RESUMEN
We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1%, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100% of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.
Asunto(s)
Recuento de Colonia Microbiana/veterinaria , Infecciones por Enterobacteriaceae/veterinaria , Enterobacteriaceae/aislamiento & purificación , Escherichia coli/aislamiento & purificación , Enfermedades de los Peces/microbiología , Tilapia/microbiología , Animales , Acuicultura , Recuento de Colonia Microbiana/métodos , Infecciones por Enterobacteriaceae/microbiología , Extremidades/microbiología , Agua Dulce , Branquias/microbiología , Piel/microbiologíaRESUMEN
We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100 of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.
Asunto(s)
Animales , Recuento de Colonia Microbiana/veterinaria , Enfermedades de los Peces/microbiología , Enterobacteriaceae , Escherichia coli , Infecciones por Enterobacteriaceae/veterinaria , Tilapia , Acuicultura , Recuento de Colonia Microbiana/métodos , Extremidades , Agua Dulce , Branquias , Infecciones por Enterobacteriaceae/microbiología , PielRESUMEN
We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapias external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100 of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.(AU)
Asunto(s)
Estudio Comparativo , Animales , Recuento de Colonia Microbiana/veterinaria , Enterobacteriaceae/aislamiento & purificación , Infecciones por Enterobacteriaceae/veterinaria , Escherichia coli/aislamiento & purificación , Enfermedades de los Peces/microbiología , Tilapia/microbiología , Acuicultura , Recuento de Colonia Microbiana/métodos , Infecciones por Enterobacteriaceae/microbiología , Extremidades/microbiología , Agua Dulce , Branquias/microbiología , Piel/microbiologíaRESUMEN
We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapias external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1
, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100
of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.