RESUMEN

Congenital Toxoplasma gondii infection can result in intracranial calcification, hydrocephalus and retinochoroiditis. Acquired infection is commonly associated with ocular disease. Pathology is characterized by strong proinflammatory responses. Ligation of ATP by purinergic receptor P2X(7), encoded by P2RX7, stimulates proinflammatory cytokines and can lead directly to killing of intracellular pathogens. To determine whether P2X(7) has a role in susceptibility to congenital toxoplasmosis, we examined polymorphisms at P2RX7 in 149 child/parent trios from North America. We found association (FBAT Z-scores +/-2.429; P=0.015) between the derived C(+)G(-) allele (f=0.68; OR=2.06; 95% CI: 1.14-3.75) at single-nucleotide polymorphism (SNP) rs1718119 (1068T>C; Thr-348-Ala), and a second synonymous variant rs1621388 in linkage disequilibrium with it, and clinical signs of disease per se. Analysis of clinical subgroups showed no association with hydrocephalus, with effect sizes for associations with retinal disease and brain calcifications enhanced (OR=3.0-4.25; 0.004

Asunto(s)

Coriorretinitis/genética , Predisposición Genética a la Enfermedad/genética , Receptores Purinérgicos P2/genética , Toxoplasmosis Congénita/genética , Adulto , Brasil , Preescolar , Coriorretinitis/etiología , Femenino , Estudio de Asociación del Genoma Completo , Haplotipos/genética , Humanos , Patrón de Herencia/genética , Desequilibrio de Ligamiento , Modelos Logísticos , Masculino , América del Norte , Polimorfismo de Nucleótido Simple/genética , Receptores Purinérgicos P2X7 , Toxoplasmosis Congénita/complicaciones