RESUMEN
BACKGROUND: This study describes the bone formation stimulated by the application of a type of chalcone to critical-size defects in rat calvarial bone. MATERIAL AND METHODS: Sixty female Wistar rats were divided into 6 groups of 10 animals per group: control (no treatment), vehicle (vaseline) and the chalcone (1-phenyl-3-(4-chlorophenyl)-2-propen-1-one) suspended in vaseline at 10%. A critical-size defect of 5 mm was prepared using a trephine in the calvarial bone, after which the treatment was applied, in a single dose, according to the experimental group. The samples were evaluated macroscopically using ImageJ software, and histologically 30 and 45 days after surgery. RESULTS: At 30 days after surgery, there was significant bone formation (p < 0.05) in the groups treated with chalcone, compared with the other groups. Many active osteoblasts were observed adjacent to the borders of the newly formed bone tissue. 45 days after surgery in the chalcone group, the surgical defects showed complete bone closure. CONCLUSION: The results of this study suggest that chalcone has significant potential to induce the formation of new bone.
Asunto(s)
Enfermedades Óseas/tratamiento farmacológico , Chalcona/uso terapéutico , Chalconas/uso terapéutico , Osteogénesis/efectos de los fármacos , Cráneo/efectos de los fármacos , Acetofenonas/química , Animales , Benzaldehídos/química , Matriz Ósea/efectos de los fármacos , Matriz Ósea/patología , Calcificación Fisiológica/efectos de los fármacos , Chalcona/síntesis química , Tejido Conectivo/efectos de los fármacos , Tejido Conectivo/patología , Femenino , Osteoblastos/efectos de los fármacos , Osteoblastos/patología , Osteocitos/efectos de los fármacos , Osteocitos/patología , Vaselina , Vehículos Farmacéuticos , Ratas , Ratas Wistar , Cráneo/patología , Hidróxido de Sodio/química , Factores de TiempoRESUMEN
Chalcones belong to a class of alpha,beta,-unsaturated aromatic ketones which occur abundantly in nature, especially in plants. They are promising and interesting compounds due to their vast applications in pharmaceuticals, agriculture and industry. Several studies have shown that these compounds exert important biological activities in different experimental models. The present work deals with the antinociceptive activity, evaluated against the writhing test, of three series of chalcone-like compounds obtained by the Claisen-Schmidt condensation, using different aldehydes and substituted acetophenones. The results reveal that the compounds synthesized show a significant antinociceptive effect compared with nonsteroidal drugs such as aspirin, paracetamol and diclofenac. They also show that the electronic demand of the substituents is the dominant factor of the biological activity.