RESUMEN
Surface lipids in plants include cutin, cuticular wax and suberin. sn-Glycerol-3-phosphate acyltransferases (GPATs) facilitate the acylation of sn-glycerol-3-phosphate (G3P) utilizing a fatty acyl group from acyl-coenzyme A (acyl-CoA) or acyl-acyl carrier protein (acyl-ACP) as substrates for the biosynthesis of plant extracellular lipids such as suberin and cutin. Here we found that Arabidopsis GPAT4 and GPAT8 are specifically expressed in endodermis cells of roots where suberin was accumulated. GPAT4 mutation significantly decreased the amounts of the C16 and C18 ω-oxidized suberin monomers, whereas the mutation of GPAT8 had little effect on the suberin production, and the functions of both were not redundant. Root suberin phenotype analysis of gpat4-1 and gpat6-1 single or double mutant revealed that GPAT4 and GPAT6 play redundant functions. Interestingly, the gpat4-1 gpat8-1 double mutant displayed a glossy stem phenotype since fewer wax crystals were accumulated. This phenotype was not shown in either parent. Further study showed that the amounts of most wax components were significantly decreased. Taken together, our findings revealed that GPAT4 has an additive effect with GPAT6 in the root suberin biosynthesis, and plays a redundant role in wax production with GPAT8.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Lípidos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Glicerol/química , Glicerol/metabolismo , Fosfatos/metabolismo , Plantas/metabolismoRESUMEN
Genetic signature of climate adaptation has been widely recognized across the genome of many organisms; however, the eco-physiological basis for linking genomic polymorphisms with local adaptations remains largely unexplored. Using a panel of 218 world-wide Arabidopsis accessions, we characterized the natural variation in root suberization by quantifying 16 suberin monomers. We explored the associations between suberization traits and 126 climate variables. We conducted genome-wide association analysis and integrated previous genotype-environment association (GEA) to identify the genetic bases underlying suberization variation and their involvements in climate adaptation. Root suberin content displays extensive variation across Arabidopsis populations and significantly correlates with local moisture gradients and soil characteristics. Specifically, enhanced suberization is associated with drier environments, higher soil cation-exchange capacity, and lower soil pH; higher proportional levels of very-long-chain suberin is negatively correlated with moisture availability, lower soil gravel content, and higher soil silt fraction. We identified 94 putative causal loci and experimentally proved that GPAT6 is involved in C16 suberin biosynthesis. Highly significant associations between the putative genes and environmental variables were observed. Roots appear highly responsive to environmental heterogeneity via regulation of suberization, especially the suberin composition. The patterns of suberization-environment correlation and the suberin-related GEA fit the expectations of local adaptation for the polygenic suberization trait.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Estudio de Asociación del Genoma Completo , Raíces de Plantas/genética , SueloRESUMEN
Cuticular lipids consisting of cutin and wax coat aerial plant surfaces providing protection against biotic and abiotic stresses. Although much progress has been made on comprehending the regulation of plant cuticular lipid biosynthesis, their functional relevance in plant protection merits further investigation of potential regulators of their synthesis. HRD1 and DOA10 mediate two major Endoplasmic Reticulum-Associated Degradation (ERAD) pathways in yeast and also regulate common pathways during lipid metabolism. However, their roles in plant lipid metabolism are not well studied. CER9, an Arabidopsis homolog of DOA10, is known to play important roles in cuticular lipid biosynthesis. This prompted us to determine if HRD1 also plays a role in regulation cuticular lipid biosynthesis. Here we report that an Arabidopsis hrd1a hrd1b double mutant is impacted in the accumulation of both cutin and cuticular waxes including a large increase in total stem cutin with a concomitant decrease in stem wax content. We further investigated genetic relationship between HRD1A/1B- and CER9-mediated ERAD pathways with regard to cuticular lipid synthesis. Surprisingly, simultaneous mutation of HRD1 and CER9 revealed additive effects on stem wax synthesis, but not stem cutin synthesis. Collectively, our study advances our understanding of the ERAD regulatory roles in cuticular lipid synthesis identifying HRD1 as an important player in the regulated deposition of Arabidopsis stem cuticular lipids.