió medicación convencional (paracetamol). Para la validación estadística de la información se emplearon el porcentaje y la prueba de la t de Student para muestras independientes, con 95 % de confiabilidad. Resultados: Luego de tres sesiones terapéuticas, 90,0 % del grupo de estudio refirió no presentar dolor o sentirlo de forma leve, mientras que del grupo de control solo 10,0 % de los pacientes fue ubicado en cualquiera de las dos categorías anteriores. Existió diferencia significativa entre ambos grupos (p=0,00). Conclusiones: Se demostró que el láser, como terapia alternativa, fue más efectivo que el paracetamol.


Introduction: Pain is the most common symptom after the first activation of orthodontic appliance. The most widely used analgesic treatment is paracetamol; however, its use inhibits cyclooxygenase activity and prostaglandin synthesis, which can affect the mechanism of tooth movement and bone remodeling. Objective: To evaluate the effectiveness of low power laser for pain relief in patients with orthodontic treatment. Methods: A quasi-experimental study of therapeutic intervention was carried out, from January to December 2020, in 40 patients treated at the orthodontic clinic of Dr. Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba, in whom orthodontic force was applied for the first time. These were randomly distributed into 2 groups of 20 members each: the study group, treated with low-power laser, and the control group, that received conventional medication (paracetamol). For statistical validation of data, the percentage and the Student's t test for independent samples were used, with 95 % confidence interval. Results: After three therapeutic sessions, 90.0% of the study group reported not presenting pain or feeling it slightly, while only 10.0% of patients in control group were put in any of the two previous categories. There was a significant difference between both groups (p=0.00). Conclusions: Laser, as an alternative therapy, was shown to be more effective than paracetamol.


Asunto(s)
Terapia por Luz de Baja Intensidad , Dolor Asociado a Procedimientos Médicos , Aparatos Ortodóncicos , Técnicas de Movimiento Dental , Analgesia
14.
Phys Chem Chem Phys ; 25(6): 5021-5028, 2023 Feb 08.
Artículo en Inglés | MEDLINE | ID: mdl-36722878

RESUMEN

Organic molecules have been intensively studied during the last few decades because of their photonics and biological applications. In this material class, the fluorene molecules present outstanding optical features, for example, high values of two-photon absorption (2PA) cross-sections, visible transparency, and high fluorescence quantum yield. Also, it is possible to improve the nonlinear optical response by modifying the fluorene molecular structure. In this context, herein, we have synthesized V and Y-shaped branching oligofluorenes containing two and three fluorene moieties in each branch. Such a molecular strategy may exponentially enhance the nonlinear optical response due to the coherent coupling among the molecular arms. Thus, we combined the use of femtosecond Z-scan spectroscopy and white light transient absorption spectroscopy (TAS) to understand the molecular structure and 2PA property relationship of branching oligofluorenes. The results show that there is a universal relationship between the 2PA cross-section and the effective π-electron number (Neff) given by σ2PA(GM) = (079 ± 0.03)Neff2, which is independent of the molecular shape (linear, V or Y-shaped). Therefore, the intramolecular charge transfer responsible for the cooperative effect among the branches does not occur. This statement is corroborated by the results of the femtosecond TAS technique.

15.
J Inorg Biochem ; 242: 112149, 2023 05.
Artículo en Inglés | MEDLINE | ID: mdl-36780772

RESUMEN

We report the synthesis and characterization of new tri-cationic corrole derivatives, containing Pt(II) or Pd(II) complexes attached at the peripheral position of thienyl moieties. Corrole derivatives were characterized through microanalysis, electrochemical, spectrometry and spectroscopy analysis. Singlet and triplet excited-states are investigated by photophysical/theoretical calculation methods and photobiological parameters were also evaluated spectroscopic techniques (UV-Vis and EPR). Also, the binding capacity of each corrole derivative with nucleic acids (DNA) and human serum albumin (HSA) was determined by UV-Vis, steady-state, and time-resolved fluorescence spectroscopy, combined with molecular docking analysis. Moreover, the new corroles containing peripheral complexes improve their interactions with biomacromolecules, generate reactive oxygen species under light source irradiation studied and has potential for application in photodynamic therapeutic processes.


Asunto(s)
Porfirinas , Humanos , Simulación del Acoplamiento Molecular , Porfirinas/química , Espectrometría de Fluorescencia
16.
Int J Biostat ; 19(2): 389-415, 2023 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-36279154

RESUMEN

Many health care professionals and institutions manage longitudinal databases, involving follow-ups for different patients over time. Longitudinal data frequently manifest additional complexities such as high variability, correlated measurements and missing data. Mixed effects models have been widely used to overcome these difficulties. This work proposes the use of linear mixed effects models as a tool that allows to search conceptually different types of anomalies in the data simultaneously.


Asunto(s)
Manejo de Datos , Humanos , Estudios Longitudinales , Modelos Lineales , Bases de Datos Factuales
18.
Saúde Soc ; 32(4): e220352pt, 2023. tab
Artículo en Portugués | LILACS | ID: biblio-1530420

RESUMEN

Resumo À luz das práticas de lazer, objetivamos analisar os sentidos do consumo de drogas por homens homossexuais em uma casa noturna da cidade do Rio de Janeiro, RJ. Realizamos o processo de observação participante em 36 episódios de festas eletrônicas em uma boate, entre janeiro de 2018 e setembro de 2019. Detectamos que os usos de substâncias estabelecem um sentimento de pertencimento social entre os sujeitos, funcionando como rede de apoio coesa e cerimonial. Concluímos que, para os interlocutores, as drogas assumem diferentes sentidos para um "bem-viver" coletivo, em que a droga simboliza a união e o apoio socioemocional que conecta os homossexuais consumidores em um espaço plural às diversidades, a princípio sem julgamentos morais. O consumo das drogas em cada festa fortaleceu a coesão grupal, além de refletir sentimentos de fraternidade, companheirismo e irmandade, edificando e retroalimentando uma espécie de "grupo terapêutico" atrelado a essa vivência do lazer, uma prática acionada para "suportar e sobreviver" às demandas sociais, por vezes preconceituosas e discriminatórias.


Abstract In light of leisure practices, we aim to analyze the meanings of drug consumption by homosexual men in a nightclub in the city of Rio de Janeiro, RJ. We carried out the participant observation process in 36 episodes of electronic parties in a nightclub, between January 2018 and September 2019. We detected that substance use establishes a sense of social belonging among the subjects, functioning as a cohesive and ceremonial support network. We conclude that, for those interlocutors, drugs take on different meanings for a collective "good living," in which the drug symbolizes the union and socio-emotional support that connects homosexual consumers in a plural space to diversities, at first without moral judgments. The consumption of drugs at each party strengthened group cohesion, in addition to reflecting feelings of fraternity, companionship, and brotherhood, building and feeding back a kind of "therapeutic group" linked to this experience of leisure, a practice activated to "endure and survive" the social demands, sometimes prejudiced and discriminatory.


Asunto(s)
Drogas Ilícitas , Homosexualidad Masculina , Investigación Cualitativa , Políticas Públicas de no Discriminación
19.
Movimento (Porto Alegre) ; 29: e29045, 2023. tab
Artículo en Portugués | LILACS-Express | LILACS | ID: biblio-1529032

RESUMEN

Resumo Hoje em dia, o cenário esportivo, sobretudo retratado pelas mídias, vem apresentando novas perspectivas em relação às práticas atlético-esportivas LGBTI+. O objetivo desta pesquisa foi analisar a cobertura televisiva sobre a participação de atletas trans no esporte, com base no programa Esporte Espetacular. Metodologicamente, a pesquisa caracteriza-se como qualitativa e exploratória, utilizando a etnografia de tela como ferramenta de produção dos dados. Foram analisadas 4 reportagens produzidas de 2017 a 2019, totalizando aproximadamente 35 minutos. Os achados permitem inferir que todas as narrativas televisivas apresentam equívocos conceituais e uma abordagem que tende ao sensacionalismo, além de enfocarem um tipo específico de identidade transgênero, no discurso biomédico da vantagem e da testosterona e de episódios de negação, constrangimento e não-reconhecimento do corpo trans no Esporte.


Resumen En la actualidad, el escenario deportivo, el escenario deportivo, tal como es retratado por los medios de comunicación, presenta nuevas perspectivas con respecto a las prácticas atlético-deportivas LGBTI+. El objetivo de esta investigación fue analizar la cobertura televisiva sobre la participación de atletas trans en el deporte, basándose en el programa "Esporte Espetacular". La metodología empleada en esta investigación se caracteriza como cualitativa y exploratoria, y utiliza la Etnografía de Pantalla como herramienta para la producción de datos.. Se analizaron 4 reportajes, producidos desde 2017 hasta 2019, con una duración total de aproximadamente 35 minutos. Los hallazgos sugieren que todas las narrativas televisivas analizadas presentan errores conceptuales y muestran un enfoque que tiende hacia el sensacionalismo, centrándose en un tipo particular de identidad transgénero, en el discurso biomédico de la ventaja y la testosterona, así como en episodios de negación, vergüenza y no reconocimiento del cuerpo trans en el deporte.


Abstract Nowadays, the sports scenario, particularly portrayed by the media, is presenting new perspectives regarding LGBTI+ athletic-sport practices. The objective of this research was to analyze television coverage of the participation of transgender athletes in sports, based on the program "Esporte Espetacular". Methodologically, the research is characterized as qualitative and exploratory, using screen ethnography as a data production tool. Four reports produced from 2017 to 2019 were analyzed, totaling approximately 35 minutes. The findings allow us to infer that all episodes' narratives present conceptual mistakes and an approach that tends towards sensationalism, focusing on a particular type of transgender identity, on the biomedical discourse of advantage and testosterone, as well as instances of denial, embarrassment, and non-recognition of the transgender body in sports.

20.
Ginecol. obstet. Méx ; Ginecol. obstet. Méx;91(3): 197-209, ene. 2023. tab, graf
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1448333

RESUMEN

Resumen ANTECEDENTES: El síndrome de Hamman se caracteriza por la coexistencia de aire libre en el mediastino sin una causa identificable; también puede ser secundario a traumatismos, infecciones intratorácicas, procedimientos médicos como la esofagoscopia y broncoscopia. Su incidencia se estima entre 1 en 2000 y 1 en 100,000 embarazos. CASO CLÍNICO: Paciente de 18 años, con 65 kg de peso, 1.56 m de talla e IMC 26.74, correspondiente a sobrepeso, primigesta, sin antecedentes patológicos ni heredofamiliares relevantes, con evolución normal del embarazo. A las 39 semanas acudió a urgencias ginecológicas debido a contractilidad uterina y salida de líquido por la vagina. La finalización del embarazo fue por parto, con recién nacido vivo. En el puerperio mediato (30 horas después del parto) súbitamente tuvo disnea, ortopnea y dolor en la región infraclavicular, sensación de "burbujeo" en la parte anterior del tórax. La radiografía simple de tórax mostró aire libre en el mediastino y enfisema subcutáneo. La TAC de tórax evidenció múltiples burbujas de aire, con extensión del espacio perivertebral de predominio derecho. La conclusión diagnóstica fue: enfisema extenso subcutáneo en los espacios del cuello, con alcance al mediastino anterior, con extenso neumomediastino y cardiomegalia global. CONCLUSIONES: El síndrome de Hamman prevalece en primigestas jóvenes y tiene un curso benigno. El tratamiento debe ser conservador, con oxígeno y analgésicos.


Abstract BACKGROUND: Hamman's syndrome is characterized by the coexistence of free air in the mediastinum without an identifiable cause; it may also be secondary to trauma, intrathoracic infections, medical procedures such as oesophagoscopy and bronchoscopy. Its incidence is estimated to be between 1 in 2000 and 1 in 100,000 pregnancies. CLINICAL CASE: 18-year-old female patient, weight 65 kg, height 1.56 m and BMI 26.74, corresponding to overweight, primigravida, with no relevant pathological or heredofamilial history, with normal evolution of pregnancy. At 39 weeks, she attended the gynaecological emergency department due to uterine contractility and leakage of fluid from the vagina. The pregnancy was terminated by delivery, with a live newborn. In the immediate postpartum period (30 hours after delivery) she suddenly experienced dyspnoea, orthopnoea and pain in the infraclavicular region, with a sensation of "bubbling" in the anterior chest. Plain chest X-ray showed free air in the mediastinum and subcutaneous emphysema. Chest CT showed multiple air bubbles, with extension of the perivertebral space predominantly on the right. The diagnostic conclusion was: extensive subcutaneous emphysema in the neck spaces extending into the anterior mediastinum, with extensive pneumomediastinum and global cardiomegaly. CONCLUSIONS: Hamman syndrome is prevalent in young primigravidae and has a benign course. Treatment should be conservative, with oxygen and analgesics.

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