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Biosens Bioelectron ; 50: 437-40, 2013 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-23896524

RESUMEN

A surface confined redox group contributes to an interfacial charging (quantifiable by redox capacitance) that can be sensitively probed by impedance derived capacitance spectroscopy. In generating mixed molecular films comprising such redox groups, together with specific recognition elements (here antibodies), this charging signal is able to sensitively transduce the recognition and binding of specific analytes. This novel transduction method, exemplified here with C-reactive protein, an important biomarker of cardiac status and general trauma, is equally applicable to any suitably prepared interfacial combination of redox reporter and receptor. The assays are label free, ultrasensitive, highly specific and accompanied by a good linear range.


Asunto(s)
Técnicas Biosensibles/métodos , Proteína C-Reactiva/análisis , Anticuerpos Inmovilizados/química , Capacidad Eléctrica , Inmunoensayo/métodos , Límite de Detección , Modelos Moleculares , Oxidación-Reducción , Análisis Espectral
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