RESUMEN
OBJECTIVE: To explore the changes and regulations in T-scores of hip and lumbar vertebrae in different aged people through retrospective data analyses of dual energy X-ray absorptiometry (DXA) and improve their reference values. METHODS: With the aid of DXA, the bone densities of hip (femoral neck) and lumbar (L1-L4) bones were measured in 3662 males and females over 45 years old. There were 9 female and 9 males groups based upon the stratifications of gender and 5-year age intervals (> 85-year-olds combined as one group). After ruling out the "single-part test" data, the T-score averages were calculated and their differences analyzed by t-test. RESULTS: In females, T-scores of hip and lumbar bone mineral density were basically the same before 50 years old. Significant differences existed between 51 and 75 years old females. The lumbar T-scores were lower than their hip counterparts. In some age groups, T-scores of lumbar vertebrae showed decreased bone mass while those of hip remained normal. However, T-scores of lumbar vertebrae might be diagnosed by osteoporosis but decreased bone mass of hip was absent in the other age groups. After 75 years, no obvious differences existed between hip and lumbar bone T-scores; in all male groups, hip T-scores were lower than lumbar counterparts. There were small changes in T-scores of lumbar vertebrae with aging while the hip ones decreased gradually; each group had a single measurement rate of skeletal site. In females, the minimal and maximal values of single site measurement rate were 12.50% and 63.98% versus 16.68% and 44.50% in males. CONCLUSION: Differences of lumbar and hip T-scores exist in some age groups. Thus some normal T-scores of one skeletal site may be abnormal at another site. While evaluating one particular site, one should consider these differences to avoid an incorrect diagnosis.
Asunto(s)
Densidad Ósea , Cadera/fisiología , Vértebras Lumbares/fisiología , Absorciometría de Fotón , Anciano , Anciano de 80 o más Años , Femenino , Cadera/diagnóstico por imagen , Humanos , Vértebras Lumbares/diagnóstico por imagen , Masculino , Persona de Mediana Edad , Valores de Referencia , Estudios RetrospectivosRESUMEN
OBJECTIVE: To address whether hepcidin functions in bone metabolism. METHODS: This study was carried out in the Laboratory of Radiation Medicine and Public Health of Soochow University, and the Laboratory of the Second Affiliated Hospital of Soochow University, Suzhou, China, from September 2009 to July 2010. The positive expression of ferroportin-1 (Fpn-1) was detected by reverse transcriptase-polymerase chain reaction. After the treatment with distilled water (control group) and hepcidin (25noml/L, 50noml/L, 100noml/L), the fluorescence intensity related to intracellular iron concentration of a human fetal osteoblast cell line (hFOB 1.19) was measured by a confocal laser scanning microscope. A 3-(4,5- dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide assay, and Von Kossa staining was performed to evaluate cell proliferation and mineralization in cultured hFOB 1.19 cells. RESULTS: This study revealed a high level expression of Fpn-1 in hFOB 1.19. On the basis of which, it was found that 25noml/L, 50noml/L, 100noml/L hepcidin could promote the fluorescence intensity related to intracellular iron concentration and mineralization in hFOB 1.19 in a dose-dependent manner (p<0.05), but hepcidin had no effect on FOB 1.19 proliferation (p>0.05). CONCLUSION: The hepcidin-ferroportin signal pathway may function in the osteoblast cell line of hFOB 1.19 cells. It is also suggested that cross-talk between iron and calcium homeostasis may play a role in bone metabolism in responding to hepcidin activation.