RESUMEN

"Light" viral antigen (HAAg) with buoyant density 1.20 g/cm2 and sedimentation coefficient 92S are accumulated together with mature viral particles in Hepatitis A virus (HAV) infected FRhK-4 cells. This HAAg is localized predominantly in endoplasmic reticulum fraction of infected cells, while nature virions are localized in cytosol. In contrast to mature virus, "light" HAAg is sensitive to trypsin digestion and is not able to hybridize with synthetic oligodeoxinucleotide which is complementary to structural part of HAV RNA. Antigenic properties of mature virus and "light" antigen are compared.

Asunto(s)

Hepatovirus/patogenicidad , Animales , Antígenos Virales/análisis , Fenómenos Biofísicos , Biofisica , Membrana Celular/microbiología , Hepatitis A/inmunología , Hepatitis A/microbiología , Hepatovirus/inmunología , Hepatovirus/fisiología , Péptidos/análisis , ARN Viral/análisis , Virión/inmunología , Virión/patogenicidad , Virión/fisiología , Cultivo de Virus , Replicación Viral