RESUMEN
Embryo biopsy for fetal sexing has clinical application, but few reports are available of its use within an active embryo transfer program. We evaluated results on biopsy of 459 embryos over one breeding season. There were no significant differences in pregnancy rate between biopsied and non-biopsied embryos (72% vs 73%) or for biopsied embryos recovered at the centre (73%) compared with those shipped overnight (72%). However, the pregnancy rate decreased significantly in shipped embryos biopsied ≥20h after collection. Overall, 86% of biopsies provided a sex diagnosis. The likelihood of a positive genomic (g) DNA result was significantly higher for biopsies from large blastocysts (96%) than from smaller embryos (70-85%). In total, 38% of biopsies were positive for Y chromosome DNA (Y-DNA) and were diagnosed as male. Subsequently, 95% of Y-DNA-positive embryos were confirmed as male and 78% of Y-DNA-negative embryos were confirmed as female. The accuracy of prediction of female (Y-DNA negative) was significantly higher when the biopsy sample was probed for Y-DNA only compared with probing for both gDNA and Y-DNA. We estimate that by transferring only Y-DNA-negative embryos, 3% of potential female pregnancies may have been lost, and production of male pregnancies was reduced by 72%.
Asunto(s)
Blastocisto/patología , Embrión de Mamíferos/patología , Caballos/embriología , Reacción en Cadena de la Polimerasa , Diagnóstico Preimplantación , Análisis para Determinación del Sexo , Animales , Argentina , Biopsia , Cruzamiento/economía , Cruzamiento/métodos , Comercio , Transferencia de Embrión/economía , Transferencia de Embrión/métodos , Transferencia de Embrión/veterinaria , Femenino , Masculino , Reacción en Cadena de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa/veterinaria , Embarazo , Diagnóstico Preimplantación/métodos , Diagnóstico Preimplantación/veterinaria , Análisis para Determinación del Sexo/métodos , Análisis para Determinación del Sexo/veterinaria , Medicina Veterinaria Deportiva/economía , Medicina Veterinaria Deportiva/métodos , Medicina Veterinaria Deportiva/organización & administraciónAsunto(s)
Hemangioma Cavernoso/patología , Hueso Occipital/patología , Hueso Parietal/patología , Neoplasias Craneales/patología , Femenino , Hemangioma Cavernoso/complicaciones , Hemangioma Cavernoso/epidemiología , Humanos , Persona de Mediana Edad , Osteólisis/etiología , Estudios Retrospectivos , Neoplasias Craneales/complicaciones , Neoplasias Craneales/epidemiologíaRESUMEN
Dr. J. Emilio Fernández, important figure of Rosarine Odontology, has been one of the pioneers in the growing of its societary movement and principal promotor in the foundation of the Odontologic Circle Rosario Museum. In this article the origins of the Museum in his charge are described from its foundation up to of present, depicting the principal pieces of its rich collection.
Asunto(s)
Odontología , Museos/historia , Argentina , Historia del Siglo XXRESUMEN
Dr. J. Emilio Fernández, important figure of Rosarine Odontology, has been one of the pioneers in the growing of its societary movement and principal promotor in the foundation of the Odontologic Circle Rosario Museum. In this article the origins of the Museum in his charge are described from its foundation up to of present, depicting the principal pieces of its rich collection.
RESUMEN
Dr. J. Emilio Fernández, important figure of Rosarine Odontology, has been one of the pioneers in the growing of its societary movement and principal promotor in the foundation of the Odontologic Circle Rosario Museum. In this article the origins of the Museum in his charge are described from its foundation up to of present, depicting the principal pieces of its rich collection.
RESUMEN
A total of 150 oregano samples from 6 different trade marks were studied. These samples were obtained at retail shops in Buenos Aires city and they were analyzed in order to determine the presence of the following microorganisms: 1) mesophilic aerobic plate count; 2) molds; 3) coliforms and fecal coliforms; 4) enterococci; 5) aerobes sporeforming: a) mesophilic aerobes; a1) total aerobes; a2) mesophilic anaerobes; b) thermophilic; b1) sulfide spoilage sporeformers; b2) total aerobes; b3) thermo-philic Flat sour; 6) pathogens; 6a) Clostridium perfringens 6b) Bacillus cereus and 6c) Staphylococcus aureus. We can conclude from these results that there is no apparent risk for public health. Nevertheless the microbiologic evaluation of the spices becomes important when they are added to packed foodstuff which has not received a minimum thermic treatment.
Asunto(s)
Condimentos/análisis , Microbiología de Alimentos , Magnoliopsida/análisis , Bacterias/aislamiento & purificación , Conservación de Alimentos , Hongos/aislamiento & purificaciónRESUMEN
Se estudiaron 150 muestras de orégano de seis marcas distintas adquiridas en comercios minoristas de la Capital Federal. En ellas se hicieron los siguientes recuentos microbianos: aerobios mesófilos; hongos; coliformes totales y fecales; esporoformadores: mesófilos (aerobios totales y anaerobios tipo putrefactivo) y termófilos (anaerobios productores de sulfhídrico, aerobios totales, responsables de la fermentación simple o agriado); Clostidium perfringens; Bacillkus cereus; Staphylococcus aureus coagulasa positivo. De los resultados obtenidos es posible concluir que no existe riesgo aparente para la salud pública. Sin embargo la evaluación microbiológica de la especia adquiere importancia si ésta se adiciona a alimentos envasados que no han recibido un mínimo tratamiento térmico
Asunto(s)
Condimentos/análisis , Microbiología de Alimentos , Plantas Medicinales/análisis , Bacterias/aislamiento & purificación , Conservación de Alimentos , Hongos/aislamiento & purificaciónRESUMEN
A total of 150 oregano samples from 6 different trade marks were studied. These samples were obtained at retail shops in Buenos Aires city and they were analyzed in order to determine the presence of the following microorganisms: 1) mesophilic aerobic plate count; 2) molds; 3) coliforms and fecal coliforms; 4) enterococci; 5) aerobes sporeforming: a) mesophilic aerobes; a1) total aerobes; a2) mesophilic anaerobes; b) thermophilic; b1) sulfide spoilage sporeformers; b2) total aerobes; b3) thermo-philic Flat sour; 6) pathogens; 6a) Clostridium perfringens 6b) Bacillus cereus and 6c) Staphylococcus aureus. We can conclude from these results that there is no apparent risk for public health. Nevertheless the microbiologic evaluation of the spices becomes important when they are added to packed foodstuff which has not received a minimum thermic treatment.
RESUMEN
Se estudiaron 150 muestras de orégano de seis marcas distintas adquiridas en comercios minoristas de la Capital Federal. En ellas se hicieron los siguientes recuentos microbianos: aerobios mesófilos; hongos; coliformes totales y fecales; esporoformadores: mesófilos (aerobios totales y anaerobios tipo putrefactivo) y termófilos (anaerobios productores de sulfhídrico, aerobios totales, responsables de la fermentación simple o agriado); Clostidium perfringens; Bacillkus cereus; Staphylococcus aureus coagulasa positivo. De los resultados obtenidos es posible concluir que no existe riesgo aparente para la salud pública. Sin embargo la evaluación microbiológica de la especia adquiere importancia si ésta se adiciona a alimentos envasados que no han recibido un mínimo tratamiento térmico (AU)