RESUMEN

Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.

Asunto(s)

Endocitosis/fisiología , Papillomavirus Humano 16/fisiología , Papillomavirus Humano 18/fisiología , Papillomavirus Humano 31/fisiología , Internalización del Virus , Actinas/metabolismo , Dinamina II , Electroforesis en Gel de Poliacrilamida , Células HeLa , Humanos , Proteínas de Membrana de los Lisosomas/metabolismo , Microdominios de Membrana , Microscopía Confocal , Microscopía Electrónica , Microscopía Fluorescente , Polimerizacion , Tetraspanina 24/metabolismo