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1.
Parasitol Res ; 117(7): 2351-2357, 2018 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-29777336

RESUMEN

The canid parasites Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi) parasitize the lower and the upper respiratory tract, respectively. Reports and descriptions of these nematodes are scarce in Argentina, possibly due to misdiagnosis of morphologically similar trichuroids eggs, and the lack of knowledge about the species of Eucoleus in this geographical area. Scanning electron microscopy is a useful tool for identification of E. boehmi eggs based on the characteristics of the shell structure which differentiate between species. Molecular analysis complements morphological identification. Until now, there are no studies based on the analysis of E. boehmi eggs in Argentina. The aim of the present work was to study by morphological, morphometric, and molecular analysis, eggs attributable to E. boehmi isolated from dogs naturally infected in Mar del Plata city, Argentina. Eggs isolated from two dog fecal samples were analyzed by light and scanning electron microscopy. A fragment of mitochondrial DNA (mtDNA) of the cytochrome c oxidase subunit I gene (cox1) from eggs was sequenced, and phylogenetic analysis was performed in this study. According to morphological results based on the wall surface ultrastructure, the eggs studied were assigned to E. boehmi. Molecular analysis supported the morphological identification. The divergence of 9-12% with the European isolated could suggest a new geographical genetic variation of E. boehmi, but also question the possible existence of cryptic species. This is the first characterization of E. boehmi eggs in dogs from Argentina.


Asunto(s)
Capillaria/aislamiento & purificación , Enfermedades de los Perros/parasitología , Perros/parasitología , Infecciones por Enoplida/veterinaria , Óvulo/citología , Animales , Argentina , Ciclooxigenasa 1 , ADN Mitocondrial/genética , Complejo IV de Transporte de Electrones/genética , Infecciones por Enoplida/parasitología , Heces/parasitología , Nariz/parasitología , Filogenia , Sistema Respiratorio/parasitología
2.
Parasitol Res ; 114(7): 2459-64, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25859925

RESUMEN

Knowledge of parasitic infections to which fauna was exposed in the past provides information on the geographical origin of some parasites, on the possible dispersal routes and for archaeological fauna on the potential zoonotic risk that human and animal populations could be exposed. The aim of the present study was to examine the gastrointestinal parasite present in camelid coprolites collected from the archaeological site Cerro Casa de Piedra, cave 7 (CCP7), Patagonia, Argentina. Coprolites were collected from different stratified sequences dating from the Pleistocene-Holocene transition to the late Holocene. Paleoparasitological examination revealed the presence of eggs of Trichostrongylidae attributed to Lamanema chavezi or Nematodirus lamae, eggs of three unidentified capillariids, Strongylus-type eggs and oocysts of Eimeria macusaniensis. These parasites affected camelids living in the studied area since the Pleistocene-Holocene transition, about 10,000 years ago. Gastrointestinal parasite fauna of patagonian camelids did not vary significatively from Pleistocene-Holocene transition to late Holocene, although environmental conditions fluctuated greatly throughout this period, as indicative of the strength and the stability of these associations over time. In this study, the zoonotic and biogeography importance of parasites of camelids are also discussed.


Asunto(s)
Biodiversidad , Heces/parasitología , Fósiles/parasitología , Parasitosis Intestinales/parasitología , Parásitos/aislamiento & purificación , Animales , Argentina , Camélidos del Nuevo Mundo/parasitología , Fósiles/historia , Historia Antigua , Humanos , Parasitosis Intestinales/historia , Paleopatología , Parásitos/clasificación , Parásitos/genética
3.
Parasitol Int ; 62(5): 431-4, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23602736

RESUMEN

Results of paleoparasitological examination of rodent coprolites collected from archaeological and paleontological sites from Patagonia, Argentina, are present. Each coprolite was processed, rehydrated, homogenized, spontaneously sedimented and examined using light microscope. Coprolites and eggs were described, measured and photographed, and were compared with current faeces of Lagidium viscacia. Eggs with morphological features, attributed to an anoplocephalid cestode were found in samples collected from Cueva Huenul 1 (36°56'45″S, 69°47'32″W, Neuquén Province, Holocene) and Los Altares Profile (43º53'35″S, 68º23'21″W, Chubut Province, Late Holocene). These are the first findings of this anoplocephalid from faecal material from patagonic rodents.


Asunto(s)
Cestodos/clasificación , Heces/parasitología , Fósiles , Óvulo/clasificación , Roedores , Animales , Argentina , Cestodos/aislamiento & purificación , Paleopatología
4.
Acta Trop ; 125(3): 282-6, 2013 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-23200641

RESUMEN

Paleoparasitological studies of the Brazilian colonial period are scarce. A paleoparasitological analysis was performed on human remains from the archeological site Praça XV Cemetery in Rio de Janeiro, dating from the early 18th to 19th Centuries. The samples were obtained from the Institute of the Brazilian Archaeology collection, and showed evidence of washing and brushing. Sediments were extracted from sacral foramina by scraping. Sediments from skulls were used as negative paleoparasitological controls. Spontaneous sedimentation method was performed prior to microscopic analysis. The results revealed that 8 of 10 individuals were infected with intestinal helminths and/or protozoa. Eggs of the nematodes Trichuris sp. and Ascaris sp. as well as a single taeniid egg were found. Protozoa cysts suggestive of Entamoeba sp. were also observed. Trichuris sp. was the most frequent and abundant parasite, found in 70% of individuals (26 eggs). The study showed the importance of analysis of sediment from human remains preserved in museum or scientific collections, even those subjected to a curating procedure. The levels of infection revealed here should be considered underestimations. This is the first paleoparasitological study from Rio de Janeiro city for the Brazilian colonial period and the first report of human Taenia sp. in the New World.


Asunto(s)
Fósiles , Parásitos/clasificación , Parásitos/aislamiento & purificación , Enfermedades Parasitarias/parasitología , Adolescente , Adulto , Animales , Brasil/epidemiología , Niño , Preescolar , Femenino , Historia del Siglo XVIII , Humanos , Masculino , Enfermedades Parasitarias/epidemiología , Enfermedades Parasitarias/historia
5.
Mem Inst Oswaldo Cruz ; 107(5): 604-8, 2012 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-22850950

RESUMEN

The aim of the present study was to examine the parasite fauna present in rodent coprolites collected from Cueva Huenul 1 (CH1), northern Neuquén (Patagonia, Argentina), an archaeological site that provides stratified sequences of archaeological and palaeontological remains dating from the Late Pleistocene/Early Holocene Transition to the Late Holocene period. Twenty rodent coprolites collected from different sedimentary units from the site, with ages ranging from 13.844 ± 75-1.416 ± 37 years BP, were examined for parasites. Each coprolite was processed as a whole: rehydrated, homogenised, spontaneously sedimented and examined using light microscopy. The coprolites and the eggs of any parasites present were described, measured and photographed. In all, 158 parasite eggs were found in 10 coprolites. The faeces were positive for Viscachataenia quadrata Denegri, Dopchiz, Elissondo & Beveridge and Monoecocestus sp. Beddard (Cestoda: Anoplocephalidae) and for Heteroxynema (Cavioxyura) viscaciae Sutton & Hugot (Nematoda: Oxyuridae). The coprolites examined were tentatively attributed to Lagidium viscacia Molina (Mammalia, Rodentia, Caviomorpha, Chinchillidae). The life cycles of these parasites are discussed.


Asunto(s)
Heces/parasitología , Paleopatología , Roedores/parasitología , Animales , Argentina , Fósiles
6.
Mem. Inst. Oswaldo Cruz ; 107(5): 604-608, Aug. 2012. ilus
Artículo en Inglés | LILACS | ID: lil-643745

RESUMEN

The aim of the present study was to examine the parasite fauna present in rodent coprolites collected from Cueva Huenul 1 (CH1), northern Neuquén (Patagonia, Argentina), an archaeological site that provides stratified sequences of archaeological and palaeontological remains dating from the Late Pleistocene/Early Holocene Transition to the Late Holocene period. Twenty rodent coprolites collected from different sedimentary units from the site, with ages ranging from 13.844 ± 75-1.416 ± 37 years BP, were examined for parasites. Each coprolite was processed as a whole: rehydrated, homogenised, spontaneously sedimented and examined using light microscopy. The coprolites and the eggs of any parasites present were described, measured and photographed. In all, 158 parasite eggs were found in 10 coprolites. The faeces were positive for Viscachataenia quadrata Denegri, Dopchiz, Elissondo & Beveridge and Monoecocestus sp. Beddard (Cestoda: Anoplocephalidae) and for Heteroxynema (Cavioxyura) viscaciae Sutton & Hugot (Nematoda: Oxyuridae). The coprolites examined were tentatively attributed to Lagidium viscacia Molina (Mammalia, Rodentia, Caviomorpha, Chinchillidae). The life cycles of these parasites are discussed.


Asunto(s)
Animales , Heces/parasitología , Paleopatología , Roedores/parasitología , Argentina , Fósiles
8.
Mem Inst Oswaldo Cruz ; 105(1): 33-40, 2010 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-20209326

RESUMEN

The aim of this study was to examine the parasite remains present in rodent coprolites collected from the archaeological site Alero Destacamento Guardaparque (ADG) located in the Perito Moreno National Park (Santa Cruz Province, 47 degrees 57'S 72 degrees 05'W). Forty-eight coprolites were obtained from the layers 7, 6 and 5 of ADG, dated at 6,700 +/- 70, 4,900 +/- 70 and 3,440 +/- 70 years BP, respectively. The faecal samples were processed and examined using paleoparasitological procedures. A total of 582 eggs of parasites were found in 47 coprolites. Samples were positive for eggs of Trichuris sp. (Nematoda: Trichuridae), Calodium sp., Eucoleus sp., Echinocoleus sp. and an unidentified capillariid (Nematoda: Capillariidae) and for eggs of Monoecocestus (Cestoda: Anoplocephalidae). Quantitative differences among layer for both coprolites and parasites were recorded. In this study, the specific filiations of parasites, their zoonotic importance, the rodent identity, on the basis of previous zooarchaeological knowledge, and the environmental conditions during the Holocene in the area are discussed.


Asunto(s)
Cestodos/aislamiento & purificación , Heces/parasitología , Fósiles , Helmintiasis Animal/historia , Nematodos/aislamiento & purificación , Roedores/parasitología , Animales , Argentina , Helmintiasis Animal/parasitología , Historia Antigua , Paleopatología , Recuento de Huevos de Parásitos
9.
Mem. Inst. Oswaldo Cruz ; 105(1): 33-40, Feb. 2010. ilus, tab
Artículo en Inglés | LILACS | ID: lil-539293

RESUMEN

The aim of this study was to examine the parasite remains present in rodent coprolites collected from the archaeological site Alero Destacamento Guardaparque (ADG) located in the Perito Moreno National Park (Santa Cruz Province, 47º57'S 72º05'W). Forty-eight coprolites were obtained from the layers 7, 6 and 5 of ADG, dated at 6,700 ± 70, 4,900 ± 70 and 3,440 ± 70 years BP, respectively. The faecal samples were processed and examined using paleoparasitological procedures. A total of 582 eggs of parasites were found in 47 coprolites. Samples were positive for eggs of Trichuris sp. (Nematoda: Trichuridae), Calodium sp., Eucoleus sp., Echinocoleus sp. and an unidentified capillariid (Nematoda: Capillariidae) and for eggs of Monoecocestus (Cestoda: Anoplocephalidae). Quantitative differences among layer for both coprolites and parasites were recorded. In this study, the specific filiations of parasites, their zoonotic importance, the rodent identity, on the basis of previous zooarchaeological knowledge, and the environmental conditions during the Holocene in the area are discussed.


Asunto(s)
Animales , Historia Antigua , Cestodos/aislamiento & purificación , Heces/parasitología , Fósiles , Helmintiasis Animal/historia , Nematodos/aislamiento & purificación , Roedores/parasitología , Argentina , Helmintiasis Animal/parasitología , Paleopatología , Recuento de Huevos de Parásitos
10.
Mem. Inst. Oswaldo Cruz ; 104(8): 1177-1180, Dec. 2009. ilus
Artículo en Inglés | LILACS | ID: lil-538179

RESUMEN

Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniensis (Coccidia) were recovered from faecal samples. The results obtained from the analysis provide evidence of consumption by felids of the viscera of both rodents and camelids. This knowledge allows for improved explanations as to the distribution of parasitism and its significance to the health of humans and animals inhabiting the area under study during the Middle Holocene.


Asunto(s)
Animales , Cestodos/aislamiento & purificación , Eimeria/aislamiento & purificación , Fósiles , Heces/parasitología , Felidae/parasitología , Nematodos/aislamiento & purificación , Argentina , Cestodos/clasificación , Eimeria/clasificación , Nematodos/clasificación , Paleopatología
11.
Mem Inst Oswaldo Cruz ; 104(8): 1177-80, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20140380

RESUMEN

Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniensis (Coccidia) were recovered from faecal samples. The results obtained from the analysis provide evidence of consumption by felids of the viscera of both rodents and camelids. This knowledge allows for improved explanations as to the distribution of parasitism and its significance to the health of humans and animals inhabiting the area under study during the Middle Holocene.


Asunto(s)
Cestodos/aislamiento & purificación , Eimeria/aislamiento & purificación , Heces/parasitología , Felidae/parasitología , Fósiles , Nematodos/aislamiento & purificación , Animales , Argentina , Cestodos/clasificación , Eimeria/clasificación , Nematodos/clasificación , Paleopatología
12.
Mem. Inst. Oswaldo Cruz ; 101(supl.2): 97-105, Dec. 2006.
Artículo en Inglés | LILACS | ID: lil-441361

RESUMEN

The objective of this paper is to present the first steps into the study of health in southern Patagonia during pre and post Native-European contact. Thus, our work has a double purpose. First, to discuss characteristics and relevance of human bone records of southern Patagonia, in order to study health in a population context. Second, to show some new lines of information, which include paleoparasitology, nutritional paleopathologies, and the study of lifestyles from human remains. In this context, we have started working on the first Spanish settlement "Nombre de Jesus", founded in 1584, and with historical documentation of "La Candelaria" Mission in Rio Grande (1896-1931).


Asunto(s)
Femenino , Historia del Siglo XVI , Historia del Siglo XVII , Historia del Siglo XVIII , Historia del Siglo XIX , Humanos , Masculino , Población Blanca , Indígenas Sudamericanos/historia , Relaciones Raciales/historia , Argentina , Colonialismo , Estado de Salud , Estilo de Vida , Paleopatología
13.
Mem Inst Oswaldo Cruz ; 101 Suppl 2: 97-105, 2006 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-17308816

RESUMEN

The objective of this paper is to present the first steps into the study of health in southern Patagonia during pre and post Native-European contact. Thus, our work has a double purpose. First, to discuss characteristics and relevance of human bone records of southern Patagonia, in order to study health in a population context. Second, to show some new lines of information, which include paleoparasitology, nutritional paleopathologies, and the study of lifestyles from human remains. In this context, we have started working on the first Spanish settlement "Nombre de Jesus", founded in 1584, and with historical documentation of "La Candelaria" Mission in Rio Grande (1896-1931).


Asunto(s)
Indígenas Sudamericanos/historia , Relaciones Raciales/historia , Población Blanca , Argentina , Colonialismo , Femenino , Estado de Salud , Historia del Siglo XVI , Historia del Siglo XVII , Historia del Siglo XVIII , Historia del Siglo XIX , Humanos , Estilo de Vida , Masculino , Paleopatología
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