RESUMEN

OBJECTIVE: It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease. METHODS: Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections. RESULTS: Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells. CONCLUSION: Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.

Asunto(s)

Galectina 3/sangre , Enfermedades Inflamatorias del Intestino/sangre , Animales , Biomarcadores , Western Blotting , Colitis/inducido químicamente , Colitis Ulcerosa/sangre , Colon/metabolismo , Enfermedad de Crohn/sangre , Sulfato de Dextran , Electroforesis en Gel de Poliacrilamida , Escherichia coli/metabolismo , Femenino , Fluoresceína-5-Isotiocianato , Colorantes Fluorescentes , Humanos , Inmunohistoquímica , Enfermedades Inflamatorias del Intestino/inducido químicamente , Enfermedades Inflamatorias del Intestino/diagnóstico , Lectinas/metabolismo , Receptores de Lipopolisacáridos/análisis , Receptores de Lipopolisacáridos/metabolismo , Ratones , Ratones Endogámicos BALB C