RESUMEN
The purpose of this investigation was to compare concentric movement velocity (CMV) measured with the PUSH Band (v2.0) and a Vicon motion capture system (MC) during the back squat (SQ) and the bench press (BP) resistance exercises (RE). Twelve resistance-trained males (26.0 ± 5.5 years; 175.6 ± 4.9 cm; 96.3 ± 15.8 kg) completed ten repetitions at 50% of one-repetition maximum (1RM), and six repetitions at 75% 1RM for both BP and SQ. Four PUSH devices were utilized and attached to the subject's right forearm, the center barbell, left and right sides of the barbell. MC markers were placed on top of each PUSH device. An overall analysis using a series of least-squares means contrasts suggested CMV did not differ (p > 0.05) between measurement technologies when position, RE, intensity and repetitions were combined. PUSH exhibited the highest Intraclass Correlation Coefficients (ICC = 0.835−0.961) and Pearson Product-Moment Correlation Coefficients (r = 0.742−0.949) at the arm and center barbell locations when compared with MC. The measurement of CMV between MC and PUSH compares favorably during moderate (i.e., 50%) and high (75%) intensity SQ and BP RE. These data indicate individuals can use the PUSH band v2.0 to accurately monitor CMV within a RE set for SQ and BP RE.
RESUMEN
The use of porous 3D scaffolds for the repair of bone nonunion and osteoporotic bone is currently an area of great interest. Using a combination of thermally-induced phase separation (TIPS) and 3D-plotting (3DP), we have generated hierarchical 3DP/TIPS scaffolds made of poly(lactic-co-glycolic acid) (PLGA) and nanohydroxyapatite (nHA). A full factorial design of experiments was conducted, in which the PLGA and nHA compositions were varied between 6-12% w/v and 10-40% w/w, respectively, totaling 16 scaffold formulations with an overall porosity ranging between 87%-93%. These formulations included an optimal scaffold design identified in our previous study. The internal structures of the scaffolds were examined using scanning electron microscopy and microcomputed tomography. Our optimal scaffold was seeded with MC3T3-E1 murine preosteoblastic cells and subjected to cell culture inside a tissue culture dish and a perfusion bioreactor. The results were compared to those of a commercial CellCeram™ scaffold with a composition of 40% ß-tricalcium phosphate and 60% hydroxyapatite (ß-TCP/HA). Media flow within the macrochannels of 3DP/TIPS scaffolds was modeled in COMSOL software in order to fine tune the wall shear stress. CyQUANT DNA assay was performed to assess cell proliferation. The normalized number of cells for the optimal scaffold was more than twofold that of CellCeram™ scaffold after two weeks of culture inside the bioreactor. Despite the substantial variability in the results, the observed improvement in cell proliferation upon culture inside the perfusion bioreactor (vs. static culture) demonstrated the role of macrochannels in making the 3DP/TIPS scaffolds a promising candidate for scaffold-based tissue engineering.
RESUMEN
This paper reports on the hybrid process we have used for producing hierarchical scaffolds made of poly(lactic-co-glycolic) acid (PLGA) and nanohydroxyapatite (nHA), analyzes their internal structures via scanning electron microscopy, and presents the results of our in vitro proliferation of MC3T3-E1 cells and alkaline phosphatase activity (ALP) for 0 and 21 days. These scaffolds were produced by combining additive manufacturing (AM) and thermally induced phase separation (TIPS) techniques. Slow cooling at a rate of 1.5 °C/min during the TIPS process was used to enable a uniform temperature throughout the scaffolds, and therefore, a relatively uniform pore size range. We produced ten different scaffold compositions and topologies in this study. These scaffolds had macrochannels with diameters of â¼300 µm, â¼380 µm, and â¼460 µm, generated by the extraction of embedded porous 3D-plotted polyethylene glycol (PEG) matrices. The other experimental factors included different TIPS temperatures (-20 °C, -10 °C, and 0 °C), as well as varying PLGA concentrations (8%, 10%, and 12% w/v) and nHA content (0%, 10%, and 20% w/w). Our results indicated that almost all these macro/microporous scaffolds supported cell growth over the period of 21 days. Nevertheless, significant differences were observed among some scaffolds in terms of their support of cell proliferation and differentiation. This paper presents the results of our in vitro cell culture for 0 and 21 days. Our optimal scaffold with a porosity of â¼90%, a modulus of â¼5.2 MPa, and a nHA content of 20% showed a cell adhesion of â¼29% on day 0 and maintained cell proliferation and ALP activity over the 21-day in vitro culture. Hence, the use of additive manufacturing and designed experiments to optimize the scaffold fabrication parameters resulted in superior mechanical properties that most other studies using TIPS.