RESUMEN
The cyst-forming protozoan parasite Neosporacaninum is one of the main causes of bovine abortion worldwide and is of great economic importance in the cattle industry. Recent studies have revealed extensive genetic variation among N. caninum isolates based on microsatellite sequences (MSs). MSs may be suitable molecular markers for inferring the diversity of parasite populations, molecular epidemiology and the basis for phenotypic variations in N. caninum, which have been poorly defined. In this study, we evaluated nine MS markers using a panel of 11 N. caninum-derived reference isolates from around the world and 96 N. caninum bovine clinical samples and one ovine clinical sample collected from four countries on two continents, including Spain, Argentina, Germany and Scotland, over a 10-year period. These markers were used as molecular tools to investigate the genetic diversity, geographic distribution and population structure of N. caninum. Multilocus microsatellite genotyping based on 7 loci demonstrated high levels of genetic diversity in the samples from all of the different countries, with 96 microsatellite multilocus genotypes (MLGs) identified from 108 N. caninum samples. Geographic sub-structuring was present in the country populations according to pairwise F(ST). Principal component analysis (PCA) and Neighbor Joining tree topologies also suggested MLG segregation partially associated with geographical origin. An analysis of the MLG relationships, using eBURST, confirmed that the close genetic relationship observed between the Spanish and Argentinean populations may be the result of parasite migration (i.e., the introduction of novel MLGs from Spain to South America) due to cattle movement. The eBURST relationships also revealed genetically different clusters associated with the abortion. The presence of linkage disequilibrium, the co-existence of specific MLGs to individual farms and eBURST MLG relationships suggest a predominant clonal propagation for Spanish N. caninum MLGs in cattle.
Asunto(s)
Bovinos/parasitología , Coccidiosis/parasitología , Variación Genética , Repeticiones de Microsatélite/genética , Neospora/genética , Animales , Argentina/epidemiología , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/parasitología , Coccidiosis/epidemiología , ADN Protozoario/análisis , Técnicas de Genotipaje , Geografía , Alemania/epidemiología , Neospora/aislamiento & purificación , Escocia/epidemiología , Ovinos/parasitología , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/parasitología , España/epidemiologíaRESUMEN
Objetive: to describe a patient with radicular pain secondary to a psoas muscle hematoma. Description: a 76 years old female, suffering from hypertension and medicated with anticoagulants after a triple coronary stent, complained of lumbar pain treated with analgesics and physiotherapy. The pain persisted and the condition worsened with the appearance of proximal crural paresia. An MRI showed an increased volume of the right psoas iliac muscle due to a hematoma. Intervention: anticoagulants were suspended and a neurological evaluation was done. The pain, paresia and hematoma receded. Conclusion: spontaneous hematoma of the psoas muscle is an infrequent cause of radicular syndrome although it should be taken into consideration in patients medicated with anticoagulants.
Asunto(s)
Hematoma , Músculos Psoas , RadiculopatíaRESUMEN
Objetivo: describir la frecuencia de observación de los signos descriptivos en neuroimágenes para la localización del surco central. Material y método: se emplearon imágenes por resonancia magnética (IRM) de 60 pacientes adultos (18 a 83 años), de ambos sexos (34 mujeres y 26 varones) con cerebros normales, en cortes axiales rostralesponderadas en secuencias T1 y T2, realzadas en resonador Phillips 1.5 Tesla. En las IRM se buscaron los siguientes signos: la unión surco frontal superior con el surco precentral, el signo del omega invertido, la impronta de la rama marginal del surco del cíngulo en la convexidad (pars Bracket), el surco post central que se bifurca en su porción superior, el giro post central más fino que el giro precentral, la unión surco intraparietal con el surco post central y el surco central que alcanza la línea media. Se determino su frecuencia. Resultados: el surco frontal superior se une con el surco precentral en 95%. El signo del omega invertido se evidencia en 96.66% de los casos. El signo de pars Bracket esta presente en 95% de las observaciones. El surco post central es bífido en 65%. El giro post central es más estrecho que el giro precentral en 98.33%. El surco intraparietal intercepta con el post central en 83.33%. El surco central alcanza la línea media en 86.66% de las observaciones. Conclusión: los signos descriptos tienen una alta frecuencia de presentación, encontrándose en promedio en 89.28% de las URM observadas...