RESUMEN
One of the purposes of this study was to examine whether b-wave measurements can be used in the evaluation of scotopic spectral sensitivity in Prussian carp measurements when the eyes were surgically deprived of cornea, lens, and most of the vitreous. Another goal was testing the new fitting procedure for A2-based photopigments. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral sensitivity curves, no significant differences in λ(max) were found between rod photopigments and b-wave-based spectral sensitivity.
Asunto(s)
Carpas/fisiología , Adaptación a la Oscuridad/fisiología , Retina/fisiología , Animales , Electrorretinografía/veterinariaRESUMEN
In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve "Uvac", as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve "Uvac" showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased.
Asunto(s)
Cyprinidae , Daño del ADN , Contaminantes Químicos del Agua/toxicidad , Animales , Ensayo Cometa , Monitoreo del Ambiente , Eritrocitos/efectos de los fármacos , Eritrocitos/metabolismo , Branquias/citología , Branquias/metabolismo , Hígado/citología , Hígado/metabolismo , Ríos , SerbiaRESUMEN
The genotoxic potential of waters in six rivers and reservoirs from Serbia was monitored in different tissues of chub (Squalius cephalus L. 1758) with the alkaline comet assay. The comet assay, or single-cell gel electrophoresis, has a wide application as a simple and sensitive method for evaluating DNA damage in fish exposed to various xenobiotics in the aquatic environment. Three types of cells, erythrocytes, gill cells, and liver cells, were used for assessing DNA damage. Images of randomly selected cells were analyzed with a Leica fluorescence microscope and image analysis by software (Comet Assay IV Image analysis system, PI, UK). Three parameters (tail length-l, tail intensity-i, and Olive tail moment-m) were analyzed on 1,700 nuclei per cell type. The procedure for sum of ranking differences (SRD) was implemented to compare different types of cells and different parameters for estimation of DNA damage. Regarding our nine different estimations of genotoxicity: tail length, intensity, and moment in erythrocytes (rel, rei, rem), liver cells (rll, rli, rlm), and gill cells (rgl, rgi, rgm), the SRD procedure has shown that the Olive tail moment and tail intensity are (almost) equally good parameters; the SRD value was lower for the tail moment and tail intensity than for tail length in the case of all types of cells. The least reliable parameter was rel; close to the borderline case were rei, rll, and rgl (~5 % probability of random ranking).
Asunto(s)
Ensayo Cometa/métodos , Cyprinidae/genética , Daño del ADN/genética , ADN/genética , Interpretación Estadística de Datos , Pruebas de Mutagenicidad/métodos , Xenobióticos/envenenamiento , Animales , ADN/efectos de los fármacos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
Comparative electroretinographic studies of the d-wave evoked with long duration photo stimuli in dark- and light-adapted fish species (three marine and three freshwater) were performed. At the end of prolonged photo-stimulation in scotopic conditions a negative d-wave appears in electroretinograms of dogfish shark, eel and goldfish diminishing and eventually changing with intensity of photo-stimulation, while in rudd it only increases. Dark-adapted electroretinograms of two percids (perch and painted comber) exhibit a positive d-wave that approaches the b-wave amplitude under bright photopic conditions. Judging from the d-wave, only the rod pathway is active in dark-adapted dogfish shark, eel, and goldfish. Under the light adaptation, cone pathways are active in eel and goldfish, whereas the positive response to the end of light stimuli in dogfish shark could be explained by independent ON and OFF pathways from outer to inner retina via bipolar cells. In the case of two percids, dark adaptation has no influence on cone pathways. The d-wave of rudd behaves like cone-driven d-waves but in opposite sign. The data thus show that the d-wave form, amplitude and sign depend on interconnection of ON and OFF pathways as determined by the state of adaptation and/or type of photoreceptor.
Asunto(s)
Adaptación Ocular/fisiología , Peces/fisiología , Retina/fisiología , Retina/efectos de la radiación , Adaptación Ocular/efectos de la radiación , Animales , Adaptación a la Oscuridad/efectos de la radiación , Electrorretinografía , Agua Dulce , Océanos y Mares , Retina/citología , Células Fotorreceptoras Retinianas Bastones/metabolismo , Células Fotorreceptoras Retinianas Bastones/efectos de la radiación , Factores de TiempoRESUMEN
In view of the use of synthetic propentofylline (PPF) as a protective agent in brain ischemia, its possible side effects on vision capacities have been explored by electroretinography in comparative experiments with theophylline. We used eyecup preparations of small-spotted dogfish sharks and of European eels, particularly suitable for long-lasting experiments. The drug exerted profound but reversible modifications of ERG records: (1) a dose-dependent increase of the amplitude and duration of the chemically isolated late receptor potential (LRP), (2) a partial unmasking of LRP, (3) a strong potentiation of the LRP-unmasking effect of low temperature, (4) a potentiation of light adaptation effects, and (5) a strong potentiation of the post-illumination hyperexcitability. The effects were explicable as due to a strong phosphodiesterase (PDE) inhibiting, cyclic guanosine monophosphate (cGMP) promoting, action of the drug. The effects were considerably stronger, or even of opposite sign, in comparison to those of the chemically related theophylline. PPF did not seriously affect the ERG c-wave originating in the pigment epithelium. The results suggested that the effects of PPF on vision may not seriously hamper the therapeutic use of the drug. They indicated, on the other hand, that PPF was a retinoactive drug of potential usefulness in the exploration of the complex biochemical events underlying visual transduction.
Asunto(s)
Fármacos Neuroprotectores/efectos adversos , Retina/efectos de los fármacos , Xantinas/efectos adversos , Adaptación Ocular/fisiología , Anguilla , Animales , Temperatura Corporal/fisiología , Cazón , Relación Dosis-Respuesta a Droga , Electrorretinografía , Técnicas In Vitro , Estimulación Luminosa , Células Fotorreceptoras/efectos de los fármacos , Células Fotorreceptoras/fisiología , Retina/citología , Retina/fisiología , Teofilina/efectos adversosRESUMEN
Although differences in visual pigments between developmental stages of the European eel are well known, the expected differences in spectral sensitivity have not been demonstrated at the electrophysiological level. In fact, one past electroretinographic study led to the conclusion that in eels there is no change in scotopic sensitivity, with increasing sexual maturity. In the present experiments, electroretinograms (ERGs) were recorded from in situ eyecups of immobilized eels Anguilla anguilla (L.) caught in coastal running waters. It was shown that the ERG b-wave is as good an indicator of spectral sensitivity as the unmasked late receptor potential (LRP) which directly reflects the responsiveness of photoreceptors. Complete spectral-sensitivity curves, based on b-wave thresholds and on thresholds of LRP subsequently isolated by means of sodium iodate, have been obtained in the same eel. Using fitted amplitude-log intensity functions for threshold calculation, and two models for computer-assisted fitting of spectral-sensitivity curves, significant differences in lambda max were found between yellow and silver developmental stages of the eel, identified by ocular index measurements.