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8 expert fencers were studied with a 3-dimensional motion analysis system. Each subject performed 10 flèche attacks toward a standardized target. Surface electromyography signals (EMG) were recorded of the deltoid pars clavicularis, infraspinatus and triceps brachii caput laterale muscles of the weapon arm. The recorded EMGs were averaged using EMG wavelet-transformation software. 4 phases were defined based on the arm kinematics and used to classify fencers into 2 groups. A first group of 4 fencers showed an early maximal elbow extension (Early MEE) whereas the second group presented a late maximal elbow extension (Late MEE). 2 EMG-classifications were based on this kinematical classification, one in the time-domain and the other in the frequency-domain by using the spherical classification. The time-domain EMG-classification showed a significantly ( P=0.03) higher normalized deltoid intensity for the Early MEE group (91 ± 18%) than the Late MEE group (36 ± 13%) in the attack phase. The spherical classification revealed that the activity of all the muscles was significantly classified (recognition rate 75%, P=0.04) between the 2 groups. This study of EMG and kinematics of the weapon upper limb in fencing proposes several classifications, which implies a relationship between kinematic strategies, muscular activations and fencing success.

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Detection of mycobacterial infection can be achieved by different means; however, culture-based methods remain the gold standard. In this paper, we present a new culture-based method using real-time microcalorimetric detection of growth of Mycobacterium species including Mycobacterium tuberculosis. Microcalorimetric detection of heat production by 6 different growing species of Mycobacterium was achieved between 20 and 310h depending on their type (fast vs. slow-growing mycobacteria) and initial concentration. This study demonstrates that microcalorimetric detection of mycobacterial growth is a potential advantageous alternative to methods using fluorescent or radiolabeled growth indicators.

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The purpose of this orthopaedic-biomechanical study was to evaluate the muscle function in total ankle replacement (TAR) patients 1 year after surgery. Ten patients underwent a combined clinical and muscle biomechanical assessment prior to implantation and at the 1-year follow-up. Pain score, American Orthopaedic Foot and Ankle Society (AOFAS) ankle score, ankle range of motion (ROM), and calf circumference difference between the affected leg and contralateral healthy leg were assessed. Biomechanically, isometric maximal voluntary torque for ankle dorsiflexion and plantar flexion was measured simultaneously with surface electromyography of four lower leg muscles. At follow-up, a significant improvement of the pain score (from 6.7 to 0.8 points), AOFAS ankle score (from 35.6 to 92.3 points), and ROM could be shown. Not significantly, the mean calf circumference difference between legs decreased from 2.2 to 1.4 cm. However, a significant increase was seen in the mean dorsiflexion (from 17.0 to 25.8 Nm) and plantar flexion torque (15.7 to 24.6 Nm) of the TAR-treated ankle. The mean EMG frequency content of the affected lower leg at TAR follow-up was lower than in the muscles of the contralateral healthy side. In contrast, the mean EMG intensity at TAR follow-up in side-comparison was statistically the same for all muscles. Ankle OA patients have better muscle function with TAR than under the arthritic condition, but they do not reach the normal level of the contralateral healthy leg 1 year after surgery.

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Stiffness is a fundamental indicator of the functional state of articular cartilage. Reported test modes include compressive incremental strain to determine the equilibrium modulus, and sinusoidal strain to determine the dynamic modulus and stress/strain loss angle. Here, initial development is described for a method recognizing that gait is pulsatile. Agarose gels have been used by others for validation or comparison of mechanical test methods and models for cartilage and proteoglycan aggregate. Accordingly, gels ranging from 0.5 to 20% agarose were prepared. Pulsatile stiffness in both indentation and unconfined compression were closely reproducible. Stiffness as a function of agarose concentration rose exponentially, as found using other methods. Indentation stiffness was higher than for unconfined compression and ranged from approximately 2.0 kPa for 0.5% gel to approximately 3,800 kPa for 20% gel. Pulsatile dynamic stiffness appears to be a useful method, although further development is needed. Agarose gel stiffness values obtained by other methods were reviewed for comparison. Unfortunately, reported values for a given agarose concentration ranged widely (e.g. fourfold) even when test methods were similar. Causes appear to include differences in molecular weight and gel preparation time-temperature regimens. Also, agarose is hygroscopic, leading to unintended variations in gel composition. Agarose gels are problematic materials for validation or comparison of cartilage mechanical test methods and models.

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The comparison of electromyograms represents a challenge for data analysis. The aim of the project was to present a method that uses a minimal computational effort to resolve small but significant changes in the muscular activity that occur while walking with and without a knee brace. The wavelet transformed electromyograms were represented as intensity patterns that resolve the power of the signal in time and frequency. The intensity pattern of each electromyogram defines single points in a pattern space. The distance between these points in pattern space were used to detect and show the separation between the groups of electromyograms that were recorded while walking with and without a knee brace. The method proposes a distance versus angle representation to visually discriminate the intensity patterns. Once it has been shown that the differences are statistically significant, one can visualize the result in a difference intensity pattern that indicates at what time and at what frequency the electromyograms vary between the two conditions tested. It is to be expected that interventions that are more intrusive than a knee brace will reveal even more distinct differences.

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