RESUMEN
A short survey of essential thrombocythaemia is made and some diagnostic and therapeutic aspects of the disease and personal clinical experience are discussed.
Asunto(s)
Trombocitemia Esencial/diagnóstico , Alquilantes/uso terapéutico , Antimetabolitos/uso terapéutico , Aspirina/uso terapéutico , Médula Ósea/efectos de los fármacos , Dipiridamol/uso terapéutico , Quimioterapia Combinada , Humanos , Radioisótopos de Fósforo/uso terapéutico , Recuento de Plaquetas/efectos de los fármacos , Inducción de Remisión , Trombocitemia Esencial/sangre , Trombocitemia Esencial/tratamiento farmacológicoRESUMEN
At the cell surface, passive transport is controlled by membrane proteins forming hydrophilic pores (or channels) which span the hydrophobic core of the lipid bilayer. Gating mechanisms determine the occurrence of very small and fast step-like current jumps. We developed an intelligent system to acquire and to analyze signals due to the opening and closing of ionic single channels. A specific algorithm allows us to recognize channel current transitions and to discard false events due to noise peaks or to unwelcome fluctuations of the signal. Statistics of the single channel amplitude and mean life-time can be performed. We report the results obtained from analyzing the characteristics of the gramicidin A single channel in phosphatidylserine model membranes. Mean current values of 2.08 +/- 0.01 pA and life-times of 101 +/- 3 ms. were measured at 100mV applied potentials in KC1 100mM solution.