RESUMEN
BACKGROUND: On the basis of available reports it can be stated that physical stress causes changes in distribution and activity of many components of the immune system. It is believed that psychophysical stress in soldiers can influence their immune system depressively and in consequence increase the risk of upper respiratory tract infections. Therefore, it was decided to conduct studies aimed at the estimation of the influence of military training on the some parameters of cellular immune response. MATERIAL AND METHODS: The study group consisted of 40 draft aged from 18 to 23 years. The research was conducted in the first 8 weeks of service, in the period of the most intense draft stress adaptation. The participants were divide into 2 groups, A and B respectively, 20 soldiers each. Group A derived from an assault unit. Their training induced strenuous physical stress. Group B derived from a support unit. Their training required less physical effort then one of group A. Performed examinations involved: lymphocyte percentage count, lymphocyte proliferative response to mitogen, CD69 antigen expression on T lymphocyte surface, delayed hypersensitivity reaction with CMI Multitest. All assessments were done twice at 8 weeks interval. RESULTS: After 8 weeks of training in the A group a statistically significant increase in the percentage of lymphocytes revealing antigens of the II Class Main Histocompatibility Complex (MHC) was found. In addition, in this group a statistically significant decrease in the value of lymphocyte stimulation index, a statistically significant increase in the percentage of cells revealing CD69 antigen expression after PHA stimulation were observed. During investigated period in the B group following statistically significant changes were found: an increase in the percentage of CD3+ and CD4+ cells, a decrease in the percentage of CD16+CD56+ and an increase in the CD4+ to CD8+ ratio. CONCLUSION: The obtained results show that military service conditions influence some parameters of the cellular immune response but do not result in the clinically significant suppression of the immune system.
Asunto(s)
Personal Militar , Infecciones del Sistema Respiratorio/inmunología , Estrés Fisiológico , Adolescente , Adulto , Antígenos CD/sangre , Antígenos de Diferenciación de Linfocitos T/sangre , Complejo CD3/sangre , Antígenos CD4/sangre , Antígeno CD56/sangre , Antígenos CD8/sangre , Genes MHC Clase II , Humanos , Células Asesinas Naturales/inmunología , Lectinas Tipo C , Linfocitos/inmunología , Linfocitos/metabolismo , Complejo Mayor de Histocompatibilidad , Receptores de IgG/sangreRESUMEN
It is known, that psychophysical stress causes some changes in distribution and activity of many components of the immune system. The research was aimed at examination the influence of different military service conditions on some neutrophil functions. In this study granulocyte chemiluminescence both without stimulation and after stimulation with fMLP, PMA and zymosan was evaluated. In addition, the expression of surface adhesion molecules--CD11b on circulating granulocytes was examined. The studied group consisted of 20 soldiers from an assault unit (group A) and 20 soldiers from a support unit (group B), the age ranged from 18 to 23 years. The examinations were conducted at the beginning of the basic training and after the 8 weeks period. In group A the following results were obtained: a significant increase (p < 0.001) in granulocyte chemiluminescence without stimulation and after stimulation with fMLP, PMA and zymosan, as well as, a significant decrease (p < 0.05) in CD11b expression on granulocytes. And in group B: a significant increase (p < 0.05) in granulocyte chemiluminescence without stimulation but a significant decrease (p < 0.05) after cells stimulation with fMLP and PMA were observed. In this group CD11b expression on granulocytes was significantly (p < 0.001) decreased. The results of the present examination indicate, that military service conditions influence some parameters of the innate immunity.
Asunto(s)
Antígeno de Macrófago-1/análisis , Personal Militar , Neutrófilos/inmunología , Estrés Fisiológico/inmunología , Adulto , Humanos , Masculino , Polonia , Estrés Psicológico/inmunologíaRESUMEN
Peripheral venous blood leukocyte subpopulations were determined in 69 healthy children and compared in two age groups: 8 to 12 years and 13 to 17 years, the period of pubescence and intensive growth of the organism. As compared to work of other authors, more leukocyte subpopulations were recognized in the examined large group of children. Whole blood monocyte and total lymphocyte absolute counts decreased with age as well as the absolute counts of T lymphocytes and CD8+ T lymphocytes. Percentage of CD4+ cells and CD4/CD8 ratio were higher in the older age group. Percentage as well as absolute counts of CD19+ CD5+ cells and CD19+ CD10+ cells are given.
Asunto(s)
Leucocitos/citología , Subgrupos Linfocitarios/citología , Adolescente , Factores de Edad , Antígenos CD/análisis , Recuento de Células , Niño , Femenino , Citometría de Flujo , Humanos , Leucocitos/química , Subgrupos Linfocitarios/química , Masculino , Factores Sexuales , Linfocitos T/citologíaRESUMEN
The studies were performed on healthy well-trained cyclists. Maximal physical exercise was performed on a Monark bicycle ergometer according to individual schemes. Heart rate amounting to about 200 bts/min and oxygen consumption stabilization were considered as criteria for maximal physical exercise. In this study we have investigated the effect of short-term stimulation of conditioned sportsmen with thymic hormones and evaluated T cell subsets, DR antigen and transferrin receptor expression as well as mitogen-induced proliferation of lymphocytes before and after maximal physical effort. The results suggest that intensive physical exercise may be responsible for transient decrease of CD4/CD8 ratio and mitogen responsiveness, and increase of mononuclear cells number bearing HLA DR+ and CD71 antigens. These changes were modified by the treatment with thymic hormones.