RESUMEN
The effects of the allelochemical benzoxazolin-2-(3H)-one (BOA) were evaluated on growth, lignin content and its monomers p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) in roots, stems and leaves of soybean. BOA decreased the lengths and fresh weights of roots and stems, and the fresh weights and areas of leaves. Reductions in the growth were accompanied by enhanced lignin content in all tissues. In roots, the allelochemical increased the content of H, G and S monomers as well as the overall amount of lignin (referred to as the sum of H+G+S), but did not alter the S/G ratio. In stems and leaves, BOA increased the H, G, S and H+G+S contents while decreasing the S/G ratio. In brief, BOA-induced inhibition of soybean may be due to excessive production of monomers that increase the degree of polymerization of lignin, limit cell expansion, solidify the cell wall and restrict plant growth.
Asunto(s)
Benzoxazoles/farmacología , Glycine max/crecimiento & desarrollo , Glycine max/metabolismo , Lignina/metabolismo , Biomasa , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Raíces de Plantas/anatomía & histología , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Tallos de la Planta/efectos de los fármacos , Tallos de la Planta/metabolismo , Glycine max/efectos de los fármacosRESUMEN
Cinnamic acid and its hydroxylated derivatives (p-coumaric, caffeic, ferulic and sinapic acids) are known allelochemicals that affect the seed germination and root growth of many plant species. Recent studies have indicated that the reduction of root growth by these allelochemicals is associated with premature cell wall lignification. We hypothesized that an influx of these compounds into the phenylpropanoid pathway increases the lignin monomer content and reduces the root growth. To confirm this hypothesis, we evaluated the effects of cinnamic, p-coumaric, caffeic, ferulic and sinapic acids on soybean root growth, lignin and the composition of p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S) monomers. To this end, three-day-old seedlings were cultivated in nutrient solution with or without allelochemical (or selective enzymatic inhibitors of the phenylpropanoid pathway) in a growth chamber for 24 h. In general, the results showed that 1) cinnamic, p-coumaric, caffeic and ferulic acids reduced root growth and increased lignin content; 2) cinnamic and p-coumaric acids increased p-hydroxyphenyl (H) monomer content, whereas p-coumaric, caffeic and ferulic acids increased guaiacyl (G) content, and sinapic acid increased sinapyl (S) content; 3) when applied in conjunction with piperonylic acid (PIP, an inhibitor of the cinnamate 4-hydroxylase, C4H), cinnamic acid reduced H, G and S contents; and 4) when applied in conjunction with 3,4-(methylenedioxy)cinnamic acid (MDCA, an inhibitor of the 4-coumarate:CoA ligase, 4CL), p-coumaric acid reduced H, G and S contents, whereas caffeic, ferulic and sinapic acids reduced G and S contents. These results confirm our hypothesis that exogenously applied allelochemicals are channeled into the phenylpropanoid pathway causing excessive production of lignin and its main monomers. By consequence, an enhanced stiffening of the cell wall restricts soybean root growth.
Asunto(s)
Pared Celular/metabolismo , Cinamatos , Glycine max/crecimiento & desarrollo , Lignina/biosíntesis , Raíces de Plantas/crecimiento & desarrollo , Cinamatos/metabolismo , Cinamatos/farmacología , Raíces de Plantas/citología , Glycine max/citologíaRESUMEN
Cinnamic acid is a known allelochemical that affects seed germination and plant root growth and therefore influences several metabolic processes. In the present work, we evaluated its effects on growth, indole-3-acetic acid (IAA) oxidase and cinnamate 4-hydroxylase (C4H) activities and lignin monomer composition in soybean (Glycine max) roots. The results revealed that exogenously applied cinnamic acid inhibited root growth and increased IAA oxidase and C4H activities. The allelochemical increased the total lignin content, thus altering the sum and ratios of the p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) lignin monomers. When applied alone or with cinnamic acid, piperonylic acid (PIP, a quasi-irreversible inhibitor of C4H) reduced C4H activity, lignin and the H, G, S monomer content compared to the cinnamic acid treatment. Taken together, these results indicate that exogenously applied cinnamic acid can be channeled into the phenylpropanoid pathway via the C4H reaction, resulting in an increase in H lignin. In conjunction with enhanced IAA oxidase activity, these metabolic responses lead to the stiffening of the cell wall and are followed by a reduction in soybean root growth.
Asunto(s)
Cinamatos/farmacología , Glycine max/crecimiento & desarrollo , Glycine max/metabolismo , Lignina/biosíntesis , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Benzoatos/farmacología , Biomasa , Lignina/química , Peroxidasas/metabolismo , Raíces de Plantas/anatomía & histología , Raíces de Plantas/enzimología , Plantones/efectos de los fármacos , Plantones/metabolismo , Glycine max/efectos de los fármacos , Transcinamato 4-Monooxigenasa/metabolismoRESUMEN
Coffee plants were subjected to heat stress (37 °C) and compared with control plants (24 °C). Cell wall polysaccharides were extracted using water (W), EDTA (E) and 4M NaOH (H30 and H70). In addition, monolignols were analyzed, and the leaves were observed by microscopy. Plants under heat stress accumulated higher contents of arabinose and galactose in fraction W. Xylose contents were observed to decrease in H30 fractions after the heat stress, whereas galactose and uronic acid increased. H70 fractions from plants exposed to heat stress showed increased xylose contents, whereas the contents of arabinose and glucose decreased. Differences in the molar-mass profiles of polysaccharides were also observed. The primary monolignol contents increased after the heat stress. Structural alterations in palisade cells and ultrastructural damage in chloroplasts were also observed. Our results demonstrate that the chemical profile of coffee cell-wall polymers and structural cell anatomy change under heat stress.
Asunto(s)
Pared Celular/química , Coffea/química , Hojas de la Planta/anatomía & histología , Estrés Fisiológico , Arabinosa/química , Cromatografía en Gel/métodos , Coffea/anatomía & histología , Galactanos/química , Calor , Lignina/química , Pectinas/química , Células Vegetales/química , Polisacáridos/química , Ácidos Urónicos/química , Agua/químicaRESUMEN
The allelopathic effect of caffeic acid was tested on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, hydrogen peroxide (H(2)O(2)) accumulation, lignin content and monomeric composition of soybean (Glycine max) roots. We found that exogenously applied caffeic acid inhibited root growth, decreased the PAL activity and H(2)O(2) content and increased the soluble and cell wall-bound POD activities. The p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) monomers and total lignin (H+G+S) increased in the caffeic acid-exposed roots. When applied in conjunction with piperonylic acid (PIP, an inhibitor of the cinnamate 4-hydroxylase, C4H), caffeic acid equalized the inhibitory effect of PIP, whereas the application of methylene dioxocinnamic acid (MDCA, an inhibitor of the 4-coumarate:CoA ligase, 4CL) plus caffeic acid decreased lignin production. These results indicate that exogenously applied caffeic acid can be channeled into the phenylpropanoid pathway via the 4CL reaction, resulting in an increase of lignin monomers that solidify the cell wall and inhibit root growth.
Asunto(s)
Antioxidantes/farmacología , Ácidos Cafeicos/farmacología , Glycine max/efectos de los fármacos , Lignina/metabolismo , Benzoatos/farmacología , Pared Celular/enzimología , Pared Celular/metabolismo , Cinamatos/farmacología , Coenzima A Ligasas/antagonistas & inhibidores , Coenzima A Ligasas/metabolismo , Ácidos Cumáricos/farmacología , Inhibidores Enzimáticos/farmacología , Peróxido de Hidrógeno/análisis , Peróxido de Hidrógeno/metabolismo , Lignina/análisis , Peroxidasa/efectos de los fármacos , Peroxidasa/metabolismo , Fenilanina Amoníaco-Liasa/efectos de los fármacos , Fenilanina Amoníaco-Liasa/metabolismo , Proteínas de Plantas/efectos de los fármacos , Proteínas de Plantas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/enzimología , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Plantones/efectos de los fármacos , Plantones/enzimología , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Glycine max/enzimología , Glycine max/crecimiento & desarrollo , Glycine max/metabolismo , Transcinamato 4-Monooxigenasa/antagonistas & inhibidores , Transcinamato 4-Monooxigenasa/metabolismoRESUMEN
The effects of cadmium (Cd), a well-known environmental pollutant with high toxicity to plants, were tested on root growth, cell viability, phenylalanine ammonia-lyase (PAL) soluble plus cell wall-bound peroxidase (POD) activities, hydrogen peroxide (H(2)O(2)) levels, and the content and monomeric composition of lignin in soybean (Glycine max) roots. Three-day-old seedlings were cultivated in half-strength Hoagland's solution (pH 6.0), with or without 25-100 µM CdCl(2) in a growth chamber (25°C, 12/12-h light/dark photoperiod, irradiance of 280 µmolm(-2)s(-1)) for 24h. In general, root length and the fresh and dry weights decreased followed by loss of cell viability after Cd treatment. PAL activity, soluble and cell wall-bound POD activities, and H(2)O(2) and lignin contents increased significantly after Cd exposure. The lignin monomeric composition of Cd-exposed roots revealed a significant increase of p-hydroxyphenyl (H) and syringyl (S) units. These results suggest that the effects caused by Cd may be due to excessive production of monolignols forming lignin, which solidifies the cell wall and restricts root growth.
Asunto(s)
Cloruro de Cadmio/toxicidad , Contaminantes Ambientales/toxicidad , Glycine max/efectos de los fármacos , Lignina/metabolismo , Fotoperiodo , Supervivencia Celular/efectos de los fármacos , Pared Celular/efectos de los fármacos , Pared Celular/metabolismo , Contaminantes Ambientales/química , Peróxido de Hidrógeno/metabolismo , Peroxidasas/metabolismo , Fenilanina Amoníaco-Liasa/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Glycine max/crecimiento & desarrollo , Glycine max/metabolismo , Factores de TiempoRESUMEN
The flavanone naringenin, an intermediate in flavonoid biosynthesis, was tested for its effect on root growth, phenylalanine ammonia-lyase (PAL) and peroxidase (POD) activities, as well as phenolic compounds and lignin contents in soybean (Glycine max L. Merrill) seedlings. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), with or without 0.1 to 0.4 mM naringenin in a growth chamber (25°C, 12-h photoperiod, irradiance of 280 µmol m-2 s-1) for 24 h. Inhibitory effects on root growth (length, weight, cell viability), PAL and soluble POD activities were detected after naringenin treatments. These effects were associated with stimulatory activity of the cell wall-bound POD followed by an increase in the lignin contents, suggesting that naringenin-induced inhibition in soybean roots could be due to the lignification process.
Os efeitos de naringenina, um intermediário da biossíntese de flavonóides, foram avaliados sobre o crescimento das raízes, as atividades da fenilalanina amônia liase (PAL) e peroxidases, bem como sobre os teores de compostos fenólicos e de lignina em plântulas de soja (Glycine max L. Merrill). Plântulas de três dias foram cultivadas em solução nutritiva de Hoagland, meia-força (pH 6,0), contendo ou não, naringenina 0,1 a 0,4 mM, em uma câmara de germinação (25°C, fotoperíodo de 12 h, 280 µmol m-2 s-1) durante 24 h. Efeitos inibitórios no crescimento das raízes (comprimento, massa e viabilidade celular) e nas atividades da PAL e POD solúvel foram constatados após os tratamentos com naringenina. Estes efeitos foram associados com atividade estimulatória da POD ligada à parede celular, seguido por aumento nos teores de lignina, sugerindo que a inibição do crescimento das raízes pode ser devido ao processo de lignificação.
RESUMEN
L-3,4-dihydroxyphenylalanine (L: -DOPA), an allelochemical exuded from the roots of velvet bean [Mucuna pruriens (L.) DC. var. utilis], presents a highly inhibitory action to plant growth. The effects of L-DOPA on phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and peroxidase (POD, EC 1.11.1.7) activities, and phenolic compound and lignin content in soybean [Glycine max (L.) Merr.] roots were investigated to determine the possible phytotoxic mechanism. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), without or with 0.1 to 1.0 mM L-DOPA in a growth chamber (25 degrees C, 12-hr light to 12-hr darkness photoperiod, irradiance of 280 micromol m-2 s-1) for 24 hr. In general, the length, fresh weight, and dry weight of the roots decreased, whereas PAL and POD activities and phenolic compound and lignin content increased after L-DOPA treatments. Results showed the susceptibility of soybean to L-DOPA and reinforce the role of this nonprotein amino acid as a strong allelochemical. The present findings also suggest that L-DOPA-induced inhibition in soybean roots may be because of a cell wall stiffening process related to the formation of cross-linking between cell wall polymers linked to lignin production.
Asunto(s)
Glycine max/efectos de los fármacos , Levodopa/farmacología , Lignina/metabolismo , Reguladores del Crecimiento de las Plantas/farmacología , Raíces de Plantas/efectos de los fármacos , Mucuna/química , Peroxidasa/efectos de los fármacos , Peroxidasa/metabolismo , Fenilanina Amoníaco-Liasa/efectos de los fármacos , Fenilanina Amoníaco-Liasa/metabolismo , Raíces de Plantas/metabolismo , Glycine max/crecimiento & desarrollo , Glycine max/metabolismoRESUMEN
This study proposes a simple, quick and reliable method for determining the cinnamyl alcohol dehydrogenase (CAD; EC 1.1.1.195) activity in soybean (Glycine max L. Merr.) roots using reversed-phase high performance liquid chromatography (RP-HPLC). The method includes a single extraction of the tissue and conduction of the enzymatic reaction at 30 degrees C with cinnamaldehydes (coniferyl or sinapyl), substrates of CAD. Disappearance of the substrates in the reaction mixture is monitored at 340 nm (for coniferaldehyde) or 345 nm (for sinapaldehyde) by isocratic elution with methanol/acetic acid through a GLC-ODS (M) column. This HPLC technique furnishes a rapid and reliable measure of cinnamaldehyde substrates, and may be used as an alternative tool to analyze CAD activity in enzyme preparation without previous purification.