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The Brain Bee serves as an international platform providing high school students with opportunities for undergraduate and graduate-level education in neuroscience. This annual competition welcomes participation from high school students and schools, fostering both individual- and team-based competition in a dynamic and engaging manner. The assessment involves a primary multiple-choice and short-answer exam, a secondary neuroanatomical lab practical exam, and a final oral exam administered by affiliated faculty or coordinators. During the final oral exam, the top ten students are given three chances and simultaneously respond to the same question. They write their answers on individual whiteboards until only one student remains. This unique format not only adds an element of excitement, but also allows students to build confidence within themselves and connections within STEM fields. The importance of local Brain Bees is emphasized as winners from these chapters earn the opportunity to compete nationally, and potentially internationally. Throughout the academic year, local Brain Bees, led by college students and faculty, educate high school students about various neuroscientific fields and their STEM applications through interactive and engaging sessions. These sessions culminate in an annual competition where students explore the intricacies of the human brain, spanning human physiology, pathology, and behavior. The program also exposes students to anatomical models, research, and guest speakers within the field of neuroscience. To ensure the success and continuity of local chapters, our 12-tips article provides valuable advice for running a safe and smoothly operating Brain Bee program, promoting sustained participation and enthusiasm.

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Soldiers of the Mexican Army with obesity were subjected to an intense 60-day weight-loss course consisting of a controlled diet, daily physical training, and psychological sessions. The nutritional treatment followed the European Society of Cardiology (ESC) recommendations, incorporating elements of the traditional milpa diet in the nutritional intervention. The total energy intake was reduced by 200 kcal every 20 days, starting with 1,800 kcal and ending with 1,400 kcal daily. On average, the participants reduced their body weight by 18 kg. We employed an innovative approach to monitor the progress of the twelve soldiers who completed the entire program. We compared the untargeted metabolomics profiles of their urine samples, taken before and after the course. The data obtained through liquid chromatography and high-resolution mass spectrometry (LC-MS) provided insightful results. Classification models perfectly separated the profiles pre and post-course, indicating a significant reprogramming of the participants' metabolism. The changes were observed in the C1-, vitamin, amino acid, and energy metabolism pathways, primarily affecting the liver, biliary system, and mitochondria. This study not only demonstrates the potential of rapid weight loss and metabolic pathway modification but also introduces a non-invasive method for monitoring the metabolic state of individuals through urine mass spectrometry data.

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En la provincia Pinar del Río, en los últimos cinco años se ha notado cierto descenso de los rendimientos de los corredores de distancias cortas. Entre uno de los aspectos que incide en ello figura el pobre aprovechamiento de la arrancada. Esta investigación se realizó con el objetivo de analizar del comportamiento, en la arrancada de la carrera de 100 metros planos de los velocistas escolares categoría 12-15 años de la Escuela de Iniciación Deportiva de Pinar del Río. En la actualidad, se procura personalizar el tipo de arrancada a las características específicas de cada atleta, local para garantizar un aprovechamiento óptimo de sus características antropométricas, potencia muscular, distancia de carrera y ubicación en la pista. El estudio se llevó a cabo con los ocho atletas de la categoría 12-15 años, sexo masculino, matriculados en el área de velocidad en la Escuela de Iniciación Deportiva de Pinar del Río. Se realizó una revisión documental del proceso de entrenamiento de la arrancada baja que se lleva a cabo en los velocistas escolares, además de aplicar otros métodos empíricos como: la observación a los entrenamientos, la medición y la técnica de encuesta. Los resultados arrojados muestran que existen factores en el proceso de entrenamiento que atentan contra el éxito competitivo de estos atletas; esto genera necesidad de organizar acciones en un orden secuencial, lógica que permitan su perfeccionamiento en base al ajuste a los cambios de la condición de estos sujetos.

Na província de Pinar del Río, nos últimos cinco anos, notou-se uma certa diminuição no desempenho dos corredores de curta distância. Um dos aspectos que afeta isso é o mau aproveitamento da largada. Esta pesquisa foi realizada com o objetivo de analisar o comportamento na largada da corrida de 100 metros rasos de velocistas escolares da categoria 12-15 anos da Escola de Iniciação Esportiva Pinar del Río. Atualmente, são feitos esforços para personalizar o tipo de largada às características específicas de cada atleta local para garantir o aproveitamento ideal de suas características antropométricas, potência muscular, distância de corrida e localização na pista. O estudo foi realizado com oito atletas da categoria 12 a 15 anos, do sexo masculino, matriculados na área de velocidade da Escola de Iniciação Esportiva Pinar del Río. Foi realizada uma revisão documental do processo de treinamento low start realizado em velocistas escolares, além de aplicar outros métodos empíricos como: observação ao treinamento, medição e técnica de levantamento. Os resultados mostram que existem fatores no processo de treinamento que ameaçam o sucesso competitivo desses atletas; Isso gera a necessidade de organizar as ações em uma ordem sequencial e lógica que permita seu aprimoramento a partir do ajuste às mudanças na condição desses sujeitos.

SUMMARY In the Pinar del Río province, in the last five years a certain decrease in the performance of short distance runners has been noted. One of the aspects that affects this is the poor use of the start. This research was carried out with the objective of analyzing the behavior at the start of the 100-meter dash race of school sprinters in the 12-15-year category of the Pinar del Río Sports Initiation School. Currently, efforts are made to personalize the type of start to the specific characteristics of each local athlete to guarantee optimal use of their anthropometric characteristics, muscular power, race distance and location on the track. The study was carried out with eight athletes in the 12-15-year-old category, male, enrolled in the speed area at the Pinar del Río Sports Initiation School. A documentary review of the low start training process carried out in school sprinters was carried out, in addition to applying other empirical methods such as: observation to training, measurement and survey technique. The results show that there are factors in the training process that threaten the competitive success of these athletes; this generates the need to organize actions in a sequential, logical order that allows their improvement based on adjustment to changes in the condition of these subjects.

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Research background: Soursop nectar contains antioxidants and is preserved by pasteurization. However, this technology impairs its physicochemical properties and bioactive compounds. An alternative is therefore thermoultrasound, which could counteract these effects. The thermosonicated nectar was compared with a pasteurized one and the in vitro bioaccessibility of antioxidants was estimated. Experimental approach: The soursop nectar (25 %) was processed and the response surface methodology was used to determine the optimal conditions for thermoultrasound treatment (TUS). The TUS (75-90 % amplitude, 3.15-15 min) was applied, and 2 % stevia and 6 % agave inulin were added as sweeteners. The microbiological, physicochemical, enzymatic and antioxidant properties were analyzed. The properties of thermosonicated nectar obtained under optimal conditions were compared with pasteurized nectar. In addition to the above determinations, microstructure, total dietary fiber (TDF) and in vitro bioaccessibility of antioxidants were determined. Results and conclusions: The response variables that fit the mathematical model were L*, b*, chroma (C*), total phenolic content (TPC) and antioxidant activity determined by ABTS•+, DPPH˙ and Fe(III) reducing antioxidant power (FRAP). The L* and DPPH˙ were affected by quadratic time and TPC by time (p<0.0001). The optimum TUS condition was 82 % amplitude for 9.15 min and the responses variables were L*, b* and C* (45.48, 3.55 and 3.62, respectively), TPC expressed as gallic acid equivalents (38.40 mg/100 mL), ABTS•+ expressed as Trolox equivalents (TE) (31.28 µmol/100 mL), DPPH˙ expressed as TE (124.22 µmol/100 mL) and FRAP expressed as Fe(II) (3.06 µmol/100 mL). Compared to the pasteurized sample, thermosonicated sample had high values of L* (45.56), h° (-56.49), TPC (26.63 mg/100 mL), ABTS•+ and DPPH˙ (22.03 and 129.22 µmol/100 mL, respectively), FRAP (3.10 µmol/100 mL) and low pectin methylesterase (PME) activity (0.28 U/mL). For in vitro bioaccessibility, thermosonicated nectar showed high absorption of TPC (15.26/100 mL) and high antioxidant activity determined by ABTS (34.92 µmol/100 mL) and FRAP (7.88 µmol/100 mL). Novelty and scientific contribution: The thermoultrasound improves the physicochemical properties and in vitro bioaccessibility of antioxidants in soursop nectar. On the other hand, as an alternative, this beverage offers low-calorie alternative with prebiotic properties that benefits consumer health.

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RESUMEN Agave salmiana es una subespecie originaria de México, ha sido participe en distintos periodos históricos, utilizado en la construcción, vestimenta, alimento y bebida. Destacando el aguamiel como subproducto, cuya particularidad es su alto contenido de azúcares (fructooligosacáridos). Algunos subproductos derivan del aguamiel, como el pulque y el jarabe de agave; el primero resultado de la fermentación y el segundo de la concentración de solutos mediante tratamientos térmicos y/o enzimáticos. Por lo anterior, el objetivo de esta revisión fue recopilar información de los últimos años sobre Agave salmiana y subproductos de interés nutricional y funcional, así como su uso en productos alimenticios, para lo cual se usaron las bases de datos: Medline/PubMed, SciELO, ELSEVIER, así como Normas Oficiales Mexicanas y páginas web privadas. La estrategia de búsqueda se realizó combinando el uso de operadores booleanos "AND" y "OR", utilizando las siguientes palabras: aguamiel and characterization, pulque and microorganisms or fermentation, agave syrup and bread. Esta revisión concluye que el consumo, de manera controlada, de los subproductos de Agave salmiana, trae consigo múltiples beneficios a la salud, principalmente al fortalecer la microbiota intestinal, por lo que pueden ser incluidos en la dieta habitual de una persona sana, además, pueden ser utilizados como parte del tratamiento nutricional, en bajas cantidades, en patologías como diabetes tipo 2 e intolerancia a la insulina, colitis ulcerosa y cáncer de colon siempre con supervisión médica. Por otra parte, aún existe la necesidad de generar más información sobre el tema a través de investigación científica.

ABSTRACT Agave salmiana is a native subspecies from Mexico, it has been part of different historical periods, used in construction, clothing, food and drink; highlighting the aguamiel as a by-product, whose particularity is its high sugar content (fructooligosaccharides). Some by-products are derived from aguamiel, such as pulque and agave syrup; the first is the result of fermentation and the second of the concentration of solutes by thermal and/or enzymatic treatments. Therefore, the objective of this review was to collect recent information about Agave salmiana and by-products of nutritional and functional interest, as well as use in food products. We used the following databases were used: Medline/PubMed, SciELO, ELSEVIER, as well as the Official Mexican Standards and private web pages. The search strategy was carried out with the boolean operators "AND" and "OR", using the following words: aguamiel and characterization, pulque and microorganisms or fermentation, agave syrup and bread. This review concludes that the consumption, in a controlled manner, of the by-products of Agave salmiana, brings with it multiple health benefits, mainly by strengthening the intestinal microbiota, so they can be included in the usual diet of a healthy person. In addition, by-products can be used as part of nutritional treatments, in low amounts, for diseases such as type 2 diabetes and insulin intolerance, ulcerative colitis and colon cancer, always with medical supervision. On the other hand, there is still a need to generate more information about the subject through scientific research.

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ABSTRACT The objective of this work was to evaluate the proximal chemical composition, antioxidant activity assays with total phenol, 2,2-Diphenyl-1-Picrylhydrazyl, 2'2-azino-bis(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt, iron reducing antioxidant power, and the fatty acid composition of fermented and dried cocoa beans, roasted cocoa beans and cocoa pulp bar. The fermented and dried cocoa beans were purchased, roasted (115 °C for 5 min) and homogenized. The mixture was brought to its melting point (58 °C for 5 min). In the cocoa pulp bar there was an increase in lipids (53.0%). The roasted affected the concentration of ashes (3.38-2.98%). Total phenol (1.8, 1.5 and 1.3 mg AEG kg-1), 2,2-Diphenyl-1-Picrylhydrazyl (65.8, 64.4 and 65.0 μmol ET kg-1), 2'2-azino-bis(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (3.60, 3.00 and 2.30 μmol EAC kg-1) and iron reducing antioxidant power (0.09, 0.07 and 0.06 mg Fe2+ kg-1) presented significant differences (p<0.05) in the fermented and dried cocoa beans, roasted cocoa beans and cocoa pulp bar respectively, however, the changes were minimal. The cocoa pulp bar had a higher concentration of unsaturated fatty acid (89.2%), where oleic (48.6%) was predominated. Therefore, the cocoa pulp bar is a food with an important antioxidant content, moreover, it has a higher concentration of unsaturated fatty acid, which is desirable in the diet.

RESUMEN El objetivo de este trabajo fue evaluar la composición química proximal, el contenido de antioxidantes con los ensayos de fenoles totales, 2,2-Difenil-1-Picrilhidrazil, Ácido 2'2-azino-bis (3-etilbenzotiazolin-6-sulfónico), sal de diamonio y poder antioxidante reductor del hierro, y la composición de ácidos grasos en los granos de cacao fermentados y secos, granos de cacao tostados y barra de pulpa de cacao. Se compraron los granos de cacao fermentado y seco y se tostaron (115 °C durante 5 min), y se homogeneizaron. La mezcla se llevó a su punto de fusión (58 °C durante 5 min). En la barra de pulpa de cacao hubo un aumento de lípidos (53,0%). El tostado afectó la concentración de cenizas (3,38 - 2,98%). En los resultados de fenoles totales (1,80, 1,50 y 1,30 mg AEG kg-1), 2,2-Difenil-1-Picrilhidrazil (65,8, 64,4 y 65,0 μmol ET kg-1), Ácido 2'2-azino-bis (3-etilbenzotiazolin-6-sulfónico), sal de diamonio (3,6, 3,0 y 2,3 μmol EAC kg-1) y poder antioxidante reductor del hierro (0.09, 0.07 y 0.06 mg Fe2+ kg-1) presentaron diferencias significativas (p<0.05) en los granos de cacao fermentados y secos, granos de cacao tostados y barra de pulpa de cacao respectivamente, sin embargo, los cambios fueron mínimos. La barra de pulpa de cacao presentó una mayor concentración de ácidos grasos insaturados (89%), donde predominó el oleico (48,6%). Por lo tanto, la barra de pulpa de cacao es un alimento con importante contenido en antioxidantes, además, tiene una mayor concentración de ácidos grasos insaturados, lo que es deseable en la dieta.

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The consumption of vegetables in Mexico includes a wide variety of plants that grow naturally as weeds in the fields. The intake of these vegetables is very important in the Mexican diet because these plants supply an important input of nutrients and compounds such as fiber, vitamins, minerals, and antioxidants. Thus, the plants may be universally promoted as healthy. However, there is little information about these vegetables of popular consumption, especially in terms of the nutritional changes caused by boiling. To determine the influence of boiling on five plants of popular consumption in Mexico, the nutritional composition (proximal analysis, dietary fiber, and oxalates), antioxidant compounds (ascorbic acid, phenolics), antioxidant activity (measured by ABTS and DPPH assays), and physicochemical characteristics (water retention capacity, viscosity, color, and SEM) were evaluated. The boiling affected the nutritional composition of plants, mainly soluble compounds as carbohydrates (sugars and soluble fiber), ash, ascorbic acid, and phenolic compounds and caused changes in food hydration and color. Therefore, it is recommended that these plants be consumed raw or with short boiling times and included the cooking water in other preparations to take advantage of the nutrients released in the food matrix. In the future, to complete studies, 3 to 5 min of cooking should be considered to minimize undesirable modifications in terms of the vegetables' composition.

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Resumen Para el proceso de control efectivo de la resistencia, en las disciplinas cíclicas, se ha sugerido la determinación de índices que tengan en cuenta el desarrollo de esta capacidad respecto a la velocidad. Este es uno de los factores fundamentales que incide en su manifestación y que hacen que el nivel de información, sobre este proceso, dependa del resultado de las pruebas utilizadas. Se consideran, además, las particularidades de estas disciplinas deportivas, la edad y nivel de los deportistas. Se desarrolla esta investigación con el objetivo de proponer una prueba de velocidad máxima específica para la marcha deportiva, acorde a las particularidades de las edades entre 12 y 13 años, basada en la carencia de referencias sobre pruebas específicas de velocidad para los marchistas escolares. Esta posee una metodología de aplicación y procesamiento para la determinación del coeficiente de resistencia. Para ello se emplearon métodos del nivel teórico (análisis y síntesis, inducción deducción) y empírico (medición, análisis de documentos y matemáticos estadísticos). Se parte de una población conformada por los 8 atletas de la Escuela de Iniciación Deportiva Escolar (EIDE) Ormani Arenado de Pinar del Río, cuatro de ellos del sexo femenino y a 10 entrenadores como fuente de información. Como propuesta, la prueba de marcha sobre 100 m, se observa el cumplimiento de las exigencias del reglamento competitivo a la correcta ejecución técnica.

Abstract For the process of effective control of endurance in cyclical disciplines, it has been suggested to determine parameters which consider the development of this capacity according to speed. This is one of the fundamental factors that influences its manifestation and that makes the level of information about this process depend on the result of the tests used. In addition to the particularities of these sports, the age and level of the athletes are also considered. This research is developed with the aim of proposing a specific maximum speed test for sport walking, according to the particularities of the ages between 12 and 13 years, based on the lack of references on specific speed tests for school walkers. It has an application and processing methodology for the determination of the endurance coefficient. The methods used were theoretical (analysis and synthesis, induction - deduction) and empirical (measurement, document analysis and statistical mathematics). It is started from a population made up of 8 athletes from "Ormani Arenado" Initiation Sport School (EIDE) in Pinar del Río, four of them women and 10 coaches as a source of information. As a proposal, in the 100 m sport walking test, compliance with the requirements of the competitive regulations to the correct technical execution is observed.

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The aim of this research was to determine the physicochemical properties, microbial counts and aflatoxin M1 (AFM1) levels of thermoultrasonicated, pasteurized and untreated milk (control) at days 1, 7 and 14 of storage. Thermoultrasound treatment was performed at a rate of 20 kHz for 10 or 15 min and 95% amplitude on homogenized and non-homogenized milk samples. Results showed that most physicochemical parameters were within the Mexican norms established for milk. Ultrasound treatment for 15 min reduced solids precipitation (p < 0.05) in unhomogenized milk during storage as compared to the pasteurized milk. All samples complied with aerobic mesophilic counts limits set by the Mexican norm except the control and the homogenized milk sample which was thermoultrasonicated for 10 min. Enterobacteriaceae counts of pasteurized and 15 min-thermoultrasound homogenized milks complied with the norm. The lowest levels of AFM1 were found in the 10 min-thermoultrasound unhomogenized milk (0.15 ±â€¯0.05 pg AFM⁠1E/mL) one day after storage. Thermoultrasound did not affect the color of samples but homogenized milk treated for 10 min exhibited less total color difference. A high phenolic content was found in thermoultrasound and pasteurized milks on day 1. Thermoultrasound could be an alternative to milk pasteurization that preserves the physicochemical and microbiological quality of milk while reducing AFM1 levels.

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The aim of this study was to evaluate the stability of color, betaxanthin, and betacyanin pigments in the presence of Cu(II)-dependent hydroxyl radicals (HO•) from ultrasonicated purple cactus pear juice at amplitudes of 40%, 60%, and 80%, in comparison to untreated sample. L* parameter of juice treated at 40% and 80% amplitude for 25 and 15 min, respectively (11.3 and 9.3, respectively), were significantly higher compared to the control; b* and hue parameters of juice treated at 80%, 25 min showed values of 1.7 and 0.1, respectively. Color differences (ΔE) were lower (<3) for juices treated at high amplitude (80%) and short times (3-5 min). Juice treated at 40% 15 min, 60% 25 min, 80% 15 and 25 min presented high values of betacyanins (281.7 mg·L-1, 255.9 mg·L-1, 294.4 mg·L-1, and 276.7 mg·L-1, respectively). Betaxanthin values were higher in the juices treated at 40% 5 min and 80% 15 and 25 min (154.2 mg·L-1, 135.2 mg·L-1, and 128.5 mg·L-1, respectively). Purple cactus pear juice exhibited significant chelating activity of copper ions and great stability when exposed to HO•.

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The UDP-glucose pyrophosphorylase of Streptococcus pneumoniae (GalUSpn) is absolutely required for the biosynthesis of capsular polysaccharide, the sine qua non virulence factor of pneumococcus. Since the eukaryotic enzymes are completely unrelated to their prokaryotic counterparts, we propose that the GalU enzyme is a critical target to fight the pneumococcal disease. A recombinant GalUSpn was overexpressed and purified. An enzymatic assay that is rapid, sensitive and easy to perform was developed. This assay was appropriate for screening chemical libraries for searching GalU inhibitors. This work represents a fundamental step in the exploration of novel antipneumococcal drugs.

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The purpose of this research was to optimize the thermoultrasound conditions for blackberry juice using the response surface methodology and considering juice quality parameters and antioxidant capacity. With the exception of microbial growth, the response variables showed high correlation coefficients with the mathematical model (R2adj>0.91). Thermoultrasound treatment inactivated all the evaluated microorganisms, and at the optimum conditions (50±1°C at 17±1min) it increased enzyme inactivation and antioxidant activity in comparison to pasteurized juice. The results demonstrated that thermoultrasound can be an alternative to pasteurization for the production of safe and high-quality juices with the added value of higher concentration of bioactive compounds and antioxidant capacity.

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Blackberry processing generates up to 20% of residues composed mainly of peel, seeds and pulp that are abundant in flavonoids. The objective of this study was to optimize the ultrasound conditions, in a closed system, for antioxidants extraction, using the response surface methodology. Blackberry (Rubus fructicosus) residues were analyzed for total phenolics, total anthocyanins, and antioxidant activity by ABTS and DPPH. The selected independent variables were ultrasound amplitude (X1: 80%-90%) and extraction time (X2: 10-15 min), and results were compared with conventional extraction methods. The optimal conditions for antioxidants extraction were 91% amplitude for 15 min. The results for total phenolic content and anthocyanins and antioxidant activity by ABTS and DPPH were of 1201.23 mg gallic acid equivalent (GAE)/100 g dry weight basis (dw); 379.12 mg/100 g·dw; 6318.98 µmol Trolox equivalent (TE)/100 g·dw and 9617.22 µmol TE/100 g·dw, respectively. Compared to solvent extraction methods (water and ethanol), ultrasound achieved higher extraction of all compounds except for anthocyanins. The results obtained demonstrated that ultrasound is an alternative to improve extraction yield of antioxidants from fruit residues such as blackberry.

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A new hybrid Multiphase Simulated Annealing Algorithm using Boltzmann and Bose-Einstein distributions (MPSABBE) is proposed. MPSABBE was designed for solving the Protein Folding Problem (PFP) instances. This new approach has four phases: (i) Multiquenching Phase (MQP), (ii) Boltzmann Annealing Phase (BAP), (iii) Bose-Einstein Annealing Phase (BEAP), and (iv) Dynamical Equilibrium Phase (DEP). BAP and BEAP are simulated annealing searching procedures based on Boltzmann and Bose-Einstein distributions, respectively. DEP is also a simulated annealing search procedure, which is applied at the final temperature of the fourth phase, which can be seen as a second Bose-Einstein phase. MQP is a search process that ranges from extremely high to high temperatures, applying a very fast cooling process, and is not very restrictive to accept new solutions. However, BAP and BEAP range from high to low and from low to very low temperatures, respectively. They are more restrictive for accepting new solutions. DEP uses a particular heuristic to detect the stochastic equilibrium by applying a least squares method during its execution. MPSABBE parameters are tuned with an analytical method, which considers the maximal and minimal deterioration of problem instances. MPSABBE was tested with several instances of PFP, showing that the use of both distributions is better than using only the Boltzmann distribution on the classical SA.

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The objective of this study was to evaluate changes in color, betalain content, browning index, viscosity, physical stability, microbiological growth, antioxidant content and antioxidant activity of purple cactus pear juice during storage after thermoultrasonication at 80% amplitude level for 15 and 25 min in comparison with pasteurized juice. Thermoultrasound treatment for 25 min increased color stability and viscosity compared to treatment for 15 min (6.83 and 6.72 MPa, respectively), but this last parameter was significantly lower (p<0.05) compared to the control and pasteurized juices (22.47 and 26.32 MPa, respectively). Experimental treatment reduced significantly (p<0.05) sediment solids in juices. Total plate counts decreased from the first day of storage exhibiting values of 1.38 and 1.43 logCFU/mL, for 15 and 25 min treatment, respectively. Compared to the control, both treatments reduced enterobacteria counts (1.54 logCFU/mL), and compared to pasteurized juice decreased pectinmethylesterase activity (3.76 and 3.82 UPE/mL), maintained high values of ascorbic acid (252.05 and 257.18 mg AA/L) and antioxidant activity (by ABTS: 124.8 and 115.6 mg VCEAC/100 mL; and DPPH: 3114.2 and 2757.1 µmol TE/L). During storage thermoultrasonicated juices had a minimum increase in pectinmethylesterase activity (from day 14), and exhibited similar total plate counts to pasteurized juice. An increase of phenolic content was observed after 14 days of storage, particularly for treatment at 80%, 25 min, and an increase in antioxidant activity (ABTS, DPPH) by the end of storage.

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El aneurisma de la arteria subclavia es una entidad extremadamente rara que no sobrepasa el 1 por ciento del total de los aneurismas periféricos y pueden situarse en diferentes sectores de la arteria. El objetivo de este trabajo es describir el diagnóstico y tratamiento empleado en un paciente portador de un aneurisma verdadero del tronco tirocervical derecho, rama de la arteria subclavia, considerado como infrecuente. Se trata de una paciente femenina de 48 años de edad, con antecedentes de aumento de volumen en la zona supraclavicular derecha, tumoración que late y se expande sin trauma previo en dicha zona. Las manifestaciones clínicas consistieron en dolor y parestesias que se irradiaban al miembro superior derecho. A través de estudios ultrasonográficos (ecodoppler y angioTac) se le diagnosticó un aneurisma del tronco tirocervical derecho, rama de la primera porción de la arteria subclavia. Fue intervenida quirúrgicamente en el Instituto Nacional de Angiología y Cirugía Vascular. Se realizó una aneurismectomía y ligadura proximal y distal utilizando un abordaje supraclavicular. Se encontró un aneurisma verdadero de la arteria señalada. Los estudios ultrasonográficos permitieron un diagnóstico certero del problema. La técnica quirúrgica utilizada fue exitosa y la paciente mostró una evolución satisfactoria, sin complicaciones hasta el momento actual con regresión de sus síntomas clínicos(AU)

The subclavian artery aneurysm is an extremely rare disease that barely accounts for 1 percent of all peripheral aneurysms and may be located in different sectors of the aorta. The objective of this paper was to describe the diagnosis and treatment used in a patient carrying an unusual true aneurysm of the right thyrocervical trunk, the subclavian artery branch. The case of 48 years-old woman, with a history of raised volume of the right supraclavicular area, beating tumor that expands, and no previous trauma in the area. The clinical symptoms were pain and parestesias irradiating to the right upper member. Through the ultrasonographic studies (Ecodoppler and AngioTac), she was diagnosed a right thyrocervical trunk aneurysm, branch of the first portion of the subclavian artery. She was operated on at the National Institute of Angiology and Vascular Surgery. She underwent aneurysmectomy and proximal and distal ligature by using supraclavicular approach. A true aneurysm of the referred artery was found. The ultrasonographic studies allowed a precise diagnosis of the problem and the surgical procedure was successful. The patient recovered satisfactorily with no complications up to the present and regression of her clinical symptoms(AU)

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El aneurisma de la arteria subclavia es una entidad extremadamente rara que no sobrepasa el 1 por ciento del total de los aneurismas periféricos y pueden situarse en diferentes sectores de la arteria. El objetivo de este trabajo es describir el diagnóstico y tratamiento empleado en un paciente portador de un aneurisma verdadero del tronco tirocervical derecho, rama de la arteria subclavia, considerado como infrecuente. Se trata de una paciente femenina de 48 años de edad, con antecedentes de aumento de volumen en la zona supraclavicular derecha, tumoración que late y se expande sin trauma previo en dicha zona. Las manifestaciones clínicas consistieron en dolor y parestesias que se irradiaban al miembro superior derecho. A través de estudios ultrasonográficos (ecodoppler y angioTac) se le diagnosticó un aneurisma del tronco tirocervical derecho, rama de la primera porción de la arteria subclavia. Fue intervenida quirúrgicamente en el Instituto Nacional de Angiología y Cirugía Vascular. Se realizó una aneurismectomía y ligadura proximal y distal utilizando un abordaje supraclavicular. Se encontró un aneurisma verdadero de la arteria señalada. Los estudios ultrasonográficos permitieron un diagnóstico certero del problema. La técnica quirúrgica utilizada fue exitosa y la paciente mostró una evolución satisfactoria, sin complicaciones hasta el momento actual con regresión de sus síntomas clínicos(AU)

The subclavian artery aneurysm is an extremely rare disease that barely accounts for 1 percent of all peripheral aneurysms and may be located in different sectors of the aorta. The objective of this paper was to describe the diagnosis and treatment used in a patient carrying an unusual true aneurysm of the right thyrocervical trunk, the subclavian artery branch. The case of 48 years-old woman, with a history of raised volume of the right supraclavicular area, beating tumor that expands, and no previous trauma in the area. The clinical symptoms were pain and parestesias irradiating to the right upper member. Through the ultrasonographic studies (Ecodoppler and AngioTac), she was diagnosed a right thyrocervical trunk aneurysm, branch of the first portion of the subclavian artery. She was operated on at the National Institute of Angiology and Vascular Surgery. She underwent aneurysmectomy and proximal and distal ligature by using supraclavicular approach. A true aneurysm of the referred artery was found. The ultrasonographic studies allowed a precise diagnosis of the problem and the surgical procedure was successful. The patient recovered satisfactorily with no complications up to the present and regression of her clinical symptoms(AU)

RESUMEN

El aneurisma de la arteria subclavia es una entidad extremadamente rara que no sobrepasa el 1 % del total de los aneurismas periféricos y pueden situarse en diferentes sectores de la arteria. El objetivo de este trabajo es describir el diagnóstico y tratamiento empleado en un paciente portador de un aneurisma verdadero del tronco tirocervical derecho, rama de la arteria subclavia, considerado como infrecuente. Se trata de una paciente femenina de 48 años de edad, con antecedentes de aumento de volumen en la zona supraclavicular derecha, tumoración que late y se expande sin trauma previo en dicha zona. Las manifestaciones clínicas consistieron en dolor y parestesias que se irradiaban al miembro superior derecho. A través de estudios ultrasonográficos (ecodoppler y angioTac) se le diagnosticó un aneurisma del tronco tirocervical derecho, rama de la primera porción de la arteria subclavia. Fue intervenida quirúrgicamente en el Instituto Nacional de Angiología y Cirugía Vascular. Se realizó una aneurismectomía y ligadura proximal y distal utilizando un abordaje supraclavicular. Se encontró un aneurisma verdadero de la arteria señalada. Los estudios ultrasonográficos permitieron un diagnóstico certero del problema. La técnica quirúrgica utilizada fue exitosa y la paciente mostró una evolución satisfactoria, sin complicaciones hasta el momento actual con regresión de sus síntomas clínicos(AU)

The subclavian artery aneurysm is an extremely rare disease that barely accounts for 1 % of all peripheral aneurysms and may be located in different sectors of the aorta. The objective of this paper was to describe the diagnosis and treatment used in a patient carrying an unusual true aneurysm of the right thyrocervical trunk, the subclavian artery branch. The case of 48 years-old woman, with a history of raised volume of the right supraclavicular area, beating tumor that expands, and no previous trauma in the area. The clinical symptoms were pain and parestesias irradiating to the right upper member. Through the ultrasonographic studies (Ecodoppler and AngioTac), she was diagnosed a right thyrocervical trunk aneurysm, branch of the first portion of the subclavian artery. She was operated on at the National Institute of Angiology and Vascular Surgery. She underwent aneurysmectomy and proximal and distal ligature by using supraclavicular approach. A true aneurysm of the referred artery was found. The ultrasonographic studies allowed a precise diagnosis of the problem and the surgical procedure was successful. The patient recovered satisfactorily with no complications up to the present and regression of her clinical symptoms(AU)

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RESUMEN

The Chaotic Multiquenching Annealing algorithm (CMQA) is proposed. CMQA is a new algorithm, which is applied to protein folding problem (PFP). This algorithm is divided into three phases: (i) multiquenching phase (MQP), (ii) annealing phase (AP), and (iii) dynamical equilibrium phase (DEP). MQP enforces several stages of quick quenching processes that include chaotic functions. The chaotic functions can increase the exploration potential of solutions space of PFP. AP phase implements a simulated annealing algorithm (SA) with an exponential cooling function. MQP and AP are delimited by different ranges of temperatures; MQP is applied for a range of temperatures which goes from extremely high values to very high values; AP searches for solutions in a range of temperatures from high values to extremely low values. DEP phase finds the equilibrium in a dynamic way by applying least squares method. CMQA is tested with several instances of PFP.

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