RESUMEN
INTRODUCTION: the knowledge and practices on Lassa fever (LF) infection prevention and control (IPC) remains poor among health workers in Nigeria despite LF endemicity. This study aimed to evaluate the knowledge, attitude, and practices of healthcare workers at the Federal Medical Centre, Owo towards LF. METHODS: this was a cross-sectional study among 451 healthcare workers who were enrolled using a simple random sampling technique. Data were collected using a semi-structured interviewer-administered questionnaire and analyzed with SPSS version 23. Adequate knowledge, positive attitude, and good practice of LF infection, prevention, and control were determined by the proportion of respondents who scored >80% in each category. Descriptive statistics were done. Associations were explored using Chi-square tests. RESULTS: the mean age of respondents was 37.95±8.43 years, and 169 (37.5%) were doctors. The mean overall knowledge score was 18.33±2.14, and 236 (52.3%) had appropriate knowledge, 109 (24.2%) had a positive attitude, while 351 (77.8%) demonstrated adequate preventive practices towards LFIPC. Laboratory scientists had five times the odds of appropriate knowledge of LF IPC (OR=4.886; 95%CI: 1.580-15.107). Pharmacists had ten times odds of positive attitude towards LF IPC (OR=10.093; 95%CI= 1.055-95.516). Pharmacists had nine times odds of good LF IPC practices (OR=8.755; 95%CI=1.028-74.531). CONCLUSION: disparities in knowledge, attitude, and practices of LF IPC exist among healthcare workers. To strengthen IPC, intervention strategies like training to address such gaps are needed.