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The increase of sheep meat competitiveness in international markets can be attributed to the rise of the quantity and the improvement of the quality of the edible portion of sheep carcasses. Usually, carcass yield is established after the slaughter of the animals. Yet, when carcass yield is determined in vivo, it can be both a costly and subjective method. This study proposes models for predicting the physical characteristics of lamb carcass using bioimpedance analysis (BIA) in live animals. Thirty-one Texel × Ile de France crossbreed ram lambs were slaughtered at 20, 26, 32 or 38 kg of BW. Before the slaughter, values of resistance (Rs) and reactance (Xc) were collected using a single-frequency BIA equipment (Model RJL Quantum II Bioelectrical Body Composition Analyzer). Then, BIA main variables such as body bioelectrical volume (V), phase angle (PA), resistive density (RsD) and reactive density (XcD) were calculated. After slaughter, cold carcass weight (CCW), cold carcass yield (CCY), subcutaneous fat thickness (SFT), soft tissue weight (STW) and soft tissue yield (STY) were also measured. Multiple regression analyses were carried out using the physical characteristics as dependent variables and the bioimpedance values as independent variables. Predictive performance of the models was assessed using leave-one-out cross-validation. The prediction model of CCW was obtained using the V, PA and RsD (R2 = 0.97), STW through the V, RsD and XcD (R2 = 0.97), CCY by Rs, Z and XcD (R2 = 0.69), STY by V and XcD (R2 = 0.67), and SFT only for XcD (R2 = 0.84). The results indicated that BIA has the potential to predict carcass characteristics of lambs at different body masses.

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Objetivou-se avaliar os efeitos do zinco ou do propilenoglicol sobre o desempenho e nas características da carcaça e da carne de ovinos sob pastejo em pastagem nativa da Caatinga. Utilizaram-se 24 ovinos, mestiços Santa Inês, machos, não castrados, peso inicial de 19,3±2,52kg, com quatro meses de idade, oito repetições por tratamento, distribuídos em um delineamento inteiramente ao acaso. Os tratamentos foram: sem aditivo, controle (CT), adição de sulfato de zinco (ZnSO4.7H2O) (Zn) para fornecimento de 300mg de Zn dia-1 via sal mineral, e propilenoglicol (PG), 2,5mL kgPC0,75-1 animal-1 dia-1 misturado no concentrado. Os animais foram suplementados com concentrado constituído de milho (54,0%), farelo de soja (45,1%) e calcário (0,911%) em 0,7% do PC médio de cada tratamento. O ensaio foi conduzido durante quatro meses (março a junho/2014). Avaliaram-se o desempenho e, após o abate dos animais, as características de carcaça e os componentes não carcaça, os parâmetros físico-químicos e organolépticos na carne. O Zn e o propilenoglicol não afetaram o desempenho e os parâmetros da carcaça em termos absoluto e relativo, bem como os pesos dos cortes e seus rendimentos (P>0,05). Não houve efeito dos aditivos nas características organolépticas e nos aspectos físico-químicos da carne (P>0,05), exceto cinzas (P<0,05). O zinco e o propilenoglicol não melhoram o desempenho e as características da carcaça de animais terminados em pastagem nativa da Caatinga. O baixo suprimento energético devido à má qualidade do pasto entre os meses de abril a junho contribui para a ineficiência no aproveitamento dos constituintes proteicos, implicando menor consumo e consequente diminuição do desempenho.(AU)

The aim was to evaluate the effects of zinc sulfate or propylene glycol on performance and carcass and meat characteristics from sheep under grazing in native Caatinga (Brazilian savannah) pasture during the rainy season. Twenty-four intact Santa Ines crossbreed male lambs, with an initial weight of 19.3±2.52kg, and 4-mo old, were distributed in a completely randomized design with eight replications per treatment. The treatments were composed of additives, as follows: no additive - control (CT); addition of zinc sulfate (ZnSO4.7H2O) (Zn) to supply a total of 300mg Zn day−1 on mineral salt; and addition of propylene glycol (PG), 2.5mL kg LW0.75−1 animal−1 day−1 mixed directly in the concentrate. Afterwards, they were supplemented with concentrate at 0.7% of the average LW of each batch according to the treatments. The concentrate was based on corn (54.0%), soybean meal (45.1%), and limestone (0.911%). The trials were carried out during the rainy season (March to June 2014), and evaluated the performance, and before of slaughter, carcass characteristics and non-carcass components were evaluated and the physical-chemical aspects and the organoleptic attributes on the meat. The additives did not affect the performance, parameters of carcass, in terms absolute and relative, carcass morphometric measurements, weight of the commercial cuts and yields (P>0.05). The additives did not affect the organoleptic characteristics and physical-chemical aspects of the meat (P>0.05), except, ash (P<0.05). The zinc sulfate and propylene glycol did not improve on performance and in carcass characteristics in lambs finished on a native Caatinga (Brazilian savannah) pasture. The low supply energetic due to poor quality of pasture between the months of April to June, which contributed to inefficiency in the utilization of proteics constituents, implying in lower intake, and decrease in performance.(AU)

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Four trials were conducted to evaluate the effects of flint corn processing and the replacement of corn with citrus pulp (CiP) in diets for Nellore feedlot cattle. In a 103-d finishing trial, 216 Nellore bulls (350 ± 24 kg initial BW) were used in a randomized complete block design with a 2 × 4 factorial arrangement of treatments. Factors included 2 processing methods, either ground corn (GC) or steam-flaked corn (FC), with CiP replacing each corn type at 4 levels (0, 25, 50, and 75% of DM). All diets contained 12% sugarcane bagasse and 88% concentrate (DM basis). Treatments were also evaluated in metabolism trials, in which 10 ruminally cannulated Nellore steers (389 ± 37 kg) were assigned to 2 independent but simultaneous 5 × 5 Latin squares, each using 1 method of corn processing (GC and FC). Interactions ( < 0.05) between corn processing and CiP inclusion level were observed for final BW, DMI, ADG, G:F, and HCW. With FC-based diets, added CiP linearly decreased final BW ( = 0.04), whereas with GC-based diets, added CiP quadratically increased final BW ( = 0.002). With FC-based diets, the inclusion of CiP linearly increased DMI ( = 0.03) and linearly decreased ADG ( = 0.03) and G:F ( = 0.001). Increasing CiP in GC-based diets quadratically increased DMI ( = 0.001), ADG ( = 0.005), and HCW ( = 0.003). In FC-based diets, CiP inclusion had no effect on HCW ( = 0.21). Dressing percent, LM area, and 12th-rib fat were not affected by diet ( ≥ 0.05). For steers fed GC diets, CiP inclusion in the diet quadratically decreased the molar proportion of isovalerate ( = 0.001) but linearly increased ruminal butyrate ( = 0.006). No differences ( ≥ 0.16) were observed for total VFA concentrations, acetate:propionate ratio, and ruminal NH-N as CiP replaced GC. For steers fed FC diets, the molar proportion of acetate linearly increased ( = 0.002) whereas the proportion of propionate was linearly decreased ( < 0.001), resulting in a linear increase ( = 0.001) in the acetate:propionate ratio. Replacing corn with CiP linearly reduced NEm ( = 0.001) and NEg ( < 0.001) of FC-based diets but did not affect ( = 0.15) NE values of CG-based diets. Steam flaking flint corn improved cattle performance in this trial more than has been reported for dent corn in the published literature.

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Um experimento foi conduzido com o objetivo de avaliar as características da carcaça e dos componentes não carcaça de cordeiros nascidos de parto simples ou duplo. Dezesseis cordeiros mestiços Texel × Ile de France, machos não castrados, foram desmamados aos 56 dias de idade e abatidos com aproximadamente 28kg de peso corporal. Imediatamente após o abate, cada componente corporal foi pesado separadamente, sendo as carcaças avaliadas após 24 horas de refrigeração a 2°C. Os cordeiros oriundos de parto simples apresentaram superioridade no peso e nos rendimentos da carcaça, além de maior área de olho de lombo. Por outro lado, proporcionalmente ao peso de corpo vazio, o rúmen é maior em cordeiros nascidos de parto duplo, o que resulta incremento da proporção de conteúdo gastrintestinal ao abate. Os resultados deste estudo indicam que, quando abatidos a um mesmo peso, cordeiros oriundos de parto simples possuem carcaças de melhor qualidade, em comparação a cordeiros nascidos de parto gemelar...

An experiment was conducted to evaluate the carcass and non-carcass traits of single and twin lambs. Sixteen Texel × Ile de France crossbred, non-castrated male lambs were weaned at 56 days of age and slaughtered at 28kg of body weight. Immediately after the slaughter each body component was individually weighed, while carcasses were evaluated after a 24h period of refrigeration at 2°C. Single lambs presented higher values of carcass weight and carcass dressing percentage. Moreover, the Longissimus muscle area was also greater for single lambs. On the other hand, as a proportion of empty body weight, rumen was greater for twin lambs, increasing their gastrointestinal content at slaughter. The results of this study indicated that at a same slaughter weight single lambs have better quality carcasses in comparison with those from twin lambs...

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Poor-quality roughages are widely used as fiber sources in concentrate-based diets for ruminants. Because roughage quality is associated with the efficiency of energy use in forage-based diets, the objective of this study was to determine whether differing the roughage source in concentrate-based diets could change the energy requirements of growing lambs. Eighty-four 1/2 Dorper × 1/2 Santa Inês ram lambs (18.0 ± 3.3 kg BW) were individually penned and divided into 2 groups according to primary source of dietary roughage: low-quality roughage (LQR; sugarcane bagasse) or medium-quality roughage (MQR; coastcross hay). Diets were formulated to be isonitrogenous (2.6% N) and to meet 20% of physically effective NDF. After a 10-d ad libitum adaptation period, 7 lambs from each group were randomly selected and slaughtered (baseline). Twenty-one lambs in each diet group were fed ad libitum and slaughtered at 25, 35, or 45 kg BW. The remaining 28 lambs (14 from each diet group) were submitted to 1 of 2 levels of feed restriction: 70% or 50% of the ad libitum intake. Retentions of body fat, N, and energy were determined. Additionally, 6 ram lambs (44.3 ± 5.6 kg BW) were kept in metabolic cages and used in a 6 × 6 Latin square experiment designed to establish the ME content of the 2 diets at the 3 levels of DM intake. There was no effect of intake level on diet ME content, but it was greater in the diet with LQR than in the diet with MQR (3.18 vs. 2.94 Mcal/kg, respectively; P < 0.01). Lambs fed the diet with LQR had greater body fat (g/kg of empty BW) and energy concentrations (kcal/kg of empty BW) because of a larger visceral fat deposition (P < 0.05). Using a low-quality roughage as a primary source of forage in a concentrate-based diet for growing lambs did not change NEm and the efficiency of ME use for maintenance, which averaged 71.6 kcal/kg(0.75) of shrunk BW and 0.63, respectively. On the other hand, the greater nonfibrous carbohydrate content of the diet with LQR resulted in a 17% better efficiency of ME use for gain (P < 0.01), which was associated with a greater partial efficiency of energy retention as fat (P < 0.01). This increased nutritional efficiency, however, should be viewed with caution because it is related to visceral fat deposition, a nonedible tissue.

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Two experiments were conducted to evaluate the relationship among the level of feed intake, chewing pattern, and diet digestibility in sheep fed a moderate-concentrate diet. The first experiment was conducted using six male lambs at a replicated 3 × 3 Latin square design to evaluate diet digestibility and microbial N synthesis according to the level of intake: ad libitum, or restricted to either 70 or 55% of the ad libitum intake. In the second experiment, fifteen male lambs were housed in individual stalls, in a completely randomized design, and fed one of the three levels of dry matter intake (DMI). Chewing patterns were then evaluated for 24 h using a regular 5-min interval observation technique, two times during the experimental period. Decreasing level of feed intake resulted in increased apparent digestibility of dry matter, organic matter, crude protein, and neutral detergent fibre, as well as of the true digestibility of organic matter. Total time spent eating and ruminating decreased with feed restriction. However, lambs fed at restricted levels of intake presented a higher rate of eating (g DMI/min) than those fed ad libitum, and spent more time ruminating each gram of DM (min/g DMI). In conclusion, our results suggest that a more effective chewing during rumination activity can have an important role on feed digestion in animals submitted to feed restriction.

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AIMS: To determine what skills make a good consultant haematological microscopist; to explore how these skills develop during training and to determine whether these skills are maintained following training. METHODS: Twenty consultant haematologists underwent a semistructured interview to explore these issues. The interviews were transcribed and analysed for common themes using the N-vivo analytic package. This provides rich subjective qualitative data as opposed to hard objective quantitative data. RESULTS: Experience, methodicity and interest were the commonest skills mentioned. However, 25% of interviewees admitted they no longer followed a format when reporting. Interviewees agreed they had passed from a hypothetico-deductive to a scheme-inductive diagnostic reasoning model during acquisition of expertise. Only 20% had undertaken refresher training since becoming consultants, but the majority undertake some peer review of their work. CONCLUSIONS: These skills could form the basis of vocational and revalidation assessments in the practice of haematological microscopy. The elucidation of such skill development can help refine standards and remedial training through the process of "deliberate practice." Finally, the low uptake of refresher courses for established consultants needs serious consideration.

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Two trials were conducted to determine the energy requirements of feedlot Texel crossbred lambs. In a comparative slaughter trial, thirty 11/16 Texel x 5/16 Ile de France crossbred noncastrated male lambs, weaned at 42 d of age (16.2 +/- 2.1 kg of shrunk BW; SBW), were used. Five lambs were randomly chosen and slaughtered after 10 d of experimental management and diet adaptation (baseline group). Fifteen lambs then were fed for ad libitum intake and slaughtered at 25, 30, or 35 kg of SBW. The remaining 10 lambs were randomly assigned to 2 levels of DMI, either 70 or 55% of the ad libitum intake, and were slaughtered concomitantly with lambs of the 35 kg of SBW group. Total body N, fat, and energy contents were determined. In a digestibility trial, 6 Texel x Ile de France crossbred lambs (30.4 +/- 2.6 kg of SBW) were housed in metabolic cages and used in a replicated 3 x 3 Latin square experiment to evaluate the energetic value of the diet at different feed intake levels. Net and ME requirements for maintenance were 58.6 and 91 kcal/kg(0.75) of SBW, respectively. Consequently, partial efficiency of energy use for maintenance was 0.64. Body fat content varied from 72.7 to 125.9 g/kg of empty BW, respectively, for 13.1 and 28.2 kg of empty BW. Net energy requirements for growth of lambs at 15 and 35 kg of SBW at an ADG of 250 g were 424 and 553 kcal/d, respectively. Partial efficiency of energy use for growth was 0.47. Texel x Ile de France crossbred growing lambs used in this study showed decreased nutritional requirements than those reported by most nutritional systems.

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Foram utilizados oito cordeiros castrados (35,0±6,0kg peso vivo) em um delineamento duplo quadrado latino 4 ´ 4 para avaliar o efeito de quatro níveis de fibra em detergente neutro (FDN) na dieta (25, 31, 37 e 43 por cento da matéria seca (MS)) sobre o consumo e parâmetros digestivos. O consumo de nitrogênio (N) variou de forma quadrática; o de MS, matéria orgânica (MO) e energia diminuíram (P<0,05) e de FDN aumentou (P<0,01) linearmente com o aumento de fibra na dieta. A digestibilidade da FDN e do N, assim como o pH e as concentrações de peptídeos no fluído ruminal foram similares em todos os tratamentos. A digestibilidade aparente da MS, da MO e da energia, assim como as concentrações ruminais de açúcares diminuíram linearmente (P<0,01) e a síntese de proteína microbiana ruminal, as concentrações ruminais de amônia e de aminoácidos variaram quadraticamente (P<0,05) com o aumento do teor de FDN das dietas. A redução do consumo e da digestibilidade foram mais evidentes somente pela inclusão do nível mais alto de FDN (43 por cento) e, sob o aspecto nutricional, a inclusão em torno de 30 por cento de FDN representa o nível mais adequado para formulação de dietas a base de silagem de sorgo e concentrado oferecido a cordeiros confinados.

Eight castrated raw lambs (35.0± 6.0kg live weight) were used in a replicated 4 ´ 4 latin square design to evaluate the effect of four neutral detergent fiber (NDF) levels (25, 31, 37 and 43 percent of dry matter (DM)) on intake and digestion traits. Nitrogen (N) intake varied quadratically; intake of DM, organic matter (OM) and energy decreased (P<0.05) and that of NDF increased (P<0.01) as fibre level increased. NDF and N digestibility, as well as pH and ruminal concentrations of peptides were similar for all treatments. Apparent digestibility of DM, OM and energy, as well as ruminal concentrations of sugars decreased (P<0.01) and, ruminal microbial protein synthesis and ruminal concentrations of ammonia and amino acids varied in a quadratic way (P<0.05) with increased levels of NDF. The effect of NDF in decreasing intake and digestibillity was more evident only at the highest level (43 percent). The inclusion of about 30 percent NDF represents the most adequate level for sorghum silage and concentrate based diets fed to feedlot lambs.

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A 65-year-old woman, referred to the endocrine clinic for investigation of tiredness and arthritis, was diagnosed as having diabetes on the basis of a positive family history and a raised glycated haemoglobin (HbA1) measurement. Subsequently, she was shown not to have diabetes but to have a persistently raised haemoglobin F (HbF) level. Initial assessment of HbF level by electroendosmosis demonstrated a normal HbF level but high values were confirmed using HPLC. Thalassaemia was excluded following DNA analysis. This case illustrates the importance of a glucose tolerance test following WHO criteria for the diagnosis of diabetes and emphasises that HbA1 is not a diagnostic test for diabetes.

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Addition of GTPgammaS to saponin-permeabilised human neutrophils activated both the NADPH oxidase and phospholipase D (PLD). This PLD activation was hardly affected by staurosporine or Ro31-8220 (at concentrations which inhibited PMA stimulated PLD activity), indicating that it was largely independent of protein kinase C (PKC). This GTPgammaS stimulated PLD activity was enhanced by 1 mM ATP, but this ATP-enhanced activity was blocked by inhibitors of PKC. Addition of GTPgammaS resulted in very low levels of phosphorylation on tyrosine residues, but higher levels of phosphorylation on serine/threonine residues. Addition of pervanadate hydroperoxides stimulated phosphorylation on tyrosine residues and activated PLD which was blocked by addition of inhibitors of tyrosine kinases. Thus, GTPgammaS can stimulate PKC-dependent and -independent pathways of PLD activation. Whilst phosphorylation on tyrosine residues can result in activation of PLD, this is regulated independently of activation via G-proteins.

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We have recently shown that interleukin 1 beta (IL-1 beta) directly reduces the number of adipocytes in cultures of human bone marrow (BM) stromal cells. The aim of the present study was to establish the mechanisms involved in the IL-1 effect and thereby assess its importance in BM pathophysiology. Direct morphological observation showed that individual adipocytes lost their fat in the presence of IL-1 beta and regained it when the cytokine was withdrawn. These morphological observations were supported by metabolic studies using [14]acetate as a precursor of storage fat. These metabolic studies showed that IL-1 beta inhibited the incorporation of label into triglycerides. In addition, the cytokine enhanced the release of radioactivity from prelabelled adipocytes and reduced their triglyceride stores. It was therefore concluded that IL-1 beta can both inhibit lipogenesis and stimulate lipolysis in BM adipocytes. Forskolin (an adenylate cyclase activator) produced lipolytic effect similar to those of IL-1 beta, while indomethacin (an inhibitor of prostaglandin [PG] production) fully blocked the release of radioactivity induced by IL-1 beta and greatly increased triglyceride synthesis. However, IL-1 beta was still able to decrease triglyceride synthesis in the presence of indomethacin. These results indicate that a prostaglandin-dependent increase in cAMP is important to in the lipolytic effects of IL-1 beta but that the anti-lipogenic effects of the cytokine are, at least in part, independent of PG synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)

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Bone marrow (BM) stromal cells express CD10 (cALLA), a surface antigen now known to be a neutral endopeptidase (NEP-24.11). The function of CD10 in BM stroma is unknown, although purified NEP-24.11 is known to degrade different substrates including interleukin 1 beta (IL-1 beta). We have therefore employed a CD10-positive BM stromal cell line (L2AK) which proliferates in response to IL-1 beta to test the hypothesis that degradation of this cytokine is one of the functions of stromal CD10. We first showed that [3H]thymidine incorporation by L2AK cells is enhanced by IL-1 beta in a clear dose-dependent manner. Addition of the CD10 inhibitor, phosphoramidon, together with IL-1 beta resulted in a left shift in the dose-response curve which corresponded to a 10-fold potentiation of the IL-1 beta effect. These results indicate that CD10 on bone marrow stromal cells can degrade IL-1 beta and therefore provide a local control of the effects of this, and possibly other, growth factor(s).

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