Asunto(s)
Anticuerpos Antibacterianos/sangre , Vacuna contra la Peste/inmunología , Peste/inmunología , Yersinia pestis/inmunología , Animales , Antígenos Bacterianos/inmunología , Epítopos/inmunología , Cobayas , Immunoblotting/métodos , Ratones , Peso Molecular , Peste/prevención & control , Especificidad de la EspecieRESUMEN
The presence of several active foci of infection of different etiology is an indication for complex (combined) immunization against these diseases. The scheme of complex (combined) immunization against plague, cholera and yellow fever has been experimentally substantiated and successfully tested on volunteers.
Asunto(s)
Vacunas contra el Cólera/inmunología , Vacuna contra la Peste/inmunología , Cuarentena , Vacunas Virales/inmunología , Virus de la Fiebre Amarilla/inmunología , Adolescente , Adulto , Animales , Cólera/inmunología , Cólera/prevención & control , Vacunas contra el Cólera/efectos adversos , Evaluación de Medicamentos , Evaluación Preclínica de Medicamentos , Cobayas , Humanos , Ratones , Ratones Endogámicos BALB C , Papio , Peste/inmunología , Peste/prevención & control , Vacuna contra la Peste/efectos adversos , Conejos , Ratas , Vacunas Combinadas/efectos adversos , Vacunas Combinadas/inmunología , Vacunas Virales/efectos adversos , Fiebre Amarilla/inmunología , Fiebre Amarilla/prevención & controlRESUMEN
The work is devoted to the effect of vaccine strain of the plague microbe on the expression of C3-receptors (C3R) in interaction with macrophages in vitro and in vivo. It is established that the activation of macrophages accompanied by the increase of C3R expression on the external surface of the membrane of both peritoneal and alveolar macrophages occurs in the process of formation of antiplague immunity. Maximum activity of C3R in the both populations of macrophages is observed on the 7th day after vaccination. Immunization of the plague microbe by the vaccine strain does not change the character of response of peritoneal and alveolar macrophages to their interaction with this microorganism in vitro: a decrease of C3R expression on the surface of the both populations of cells obtained from both intact and immunized animals is observed. Heterogeneity of the studied populations as to C3R expression both in the intact and immune organism is detected.
Asunto(s)
Complemento C3/inmunología , Regulación Bacteriana de la Expresión Génica/inmunología , Macrófagos Alveolares/inmunología , Macrófagos Peritoneales/inmunología , Vacuna contra la Peste/inmunología , Receptores de Complemento/inmunología , Yersinia pestis/inmunología , Animales , Cobayas , Inmunización , Activación de Macrófagos/inmunología , Vacuna contra la Peste/genética , Formación de Roseta , Propiedades de Superficie , Factores de Tiempo , Yersinia pestis/genéticaRESUMEN
The study of alveolar macrophages obtained from guinea pigs at different periods after the subcutaneous immunization with Y. pestis vaccine strain has revealed the heterogeneity of these macrophages in their bactericidal activity with respect to Y. pestis and in their resistance to the cytopathic action of this microorganism, as well as in the content of lysosomes and in the expression of receptors on the cell surface. The study has shown that subcutaneous immunization does not lead to the redistribution of subpopulations and to changes in their digestive activity.
Asunto(s)
Vacunas Bacterianas/inmunología , Macrófagos/inmunología , Alveolos Pulmonares/inmunología , Yersinia pestis/inmunología , Animales , Vacunas Bacterianas/administración & dosificación , Separación Celular , Complemento C3/inmunología , Cobayas , Inmunización/métodos , Inyecciones Subcutáneas , Lisosomas/inmunología , Activación de Macrófagos/inmunología , Fagocitosis/inmunología , Receptores de Complemento/inmunología , Receptores Fc/inmunología , Factores de TiempoRESUMEN
In 4 Francisella tularensis strains varying in virulence a receptor to Fc site of human IgG has been detected. This receptor consists of two active components with molecular weights of 67,000 and 40,000, competing for binding on Fc site of human IgG with Staphylococcus aureus protein A.
Asunto(s)
Francisella tularensis/inmunología , Inmunoglobulina G/inmunología , Receptores Fc/inmunología , Vacunas Bacterianas/inmunología , Unión Competitiva , Humanos , Immunoblotting , Técnicas para Inmunoenzimas , Peso Molecular , Proteína Estafilocócica A/inmunología , Staphylococcus aureus/inmunologíaRESUMEN
The expression of Fc gamma R on the surface of macrophages in the process of antiplague immunity formation is analyzed. The stud is performed on the alveolar and peritoneal macrophages obtained from intact and immunized guinea pigs in different periods after vaccination (the 1st, 7th, 14th and 21st day). It is established that during the formation of the antiplague immunity there occurs activation of macrophages which is accompanied by an increase of the Fc gamma R expression on the outer surface of the membrane both of peritoneal and alveolar macrophages and the pattern of response of these cells to the interaction with the vaccine strain of the plague microbe changes. The Fc gamma R expression heterogeneity of certain macrophage populations is revealed both in an intact and in immune organism as well as different pattern of the intact alveolar and peritoneal macrophage response during the interaction with the vaccine strain of the antiplague microbe. These differences are levelled in the process of the antiplague immunity formation.
Asunto(s)
Antígenos de Diferenciación/inmunología , Vacunas Bacterianas/inmunología , Activación de Macrófagos/inmunología , Macrófagos/inmunología , Receptores Fc/inmunología , Yersinia pestis/inmunología , Animales , Antígenos de Diferenciación/análisis , Cobayas , Inmunización , Macrófagos/análisis , Peste/inmunología , Peste/prevención & control , Receptores Fc/análisis , Receptores de IgG , Formación de Roseta , Propiedades de Superficie , Factores de Tiempo , Virulencia/inmunología , Yersinia pestis/patogenicidadAsunto(s)
Cólera/prevención & control , Medicina Militar , Peste/prevención & control , Fiebre Tifoidea/prevención & control , Vacunación , Vacunas contra el Cólera/administración & dosificación , Humanos , Masculino , Vacuna contra la Peste/administración & dosificación , Vacunas Tifoides-Paratifoides/administración & dosificación , U.R.S.S.RESUMEN
Experiments in guinea-pigs have indicated that combined immunization with the mixture of live plague and smallpox vaccines introduced subcutaneously by means of a jet injector are safe and faintly reactogenic. Highly intense immunity, as revealed by serological shifts and by the degree of protection of the vaccinated animals challenge with the virulent cultures of the corresponding infective agents, allows the recommendation that combined subcutaneous immunization against plague and smallpox by means of a jet injector be further studied on humans.