RESUMEN
OBJECTIVE: To assess quality of life of children and teenagers with recurrent respiratory papillomatosis, according to the evidence of infection by human papillomavirus types 6 and 11, compared with healthy volunteers and patients with chronic otitis media. METHOD: Participants and their parents completed the Pediatric Quality of Life Inventory 4.0. RESULTS: Patients with recurrent respiratory papillomatosis and their parents reported lower quality of life than healthy subjects (p < 0.01), but similar quality of life to patients with chronic otitis media. Those with human papillomavirus type 11 showed the lowest scores among all participants (p < 0.05). CONCLUSION: Young Mexican patients with recurrent respiratory papillomatosis and their parents perceive a poor quality of life, and they may experience limitations in interactions with their peers. Infection by human papillomavirus type 11 may increase the impact of the disease on quality of life.
Asunto(s)
Infecciones por Papillomavirus/psicología , Calidad de Vida , Infecciones del Sistema Respiratorio/psicología , Adolescente , Estudios de Casos y Controles , Niño , Enfermedad Crónica , Femenino , Papillomavirus Humano 11 , Papillomavirus Humano 6 , Humanos , Masculino , México , Recurrencia Local de Neoplasia/psicología , Otitis Media/psicología , Infecciones por Papillomavirus/virología , Padres/psicología , Recurrencia , Infecciones del Sistema Respiratorio/virología , Encuestas y CuestionariosRESUMEN
Cefodizime has modulating effects on the release of diverse cytokines. We determined the modulator activity of this antibiotic on the production of TNF in human monocytic U-937 cells. The measurement of TNF was carried out by ELISA test and by a L-929 cells-based citotoxic bioassay. The results showed that cefodizime alone induced the production of TNF on U-937 cells, however, the addition of LPS led to a decrease in the release of this cytokine (p < 0.05). On the other hand, the combination of cefodizime-PMA had a synergic effect (p < 0.05), while addition of LPS to this combination caused a decrease of TNF production (p < 0.05). With these results we conclude that cefodizime modulates the production of TNF in U-937 cells, which is down regulated by the addition of LPS.
Asunto(s)
Cefotaxima/farmacología , Cefalosporinas/farmacología , Monocitos/efectos de los fármacos , Factor de Necrosis Tumoral alfa/biosíntesis , Cefotaxima/análogos & derivados , Línea Celular , Humanos , Lipopolisacáridos/farmacología , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Tumor necrosis factor (TNF-alpha) is a cytokine which can be found in peritoneal fluid (PF) of patients with endometriosis and pelvic inflammatory disease (PID) as a response of inflammatory disorder and infections diseases. The cytotoxic effect of this cytokine could be participating in the pathology of different gynecologic problem and be accountable of the high immunological response and damage on the tubal epithelium. The objective of this study was determinate the presence of TNF-alpha in PF of endometriosis patients, fallopian tube occlusion (FTO) and PID and their correlation with different isolated bacteria. Ten mililiter PF were collected and cultured in antificial medium and Mc Coy culture cells for isolation of acrobic, and anaerobic bacteria and Chlamydia trachomatis from 73 patients by laparoscopy. The TNF-alpha activity was determined by L-929 cells endometriosis, 30 PID and 4 had miomas and adherences. The 50.7% of patients were cultive positive, fom these, 31.5% were PID. Chlamydia trachomatis (16%) was the most frecuenty isolated bacteria in these patients. 59.4% of FTO patients displayed TNF-alpha activity. However, only 4% showed positive isolation, in conclusion the detection of TNF-alpha could be useful in active infectious and inflammatory diseases in patients which not present simptomatologic characteristic of these illnesses and plus being attended at for sterility clinical as a result of their incapacity to get pregnant.
Asunto(s)
Líquido Ascítico/microbiología , Endometriosis/patología , Infertilidad Femenina/etiología , Enfermedad Inflamatoria Pélvica/patología , Factor de Necrosis Tumoral alfa/análisis , Líquido Ascítico/química , Citotoxicidad Inmunológica , Endometriosis/microbiología , Femenino , Humanos , Laparoscopía , Enfermedad Inflamatoria Pélvica/microbiología , EmbarazoRESUMEN
The prevalence of C. trachomatis in neonates with respiratory distress was studied after 24 h of birth, nine patients were positive for C. trachomatis culture (12.9%). The chest radiographs showed six with hyaline membrane disease and two with pneumonia. One patient with treatment of ventilation mechanics developed bronchopulmonary dysplasia and was C. trachomatis positive in a second cell culture. Of the nine patients with C. trachomatis, eight were neonates preterm with low weight to the birth and with leukocytosis. Six patients were delivered by cesarean section. These results suggest that C. trachomatis can participate in an important way in the development of the distress respiratory in infants preterm.
Asunto(s)
Infecciones por Chlamydia/epidemiología , Chlamydia trachomatis/aislamiento & purificación , Trastornos Respiratorios/etiología , Adulto , Bacterias/aislamiento & purificación , Infecciones Bacterianas/complicaciones , Infecciones Bacterianas/congénito , Infecciones Bacterianas/epidemiología , Líquido del Lavado Bronquioalveolar/microbiología , Cesárea , Infecciones por Chlamydia/complicaciones , Infecciones por Chlamydia/congénito , Infecciones por Chlamydia/transmisión , Femenino , Enfermedades Fetales/microbiología , Humanos , Recién Nacido de Bajo Peso , Recién Nacido , Recien Nacido Prematuro , Masculino , México/epidemiología , Embarazo , Complicaciones Infecciosas del Embarazo , Prevalencia , Trastornos Respiratorios/congénito , Trastornos Respiratorios/microbiología , Trastornos Respiratorios/virología , Infecciones por Virus Sincitial Respiratorio/complicaciones , Infecciones por Virus Sincitial Respiratorio/congénito , Infecciones por Virus Sincitial Respiratorio/epidemiología , Virus Sincitiales Respiratorios/aislamiento & purificaciónRESUMEN
The sensitivity, specificity, positive and negative predictive values and positive and negative false percentage of three monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections were evaluated, 150 cervical specimens and 50 samples of peritoneal liquid of patients with pelvic inflammatory disease, were studied. The results showed that the reagents against the main protein of external membrane of Chlamydia introduced high sensitivity but minor specificity than the reagent of immunofluorescence direct against then chlamydial lipopolysaccharide.