RESUMEN
A method was proposed for calculating the content of intracellular components during the cell cycle of an individual cell. The principle of reverse problems was used in the mathematical model proposed. The model allowed us to calculate changes of intracellular parameters of an individual cell from corresponding parameters measured in the whole culture. Optical density, total SH-group and glutathione content in synchronous culture of E. coli were the parameters studied. The proposed method may be applied for both synchronous and asynchronous cell cultures.
Asunto(s)
Ciclo Celular , Escherichia coli/citología , Glutatión/metabolismo , Compuestos de Sulfhidrilo/metabolismo , Escherichia coli/metabolismo , Modelos Teóricos , Óptica y FotónicaRESUMEN
Phospholipid concentrations have been estimated throughout the successive cell cycle in synchronously growing culture of E. coli B/r. Total phospholipid phosphorus was shown to be doubled in the period of time between two cell divisions, whereas during the division itself it did not change. Phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) exhibit a stepwise increase during the cell cycle. It should be noted that the phase of accumulation of these lipids could shift depending on the duration of the cell cycle. The fall in level of PE was followed by a short-term increase (5-10 min). At the same time the level of cardiolipin was observed to be significantly increased.