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Bioinformatics ; 34(17): 3058-3060, 2018 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-29659702

RESUMEN

Summary: MALDI-TOF MS is a rapid, sensitive and economic tool for bacterial identification. Highly abundant bacterial proteins are detected by this technique, including ribosomal proteins (r-protein), and the generated mass spectra are compared with a MALDI-TOF MS spectra database. Currently, it allows mainly the classification of clinical bacteria due to the limited number of environmental bacteria included in the spectra database. We present a wide-ranging bacterium classifier tool, called Ribopeaks, which was created based on r-protein data from the Genbank. The Ribopeaks database has more than 28 500 bacterial taxonomic records. It compares the incoming m/z data from MALDI-TOF MS analysis with models stored in the Ribopeaks database created by machine learning and then taxonomically classifies the bacteria. Availability and implementation: The software is available at http://www.ribopeaks.com. Supplementary information: Supplementary data are available at Bioinformatics online.


Asunto(s)
Bacterias/clasificación , Proteínas Bacterianas/análisis , Proteínas Ribosómicas/análisis , Proteínas Bacterianas/química , Proteínas Ribosómicas/química , Programas Informáticos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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