RESUMEN

In this study, the complete genome of an isolate of yam mild mosaic virus (YMMV) from Brazil was sequenced, and the predicted amino acid sequence was analyzed. The YMMV RNA genome consists of 9538 nt without the poly(A) tail, encoding a putative typical potyvirus polyprotein of 3084 amino acids. Furthermore, the small overlapping ORF (PIPO) in the P3 gene was also deduced, and the cleavage sites of the polyprotein were predicted. Multiple alignment with other potyviruses showed a maximum nucleotide sequence identity of 64 % to wild tomato mosaic virus. A phylogenetic tree showed that YMMV clustered with Asian potyviruses that mainly infect solanaceous plants.

Asunto(s)

Genoma Viral , Potyvirus/genética , ARN Viral/genética , Análisis de Secuencia de ADN , Brasil , Análisis por Conglomerados , Dioscorea/virología , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Filogenia , Enfermedades de las Plantas/virología , Potyvirus/aislamiento & purificación , Homología de Secuencia de Ácido Nucleico