RESUMEN
Correlative relations of the liver supernatant enzymatic activity of catalase (CAT) and glutathione-peroxidase (GP) with the velocities of oxygen consumption (VO2) and carbon dioxide exhalation (VCO2) were analyzed by the two- and three-dimensional linear and non-linear statistical methods in mice. It is shown that despite the close functions, CAT and GP, nevertheless, exhibit opposite correlative and regressive links with the gaseous exchange indices. As was found by the pairwise linear method, the activity correlation with VO2 was positive for CAT, but negative for GP. The opposite patterns of correlation were also apparent under the three-dimensional non-linear analysis.
Asunto(s)
Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Hígado/enzimología , Consumo de Oxígeno/fisiología , Animales , Dióxido de Carbono/metabolismo , Interpretación Estadística de Datos , Hígado/metabolismo , Ratones , Ratones Endogámicos CBA , Oxígeno/metabolismoRESUMEN
Qualitative and quantitative differences in correlative and regressive links between superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase were assessed in the mice liver by two- and three-dimensional statistical methods. Paired linear correlation analysis indicated SOD-CAT tandem as the correlatively acting enzymatic pair. Three-dimensional analysis revealed uniform response surfaces which exhibited higher activities at disproportional values of the other two and lower activities at proportional activities of the other two enzymes. The direct effect of the enzymes on each other was positive [table: see text] while the effect of their product was always negative.
Asunto(s)
Catalasa/metabolismo , Glutatión Peroxidasa/metabolismo , Hígado/enzimología , Superóxido Dismutasa/metabolismo , Animales , Modelos Lineales , Ratones , Ratones Endogámicos CBARESUMEN
Correlative and regressive relations between the gaseous exchange, thermoregulation and mitochondrial protein content were analyzed by two- and three-dimensional statistics in mice. It has been shown that the pair wise linear methods of analysis did not reveal any significant correlation between the parameters under exploration. However, it became evident at three-dimensional and non-linear plotting for which the coefficients of multivariable correlation reached and even exceeded 0.7-0.8. The calculations based on partial differentiation of the multivariable regression equations allow to conclude that at certain values of VO2, VCO2 and body temperature negative relations between the systems of gaseous exchange and thermoregulation become dominating.
Asunto(s)
Temperatura Corporal/fisiología , Mitocondrias Hepáticas/fisiología , Proteínas Mitocondriales/fisiología , Consumo de Oxígeno/fisiología , Animales , Femenino , Ratones , Ratones Endogámicos CBA , Mitocondrias Hepáticas/química , Análisis de RegresiónRESUMEN
In experiments on isolated rat heart, it has been shown that in old animals the sensitivity to restriction of the coronary perfusion increases whereas the resistance of the heart to this restriction decreases. It was established that incomplete reversible ischaemia causes significant changes in different steps of Ca2(+)-transporting system of the myocardium which are more evident in older animals and which presumably account for the increased sensitivity of cardiac muscle to ischaemia.
Asunto(s)
Envejecimiento/fisiología , Calcio/metabolismo , Circulación Coronaria/fisiología , Contracción Miocárdica/fisiología , Miocardio/metabolismo , Animales , Transporte Biológico/fisiología , Técnicas In Vitro , Perfusión/instrumentación , Perfusión/métodos , Ratas , Ratas EndogámicasRESUMEN
To study the age-related relationship between the membrane status and activity of membrane-linked enzymes, the physicochemical properties of plasma membranes and activity of transfer ATPase and adenylate cyclase were assessed in tissues of rats of different ages. The lipid matrix of membranes was found to undergo appropriate changes with age. The most significant shifts are manifested under functional and metabolic stresses. It has been demonstrated that the shifts in the regulated activity of the studied enzymes are induced by changes in membrane interactions. The findings suggest predominantly the membrane mechanisms that determine the insufficiency of adaptation mechanisms in old age.
Asunto(s)
Membrana Celular/análisis , Lípidos/análisis , Adaptación Fisiológica , Adenosina Trifosfatasas/análisis , Adenilil Ciclasas/análisis , Factores de Edad , Animales , Química Encefálica , Colesterol/análisis , Hepatectomía , Hígado/análisis , Miocardio/análisis , Fosfolípidos/análisis , RatasAsunto(s)
Envejecimiento/fisiología , Circulación Pulmonar , Adulto , Anciano , Femenino , Hemodinámica , Humanos , Masculino , Persona de Mediana Edad , Caracteres SexualesRESUMEN
Experiments with different species of animals and different models (in situ heart, isolated perfused heart, isolated papillary muscle) revealed a reduction of functional capacity of ageing heart. A decrease of Ca2+ uptake by sarcoplasmic reticulum and mitochondria and diverse age-dependent shifts were found in sarcolemma. The data obtained suggest that the development of the cardiac contractile function disorders in ageing largely depends of the age-related changes in the Ca2+ transport system.
Asunto(s)
Envejecimiento/fisiología , Calcio/metabolismo , Contracción Miocárdica , Miocardio/metabolismo , Adenosina Trifosfatasas/metabolismo , Animales , Transporte Biológico , Gatos , Perros , Estimulación Eléctrica , Hemodinámica , Técnicas In Vitro , Músculos Papilares/fisiología , Conejos , Ratas , Sodio/metabolismoRESUMEN
Renal function of 91 healthy subjects aged 18-91 has been evaluated at radionuclide renography with the use of 131I-Hippuran. Renal excretory function, effective renal plasma and blood flows were found to decrease annually by 0.09 ml/kg in ageing men and by 0.08 ml/kg in women. The use of new indices may be useful for evaluation of renal blood circulation, especially during hypo- or hypervolaemia is proposed. These are the index of renal circulation efficiency and the mean time of circulating blood volume transfer through renal blood vessels.
Asunto(s)
Renografía por Radioisótopo , Adolescente , Adulto , Factores de Edad , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Valores de Referencia , Factores SexualesRESUMEN
A study was made of the central hemodynamics in practically healthy persons aged 20 to 90 using radiocardiography. The results have shown that cardiac discharge decreases regularly with age, the time of the greater circulation and general peripheral resistance increase, the volume of the circulating pulmonary blood decreases in the presence of its practically unchanged general volume. The authors present data on the main indices of the central hemodynamics in men and women of various age groups.
Asunto(s)
Envejecimiento , Corazón/diagnóstico por imagen , Hemodinámica , Adulto , Anciano , Tiempo de Circulación Sanguínea , Determinación del Volumen Sanguíneo , Gasto Cardíaco , Femenino , Corazón/fisiología , Humanos , Radioisótopos de Yodo , Masculino , Persona de Mediana Edad , Cintigrafía , Valores de ReferenciaRESUMEN
A study was made of the muscular blood flow at rest (MBFR) and in reactive hyperemia (MMBF--maximum muscular blood flow) in 170 healthy persons using a method of local clearance with 133Xe. The results of the study showed a regular decrease in the MBFR and MMBF level in aging, the decrease rates of MMBF considerably prevailing over those of MBFR. In the age interval between 18-96 MBFR lessens by 24%, MMBF more than twice. The age-associated time course of MMBF is of a more complicated nature as compared to that of MBRF and indicates a considerable reduction of the functional reserves of the tissue blood flow in the skeletal muscles of middle-aged and old persons. The above data make it possible to outline MBFR rough standard values: for young persons--2.5-5.0 ml/100 g/min., for older persons--1.5-3.5 ml/100 g/min. As normal MBFR fluctuations in healthy persons one can adopt a +/- 20% interval of respective MBRF mean values in each decade. MBF age-associated changes should be regarded in the diagnosis of obliterating lesions of lower limb vessels and other diseases accompanied by its decrease. Special emphasis should be placed on MMBF.