Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 94
Filtrar
1.
Phys Rev Lett ; 129(9): 094501, 2022 Aug 26.
Artículo en Inglés | MEDLINE | ID: mdl-36083645

RESUMEN

We demonstrate, experimentally and by molecular dynamics simulations, that krypton and xenon form nanostructured water-gas domains. High pressure was applied to force the inert gases to dissolve in water following Henry's law, then the liquid was depressurized, centrifuged, and inspected by dynamic light scattering. The observed objects have similar sizes and electrical properties to nanobubbles, but we found that they have fairly neutral buoyancy even at high gravitational fields. We posit that the formed nano objects are not bubbles but blobs, unique structures conceived as clathrate-hydrate precursors, thus resolving the so-called Laplace pressure bubble catastrophe.

2.
Climacteric ; 24(3): 236-245, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-33733982

RESUMEN

Hormone replacement therapy in menopause is used to improve climacteric syndrome in women whose quality of life is affected. However, given the wide variety of progestogens available, it is important to evaluate their differential benign changes (radiological, cellular, and clinical) on the breast. This review aimed to determine the different benign changes of progestogens used in postmenopausal combined hormone therapy on the breast (radiological, cellular, and clinical), in women without mammary pathology, in order to establish their safety profile. A systematic review of the literature was carried out with a balanced search strategy for the identification of relevant references in the MEDLINE, BVSalud, EMBASE, ProQuest, and Cochrane databases until November 2019. The search terms used were 'menopause' or 'hormonal replacement therapy' or 'progestins' or 'estrogen' or 'mastodynia' or 'benign breast disease' or 'mammography'. Data were collected from the 'eligible' articles by two researchers (ARF and SHA), and possible discrepancies in inclusion were resolved by consensus. A total of 1886 articles were identified; 60 full-text articles were reviewed, and 17 articles that met the inclusion criteria were included for the qualitative analysis. In conclusion, combined hormone replacement therapy is associated with benign effects on the breast, such as mastodynia and increased mammographic density.


Asunto(s)
Enfermedades de la Mama/inducido químicamente , Mama/efectos de los fármacos , Terapia de Reemplazo de Estrógeno/efectos adversos , Posmenopausia/efectos de los fármacos , Progestinas/efectos adversos , Densidad de la Mama/efectos de los fármacos , Femenino , Humanos , Persona de Mediana Edad
3.
Mar Pollut Bull ; 165: 112153, 2021 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-33607451

RESUMEN

This study evaluated mercury (Hg) in three coastal areas in the North Atlantic Ocean (Azores archipelago, Northwest Portugal and Southeast Mexico) and calculated their enrichment factor and geoaccumulation index. Hg presented greater affinity with fine sediment fraction. In all sampling areas the sites with proximity to possible Hg hotspots presented increased [Hg] in fine sediments. However, this fraction only represents less than 1% of the whole sediment, resulting in a significantly lower [Hg] in the total sediment fraction. After comparing [Hg] in the fine fraction and the values established by the Sediment Quality Guidelines, two sites in the Azores exceeded these values. Nevertheless, when considering the total sediment fraction, adverse biological effects are not expected to occur. Finally, both enrichment factor and geoaccumulation index indicated that the Hg present in 86% comes from natural sources and only sampling sites close to potential hotspots showed some degree of enrichment and contamination.


Asunto(s)
Mercurio , Contaminantes Químicos del Agua , Océano Atlántico , Azores , Monitoreo del Ambiente , Sedimentos Geológicos , Mercurio/análisis , México , Portugal , Contaminantes Químicos del Agua/análisis
4.
Helminthologia ; 58(4): 403-407, 2021 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-35095317

RESUMEN

From January to May 2015, a sample of 50 individuals of the Pacific sierra Scomberomorus sierra Jordan and Starks, 1895 captured off Mazatlán (southeastern Gulf of California, Mexico) were reviewed for helminths. A total of 6, 255 parasitic worms belonging to 11 species (9 in adult stage and 2 larvae) were obtained. Trematoda was the best represented group with 6 species of Didymozoidae Monticelli, 1888 and 1 of Bucephalidae Poche, 1907. In addition, 2 monogenean species and 2 of Nematoda were collected. In this study, the first molecular sequences for didymozoid species in Mexico were generated, and for Glomeritrema sp. at worldwide level. The most prevalent species were Didymocylindrus sp. (92 %) and Didymocystis scomberomori (MacCallum & MacCallum, 1916) (88 %), whereas the monogenean Thoracocotyle crocea MacCallum, 1913 reached the highest value of mean intensity (75.2). The coincidence between the helminthological composition established in our study and that reported for the same scombrid in 4 localities from the Mexican South Pacific (sharing 10 species) suggests that this group of species persistently parasitize S. sierra throughout its distribution along the Mexican Pacific coast; furthermore, due to the richness of didymozoids and the affinity of Thoracocotylidae species for S. sierra, both groups can be considered typical parasites of this fi sh.

5.
Rev. argent. dermatol ; Rev. argent. dermatol;101(4): 101-110, dic. 2020. graf
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1288192

RESUMEN

RESUMEN Antecedentes: La pitiriasis rubra pilaris es una enfermedad infrecuente pápulo-escamosa crónica en la que existe un trastorno de la queratinización de la epidermis, caracterizada por pápulas foliculares hiperqueratósicas con tendencia a formar placas de coloración asalmonada, descamativas, con islas de piel sana asociadas a queratodermia palmo plantar. Presenta una distribución bimodal en la primera y sexta década de la vida, afectando a ambos sexos por igual. Su etiopatogenia es desconocida, se ha postulado una respuesta inmune anormal ante diferentes estímulos antigénicos, así como alteración del metabolismo de la vitamina A. Se ha clasificado en 6 tipos en base a su presentación, edad de inicio, curso y pronóstico, tratándose en forma tópica o sistémica. Casos clínicos: Se presentan dos casos en pacientes de 10 y 2 años de edad, con manifestaciones clínicas correspondientes al tipo juvenil circunscrito, que es el más frecuente en edad pediátrica y juvenil clásico respectivamente, con histopatología compatible y excelente respuesta al tratamiento tópico. Conclusiones: Aunque la pitiriasis rubra pilaris es una patología rara, deberá ser sospechada si el cuadro clínico es sugestivo, y si es compatible su histopatología. Debe considerarse que el tratamientotópico puede ser suficiente para lograr la resolución del cuadro.


ABSTRACT Background: Pityriasis rubra pilaris is an infrequent chronic papulosquamous disease in which there is a disorder of keratinization of the epidermis, characterized by hyperkeratotic follicular papules with a tendency to form salmon-colored, scaly plaques, with islands of healthy skin associated with palmoplantar keratoderma. It presents a bimodal distribution in the first and sixth decades of life, affecting both sexes equally. Its etiopathogenesis is unknown, an abnormal immune response has been postulated to different antigenic stimuli, as well as alteration of the metabolism of vitamin A. It has been classified into 6 types based on its presentation, age of onset, course and prognosis, being treated appropriately topical or systemic. Clinical cases: Two cases of 10 and 2 years of age are presented, with clinical manifestations corresponding to the circumscribed juvenile type, which is the most frequent in pediatric and classic juvenile age respectively, with compatible histopathology and excellent response to topical treatment. Conclusions: Although pityriasis rubra pilaris is a rare pathology, it should be suspected if the clinical picture is suggestive and its histopathology is compatible. It should be considered that topical treatment may be sufficient to achieve resolution of the condition.

6.
Soft Matter ; 16(42): 9655-9661, 2020 Nov 04.
Artículo en Inglés | MEDLINE | ID: mdl-33078812

RESUMEN

Combined coarse-grained (CG) and atomistic molecular dynamics (MD) simulations were performed to study the interactions of xenon with model lipid rafts consisting of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) and cholesterol (Chol). At a concentration of 2 Xe/lipid we observed an unexpected result: spontaneous nucleation of Xe nano bubbles which rapidly plunged into the bilayer. In this process Chol, essential for raft stabilization, was pulled out from the raft into the hydrophobic zone. When concentration was further increased (3 Xe/lipid), the bubbles increase in size and disrupted both the membrane and raft. We computed the radial distribution functions, pair-wise potentials, second virial coefficients and Schlitter entropy to scrutinize the nature of the interactions. Our findings, concurring with a recent report on the origin of general anaesthesia (M. A. Pavel, E. N. Petersen, H. Wang, R. A. Lerner and S. B. Hansen, Proc. Natl. Acad. Sci. U. S. A., 2020, 117(24), 13757-13766), suggest that the well-known anaesthetic effect of Xe could be mediated by sequestration of Chol, which, in turn, compromises the stability of rafts where specialized proteins needed to produce the nervous signal are anchored.


Asunto(s)
Membrana Dobles de Lípidos , Fosfatidilcolinas , Colesterol , Microdominios de Membrana , Xenón
7.
Int J Tuberc Lung Dis ; 24(7): 694-699, 2020 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-32718402

RESUMEN

BACKGROUND: In 2016, 3% of newly diagnosed patients with tuberculosis (TB) left the United States, of whom 24% moved to Mexico. Continuity of care for TB is important to ensure patients complete treatment and reduce TB transmission. CureTB provides continuity of care for patients with TB who move out of the United States by referring them for care at their destination.METHODS: Analysis of CureTB data collected between January 2012 to December 2015 to describe demographics and outcomes of referred patients and examine factors contributing to successful treatment outcomes.RESULTS: CureTB received 1347 referrals mostly from health departments and law enforcement agencies in the United States (92%). A total of 858 referrals were for patients with verified or possible TB (64%). Most patients moved to Mexico or other Latin American countries (96%) and completed treatment after departing (78%). Poor treatment outcomes were associated with being in custody (33%), not being interviewed by CureTB (30%), and not having diabetes (18%).CONCLUSION: CureTB successfully promoted transnational continuity of care for patients by exchanging information with international public health authorities and linking them directly with patients. This patient-centered strategy helps improve TB treatment success and reduce the global burden and transmission of TB.


Asunto(s)
Tuberculosis , Continuidad de la Atención al Paciente , Humanos , México , Derivación y Consulta , Resultado del Tratamiento , Tuberculosis/terapia , Estados Unidos
8.
ACS Omega ; 5(28): 17304-17313, 2020 Jul 21.
Artículo en Inglés | MEDLINE | ID: mdl-32715215

RESUMEN

Calcium scaling is a serious problem encountered in the oil and gas industry because it is common that brines produced alongside oil and gas exhibit high concentrations of calcium ions, among others, which is expensive to remedy. The precipitation of calcium salts on the internal wall of the pipelines may occur because of the physical and chemical changes as fluids are produced from downhole to surface facilities. Although different researchers have address scaling and corrosion in the oil and gas industry, there are few reports in the literature relating the corrosion and scaling phenomena simultaneously. Despite there being indications that scales may produce corrosion problems, affecting the mechanical integrity of the infrastructure, there is minimal research in the literature addressing such relations. Previous studies presented aluminum alloys as excellent and reliable materials for applications in the petroleum industry, such as drilling activities. In this work, we evaluate the corrosion behavior of steel and aluminum alloys under highly scaling environments using supersaturated brines. Our results show that the presence of calcium carbonate and calcium sulfate as a scaling environment increases the corrosion rates for aluminum alloys and carbon steel; however, the same environments do not affect the corrosion behavior of stainless steel.

9.
Mitochondrial DNA B Resour ; 5(3): 3310-3312, 2020 Sep 08.
Artículo en Inglés | MEDLINE | ID: mdl-33458148

RESUMEN

Here, we present the mitogenome of the blood feeding leech Haementeria acuecueyetzin (Hirudinida: Glossiphoniidae) based on specimens collected in Tabasco, Mexico. The circular genome is 14,985 bp in length, and consists of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and an AT-rich control region. Phylogenetic analysis based on the 13 protein-coding genes and two rRNA genes places H. acuecueyetzin sister to H. officinalis within the family Glossiphoniidae. Mitochondrial gene order in H. acuecueyetzin is consistent with other members of Clitellata with no evidence of gene gain/loss, duplication, or rearrangement.

10.
Mitochondrial DNA A DNA Mapp Seq Anal ; 30(4): 632-642, 2019 05.
Artículo en Inglés | MEDLINE | ID: mdl-31072187

RESUMEN

Genetic barcodes (partial sequences of the mitochondrial cytochrome c oxidase subunit I) were generated for freshwater leeches that inhabit the Mexico Basin, upon which Mexico City and its metropolitan area have developed. Once a continuous lake, the basin has passed through continuous events of artificial desiccation in the last 500 years so that it is currently conformed by a few and highly modified and polluted isolated freshwater bodies. Six species of leeches from three families were collected in five localities. Current sequence databases were able to determine five of the six species collected for this study with the only exception of Haemopis caballeroi, for which no sequence data are available in public repositories. Taxonomic assignment of cocoons was possible via comparison of barcode sequences. We discuss the presence of a population of Erpobdella ochoterenai in Tecocomulco Lake that bares high genetic divergence from its conspecifics, which may indicate it is an undescribed species.


Asunto(s)
Código de Barras del ADN Taxonómico , Lagos , Sanguijuelas/genética , Animales , Genoma Mitocondrial/genética , México , Especificidad de la Especie
11.
Int J Legal Med ; 133(3): 781-783, 2019 May.
Artículo en Inglés | MEDLINE | ID: mdl-30334083

RESUMEN

Mexican Mestizos (admixed) have been poorly studied for short tandem repeats (STRs) included for new human identification (HID) kits, such as the GlobalFiler PCR Amplification kit. Therefore, this kit was analyzed in 784 unrelated volunteers from the city of Tijuana (n = 381) and Sonora state (n = 403) in the northwest region of Mexico. Allele frequencies, forensic parameters, Hardy-Weinberg equilibrium, and linkage equilibrium were estimated or evaluated for 21 autosomal STRs, respectively. For this HID kit, the combined power of discrimination (PD) was > 0.99999999999999 (RMP range = 1.23 to 3.0 × 10-25), and the combined power of exclusion (PE) were 0.999999993 and 0.999999997 in Tijuana city and Sonora state, respectively. Interpopulation analyses based on STRs of the GlobalFiler kit was performed, including four Mexican Native American, one Mexican Mestizo, and four ethnic American populations (USA), previously studied. The low-but significant-differentiation observed among Mexican Mestizos (FST = 0.0969%; p = 0.02584) justifies the creation of STR databases for HID purposes in this country. In brief, results allow the confident use of the GlobalFiler kit for HID purposes in Mestizo population from the Northwest region Mexico.


Asunto(s)
Genética de Población , Repeticiones de Microsatélite , Dermatoglifia del ADN , Frecuencia de los Genes , Humanos , México , Reacción en Cadena de la Polimerasa
12.
Int. j. morphol ; 37(1): 174-177, 2019. tab, graf
Artículo en Español | LILACS | ID: biblio-990023

RESUMEN

RESUMEN: Las variantes anatómicas del tronco celíaco (TC) son relevantes en el ámbito quirúrgico e intervencionista. Existen diferentes estudios a nivel mundial que han demostrado las variaciones que puede presentar el TC tanto en longitud como en estructura; dichos estudios han sido realizados predominantemente en población asiática y europea. Por lo anterior, realizamos un estudio que caracterizó esta estructura y que sea referente para la población mexicana. Se analizó una muestra de 50 especímenes cadavéricos humanos embalsamados de origen mexicano. El promedio de longitud del TC a su primera rama fue de 12,44 mm; de su origen a la segunda rama fue de 17,07 mm; y hasta la tercera fue de 19,15 mm. En la muestra de estudio se encontraron variantes en el 20 % de los especímenes, de éstos el 14 % presentaron variantes morfométricas en cuanto a longitud y 6 % en estructura. Respecto a las variantes morfométricas, destacó la presencia de un TC de 3 mm de longitud. En cuanto a las variantes morfológicas dos individuos presentaron un tronco gastro-esplénico con tronco hepato-mesentérico; y el tercero un tronco bifurcado hepato-gástrico con la arteria esplénica naciendo de la arteria hepática común. Éste último no reportado en la literatura.


SUMMARY: Anatomical variants in the celiac trunk (CT) are important in surgical and interventional fields. Studies worldwide have demonstrated length and structure variations in the celiac trunk. These studies have predominantly been carried out in Asian and European population. Therefore, we considered it necessary to realize a study to describe this structure and serve as a reference in the Mexican population. A sample of 50 human cadaveric specimens of Mexican origin was analyzed. The average length of the celiac trunk from its origin to its first branch was 12.44 mm, to the second branch was 17.07 mm, and to the third branch was 19.15 mm. In the studied sample, variants were found in 20 % of the specimens, 14 % of these presented morphometric variations in terms of length, and 6 % in terms of structure. In reference to the morphometric variants, the presence of one 3 mm length CT was noteworthy. Morphological variants were found in two specimens presenting a gastro-splenic trunk with a hepatomesenteric trunk, and a third specimen with hepatogastric bifurcated trunk, with the splenic artery originating from the common hepatic artery was observed. No reports were found in the literature concerning the latter.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Arteria Celíaca/anatomía & histología , Variación Anatómica , Cadáver , México
13.
Artículo en Español | BDNPAR | ID: biblio-1047129

RESUMEN

Introducción: La población indígena, del estado Bolívar, habita en áreas rurales de difícil acceso y con muchas deficiencias en educación, alimentación y salud. El subregistro en la data epidemiológica limita la toma de decisiones conducentes a combatir y prevenir las enfermedades que afectan, con mayor frecuencia, a esta población. Se planteó evaluar la prevalencia de las principales afecciones que aquejan a la población criolla e indígena de la región de Maniapure, Municipio Cedeño, estado Bolívar - Venezuela. Métodos: Se realizó un estudio descriptivo de corte transversal durante la jornada de atención médica en la población de Maniapure. Se recolectaron datos sociodemográficos (edad, sexo y raza), motivos de consulta, hallazgos al examen físico, impresiones diagnósticas y tratamientos indicados. Éstos fueron obtenidos, a través de la historia clínica y analizados, mediante frecuencias y porcentajes. Resultados: Se evaluó un total de 359 pacientes, de los cuales el 48.8% (n = 175) fueron adultos y el 51.2% (n = 184) niños. Además, el 51.2% (n = 184) correspondió a personas de raza E'ñepá. La especialidad más requerida durante las jornadas fue medicina interna, con un 36.7% (n = 140) de las consultas. El principal motivo de consulta de los pacientes indígenas fue fiebre con el 62.5% (n = 115) de los casos; y de los pacientes criollos, fue la tos con un 28% (n = 49) de los casos. Asimismo, el hallazgo al examen físico más común fueron las lesiones de piel. Entre las impresiones diagnósticas, el 29.2% (n = 137) de los casos correspondieron al síndrome febril agudo, el 36.9% (n = 173) a enfermedades infecciosas, el 11.5% (n=45) a aquellos aparentemente sanos y el 22.4% (n = 114) a otras patologías. Conclusiones: En la población estudiada, se reporta como especialidad más requerida la de Medicina Interna. Además, las principales impresiones diagnósticas fueron el síndrome febril agudo, las parasitosis intestinales y la sospecha de malaria. Estos datos que deben ser considerados en una futura intervención.


Introduction: The indigenous population of the State of Bolivar lives in rural areas with difficult access and with many deficiencies in education, food and health. Under-recording in epidemiological data limits decision-making to combat and prevent the diseases that most frequently affect this population. It was proposed to evaluate the prevalence of the main diseases affecting the Creole and indigenous populations of the region of Maniapure, Municipality of Cedeño, State of Bolivar-Venezuela. Methods: A cross-sectional descriptive study was carried out during the day of medical attention in the population of Maniapure. Sociodemographic data were collected (age, sex, race), reasons for consultation, findings on physical examination, diagnostic impressions and indicated treatments. These were obtained through the clinical history and analyzed through frequencies and percentages. Results: A total of 359 patients were evaluated, of whom 48.8% (n=175) were adults and 51.2% (n=184) children. The 51.2% (n=184) corresponded to E'ñepá people. The specialty most required during the conference was internal medicine, with 36.7% (n=140) of the consultations. The main reason for consultation of indigenous patients was fever with 62.5% (n=115) of the cases; and of Creole patients, cough with 28% (n=49) of the cases. The most common physical examination was skin lesions. Among the diagnostic impressions, 29.2% (n=137) of the cases corresponded to acute febrile syndrome, 36.9% (n=173) to infectious diseases, 11.5% (n=45) to those apparently healthy and 22.4% (n=114) to other pathologies. Conclusions: In the population studied, Internal Medicine is reported as the most required specialty. In addition, the main diagnostic impressions were acute febrile syndrome, intestinal parasitosis and suspected malaria. Data that should be considered in a future intervention.


Asunto(s)
Humanos , Masculino , Femenino , Morbilidad , Atención Médica , Epidemiología Descriptiva , Estudios Transversales , Estudios Observacionales como Asunto
14.
Colloids Surf B Biointerfaces ; 169: 486-493, 2018 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-29860013

RESUMEN

We study how cell motility affects the stains left by the evaporation of droplets of a biofluid suspension containing mouse spermatozoa. The suspension, which contains also a high concentration of salts usually needed by motile cells, forms, upon drying, a crystallized pattern. We examine the structural characteristics of such patterns by optical microscopy. The analysis reveals that cell motility affects the formation of elongated crystals with lateral tips, as well as the creation of interlocked aggregates. We prove that a lacunarity algorithm based on polar symmetry, distinguishes among deposits generated by motile and non-motile cells with an accuracy greater than 95%.


Asunto(s)
Colorantes/química , Espermatozoides/citología , Algoritmos , Animales , Colorantes/síntesis química , Epidídimo/citología , Masculino , Ratones , Tamaño de la Partícula , Propiedades de Superficie
15.
J Helminthol ; 92(6): 752-759, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-29151395

RESUMEN

A new genus and species of nematode, Tziminema unachi n. gen., n. sp. is described from the caecum and colon of Baird's tapir Tapirus bairdii (Gill, 1865), found dead in the Reserva de la Biósfera El Triunfo, Chiapas State, in the Neotropical realm of Mexico. Tziminema n. gen. differs from the other nine genera included in the Strongylinae by two main characteristics: having 7-9 posteriorly directed tooth-like structures at the anterior end of the buccal capsule, and the external surface of the buccal capsule being heavily striated. Phylogenetic analyses of the DNA sequences of the mitochondrial cytochrome c oxidase and nuclear DNA, including a partial sequence of the internal transcribed spacer 1, 5.8S and a partial sequence of the internal transcribed spacer 2 of the new taxon, confirmed its inclusion in Strongylinae and its rank as a new genus.


Asunto(s)
Perisodáctilos/parasitología , Strongyloidea/clasificación , Strongyloidea/aislamiento & purificación , Animales , Ciego/parasitología , Análisis por Conglomerados , Colon/parasitología , ADN de Helmintos/química , ADN de Helmintos/genética , ADN Ribosómico/química , ADN Ribosómico/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Complejo IV de Transporte de Electrones/genética , México , Microscopía , Microscopía Electrónica de Rastreo , Filogenia , ARN Ribosómico 5.8S/genética , Análisis de Secuencia de ADN , Strongyloidea/anatomía & histología , Strongyloidea/genética
16.
Epidemiol Infect ; 145(15): 3125-3130, 2017 11.
Artículo en Inglés | MEDLINE | ID: mdl-28956522

RESUMEN

Bovine leukaemia virus (BLV) is the causative agent of enzootic bovine leucosis, which has been reported worldwide. BLV has been found recently in human tissue and it could have a significant impact on human health. A possible hypothesis regarding viral entry to humans is through the consumption of infected foodstuffs. This study was aimed at detecting the presence of BLV DNA in raw beef and fresh milk for human consumption. Nested PCR directed at the BLV gag gene (272 bp) was used as a diagnostic test. PCR products were confirmed by Sanger sequencing. Forty-nine per cent of the samples proved positive for the presence of proviral DNA. This is the first study highlighting the presence of the BLV gag gene in meat products for human consumption and confirms the presence of the viral DNA in raw milk, as in previous reports. The presence of viral DNA in food products could suggest that viral particles may also be found. Further studies are needed to confirm the presence of infected viral particles, even though the present findings could represent a first approach to BLV transmission to humans through foodstuff consumption.


Asunto(s)
ADN Viral/genética , Leucosis Bovina Enzoótica/transmisión , Virus de la Leucemia Bovina , Carne/virología , Leche/virología , Animales , Bovinos , Humanos , Virus de la Leucemia Bovina/genética , Reacción en Cadena de la Polimerasa Multiplex , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN
17.
J Parasitol ; 102(5): 553-555, 2016 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-27391275

RESUMEN

Invertebrate-derived ingested DNA (iDNA) is quickly proving to be a valuable, non-invasive tool for monitoring vertebrate species of conservation concern. Using the DNA barcoding locus, we successfully identified both the blood-feeding leech Haementeria acuecueyetzin and its blood meal-the latter is shown to be derived from the Caribbean manatee, Trichechus manatus . DNA amplification was successful despite the fact that the specimen was fixed in Mezcal (a beverage distilled from agave). We report the first confirmed case of a leech feeding on a manatee, the first record of H. acuecueyetzin for the State of Chiapas and, to our knowledge, the first case of successful DNA amplification of a biological sample fixed in Mezcal other than the caterpillar "worms" more commonly found in that beverage.


Asunto(s)
Agave/parasitología , Código de Barras del ADN Taxonómico/veterinaria , Jugos de Frutas y Vegetales/parasitología , Sanguijuelas/clasificación , Trichechus/parasitología , Animales , Femenino , Fijadores , Sanguijuelas/genética , México , Filogenia , Trichechus/sangre , Indias Occidentales
18.
Rev. chil. urol ; 81(1): 35-39, 2016.
Artículo en Inglés | LILACS | ID: biblio-906320

RESUMEN

Objectives: To evaluate an alternative approach to tubeless surgery that allows a second per- cutaneous procedure using the same nephrostomy tract. Methods: Twenty patients underwent percutaneous nephrolithotomy from September 2012 to May 2013 at our institution. Inclusion criteria were: absence of urinary infection, single puncture and operative time less than 2 h. Following the procedure the initially placed ureteral catheter was exteriorized through the working sheath by tying a non-absorbable suture to its end. On postoperative day 1 all patients were studied with non-enhanced CT or X-ray film. If the patient was rendered stone free, the stent was removed along with the urethro-vesical catheter. If a residual stone was present, we recovered the ureteral catheter and used the same nephros- tomy tract for a second endoscopic procedure. Patients were assessed for pain, postoperative complications, length of stay, stone free rate, hematocrit and creatinine variations. Results: Thirteen patients met the inclusion criteria. No major complications related to the stent placement and its exteriorization were seen. Two patients required a second percutaneous procedure successfully achieved recovering the ureteral catheter through the nephrostomy tract. Conclusion: We present a safe and simple modification of tubeless percutaneous nephrolitho- tomy, with its well-known clinical benefits but maintaining a safe path for an eventual second look procedure if necessary.(AU)


Objetivos: Evaluar una sencilla modificación de la nefrolitectomía percutánea tubeless que permita un segundo procedimiento endoscópico utilizando el mismo trayecto percutáneo. Métodos: Veinte pacientes fueron sometidos a nefrolitectomía percutánea en decúbito supino modificado entre septiembre de 2012 y mayo de 2013 en nuestro centro. Los criterios de inclusión para el estudio fueron: ausencia de infección urinaria, punción única y tiempo opera- torio menor de 2 h. Al finalizar el procedimiento se instaló una rienda de sutura en el extremo del catéter ureteral, quedando exteriorizada a través del trayecto de nefrostomía para su recuperación en caso de necesidad. Se realizó tomografía computarizada o radiografía simple a todos los pacientes el primer día postoperatorio. Si el paciente estaba «libre de cálculos¼, el catéter ureteral fue retirado junto con la sonda Foley. En caso de litiasis residual se realizó un segundo procedimiento percutáneo, utilizando el mismo trayecto inicial, exteriorizando el catéter ureteral a través del trayecto de la nefrostomía. En todos los pacientes se objetivaron las complicaciones postoperatorias, la estadía hospitalaria y la presencia de litiasis residual, además de la caída del hematocrito y la creatinina. Resultados: Trece pacientes cumplieron los criterios de inclusión. No hubo complicaciones may- ores relacionadas con la colocación del catéter ureteral y su exteriorización. Dos pacientes requirieron una segunda intervención percutánea, lograda con éxito mediante el uso del catéter ureteral reexteriorizado. Conclusión: Se presenta una modificación segura y sencilla de la nefrolitectomía percutánea tubeless sin renunciar a la posibilidad de recuperar el trayecto de nefrostomía original.(AU)


Asunto(s)
Humanos , Nefrostomía Percutánea , Nefrolitotomía Percutánea
19.
Environ Monit Assess ; 187(1): 4158, 2015 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-25523271

RESUMEN

The mining district of El Triunfo (ET-MD) has an estimated 800,000 t of mine wastes scattered in the environment, contaminating the sediment with potentially toxic elements such as As, Cd, Pb, and Zn. In order to estimate the toxicity of the sediment to the adjacent biota, the aims of our study are to calculate the mortality and inhibition through bioassays, using sediment, and test organisms such as Daphnia magna and Selenastrum capricornutum (Pseudokirchneriella subcapitata), respectively. The D. magna mortality was 31 ± 12% and the S. capricornutum growth inhibition was 53 ± 24%. The contamination of the sediment determines the high mortality of D. magna and the high inhibition of S. capricornutum in the system, indicating risk for the biota in the contaminated system.


Asunto(s)
Monitoreo del Ambiente , Sedimentos Geológicos/análisis , Minería , Contaminantes del Suelo/toxicidad , Animales , Bioensayo , Chlorophyta , Daphnia , Sedimentos Geológicos/química , Oro , Contaminantes del Suelo/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad
20.
Odontol. pediatr. (Lima) ; 13(1): 17-24, ene.-jun.2014. ilus, graf
Artículo en Español | LILACS, LIPECS | ID: lil-781157

RESUMEN

El Agregado trióxido agregado (MTA) es un cemento dental que tiene como base el cemento portland de construcción. Posee buenas propiedades estructurales y regeneradoras. El objetivo de este estudio fue comprobar si el cemento Yura Portland posee las mismas características estructurales, químicas y regeneradoras que el MTA...


The mineral trioxide aggregate is a dental cement which is based on portland cement for construction. It has good structural and regenerative properties. The aim of this study was to test if the Yura Portland cement has the same strudural characteristics, chemical and regenerating the MTA...


Asunto(s)
Humanos , Cementos Dentales
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA