RESUMEN
Objective: To analyze the personal dose of radiation exposure of radiation workers in some medical institutions in Tianjin, and to provide reference for radiation protection work. Methods: Using cluster sampling method, 8718 radiation workers from some medical institutions (including tertiary, secondary and first-level hospitals) in Tianjin from 2014 to 2018 were selected as the subjects of investigation. Monitoring data were collected, analyzed and evaluated. Results: From 2014 to 2018, a total of 8718 persons were monitored, with 14 persons (0.2%) whose annual effective dose was higher than 5 mSv; 8661 persons (99.3%) whose annual effective dose was lower than 2 mSv; 43 persons (0.5%) whose annual effective dose per capita was the highest in diagnostic radiology, which was 0.22 mSv; the annual effective dose per capita of radiation workers in primary and secondary hospitals was higher than that in tertiary hospitals; and the abnormal rate of individual dose monitoring was 73. Personnel, accounting for 0.8% of the total number of monitored personnel; the annual effective dose changes of the four types of radiation workers monitored from 2014 to 2018 showed a downward trend, and the annual effective dose of the four types of radiation workers in 2014 was the highest. Conclusion: Personal dosage of radiation workers in some medical institutions in Tianjin is at a low level, and attention should be paid to diagnostic radiology workers.
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Exposición Profesional/estadística & datos numéricos , Dosis de Radiación , Monitoreo de Radiación , Servicio de Radiología en Hospital , China , Humanos , Protección RadiológicaRESUMEN
Artificial selection can provide insights into how insecticide resistance mechanisms evolve in populations. The underlying basis of such phenomena can involve complex interactions of multiple genes, and the resolution of this complexity first necessitates confirmation that specific genes are involved in resistance mechanisms. Here, we used a novel approach invoking a constrained RNA sequencing analysis to refine the discovery of specific genes involved in insecticide resistance. Specifically, for gene discovery, an additional constraint was added to the traditional comparisons of susceptible vs. resistant flies by the incorporation of a line in which insecticide susceptibility was 'recovered' within a resistant line by the removal of insecticide stress. In our analysis, the criterion for the classification of any gene as related to insecticide resistance was based on evidence for differential expression in the resistant line as compared with both the susceptible and recovered lines. The incorporation of this additional constraint reduced the number of differentially expressed genes putatively involved in resistance to 464, compared with more than 1000 that had been identified previously using this same species. In addition, our analysis identified several key genes involved in metabolic detoxification processes that showed up-regulated expression. Furthermore, the involvement of acetylcholinesterase, a known target for modification in insecticide resistance, was associated with three key nonsynonymous amino acid substitutions within our data. In conclusion, the incorporation of an additional constraint using a 'recovered' line for gene discovery provides a higher degree of confidence in genes identified to be involved in insecticide resistance phenomena.
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Resistencia a los Insecticidas/genética , Insecticidas/farmacología , Compuestos Organotiofosforados/farmacología , Tephritidae/efectos de los fármacos , Acetilcolinesterasa/genética , Acetilcolinesterasa/metabolismo , Sustitución de Aminoácidos , Animales , Ontología de Genes , Inactivación Metabólica/genética , Insecticidas/metabolismo , Anotación de Secuencia Molecular , Compuestos Organotiofosforados/metabolismo , Análisis de Secuencia de ARN , Tephritidae/genética , Tephritidae/metabolismo , TranscriptomaRESUMEN
Controlling glycosylation of recombinant proteins produced by CHO cells is highly desired as it can be directed towards maintaining or increasing product quality. To further our understanding of the different factors influencing glycosylation, a glycosylation sub-array of 79 genes and a capillary electrophoresis method which simultaneously analyzes 12 nucleotides and 7 nucleotide sugars; were used to generate intracellular N-glycosylation profiles. Specifically, the effects of nucleotide sugar precursor feeding on intracellular glycosylation activities were analyzed in CHO cells producing recombinant human interferon-gamma (IFN-gamma). Galactose (+/-uridine), glucosamine (+/-uridine), and N-acetylmannosamine (ManNAc) (+/-cytidine) feeding resulted in 12%, 28%, and 32% increase in IFN-gamma sialylation as compared to the untreated control cultures. This could be directly attributed to increases in nucleotide sugar substrates, UDP-Hex ( approximately 20-fold), UDP-HexNAc (6- to 15-fold) and CMP-sialic acid (30- to 120-fold), respectively. Up-regulation of B4gal and St3gal could also have enhanced glycan addition onto the proteins, leading to more complete glycosylation (sialylation). Combined feeding of glucosamine + uridine and ManNAc + cytidine increased UDP-HexNAc and CMP-sialic acid by another two- to fourfold as compared to feeding sugar precursors alone. However, it did not lead to a synergistic increase in IFN-gamma sialylation. Other factors such as glycosyltransferase or glycan substrate levels could have become limiting. In addition, uridine feeding increased the levels of uridine- and cytidine-activated nucleotide sugars simultaneously, which could imply that uridine is one of the limiting substrates for nucleotide sugar synthesis in the study. Hence, the characterization of intracellular glycosylation activities has increased our understanding of how nucleotide sugar precursor feeding influence glycosylation of recombinant proteins produced in CHO cells. It has also led to the optimization of more effective strategies for manipulating glycan quality.
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Metabolismo de los Hidratos de Carbono , Glicoproteínas/metabolismo , Interferón gamma/metabolismo , Nucleótidos/metabolismo , Animales , Células CHO , Cricetinae , Cricetulus , Medios de Cultivo/química , Citidina/metabolismo , Galactosa/metabolismo , Glucosamina/metabolismo , Glicosilación , Hexosaminas/metabolismo , Proteínas Recombinantes/metabolismo , Uridina/metabolismoRESUMEN
Hydrohausmannite nanoparticles (approximately 10 nm) were prepared by the hydrothermal method at 100 degrees C for 72 h. Subsequent annealing was done in air at 400 degrees C and 800 degrees C for 10 h, Mn(3)O(4) nanoparticles (approximately 25 nm) and 3D Mn(2)O(3) porous networks were obtained, respectively. The products were characterized by XRD, TEM, SAED and FESEM. Time-dependent experiments were carried out to exhibit the formation process of the Mn(2)O(3) networks. Their microwave absorption properties were investigated by mixing the product and paraffin wax with 50 vol%. The Mn(3)O(4) nanoparticles possess excellent microwave absorbing properties with the minimum reflection loss of -27.1 dB at 3.1 GHz. In contrast, the Mn(2)O(3) networks show the weakest absorption of all samples. The absorption becomes weaker with the annealing time increasing at 800 degrees C. The attenuation of microwave can be attributed to dielectric loss and their absorption mechanism was discussed in detail.
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Cristalización/métodos , Compuestos de Manganeso/química , Nanoestructuras/química , Nanoestructuras/ultraestructura , Nanotecnología/métodos , Óxidos/química , Absorción , Sustancias Macromoleculares/química , Compuestos de Manganeso/efectos de la radiación , Ensayo de Materiales , Microondas , Conformación Molecular , Nanoestructuras/efectos de la radiación , Óxidos/efectos de la radiación , Tamaño de la Partícula , Porosidad , Dispersión de Radiación , Propiedades de SuperficieRESUMEN
Excessive activity of osteoclasts becomes manifest in many common lytic bone disorders such as osteoporosis, Paget's disease, bone aseptic loosening and tumor-induced bone destruction. Vacuolar proton pump H+-adenosine triphosphatases (V-ATPases), located on the bone-apposed plasma membrane of the osteoclast, are imperative for the function of osteoclasts, and thus are a potential molecular target for the development of novel anti-resorptive agents. To date, the V-ATPases core structure has been well modeled and consists of two distinct functional domains, the V1 (A, B1, B2, C1, C2, D, E1, E2, F, G1, G2, G3, and H subunits) and V0 (a1, a2, a3, a4, d1, d2, c, c' e1, e2 subunits) as well as the accessory subunits ac45 and M8-9. However, the exact configuration of osteoclast specific V-ATPases remains to be established. Inactivation of subunit a3 leads to osteopetrosis in both mice and man because of non-functional osteoclasts that are capable of acidifying the extracellular resorption lacuna. On the other hand, inactivation of subunits c, d1 and ac45 results in early embryonic lethality, indicating that certain subunits, such as a3, are more specific to osteoclast function than others. In osteoclasts, V-ATPases also cooperate with chloride channel protein CLC-7 to acidify the resorption lacuna. In addition, development of V-ATPases inhibitors such as bafilomycin A1, SB 242784 and FR167356 that selectively target osteoclast specific V-ATPases remains a challenge. Understanding the molecular and cellular mechanisms by which specific subunits of V-ATPase regulate osteoclast function might facilitate the development of novel and selective inhibitors for the treatment of lytic bone disorders. This review summarizes recent research developments in V-ATPases with particular emphasis on osteoclast biology.
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Inhibidores Enzimáticos/uso terapéutico , Osteoclastos/enzimología , Osteólisis/tratamiento farmacológico , Osteólisis/enzimología , ATPasas de Translocación de Protón Vacuolares/química , ATPasas de Translocación de Protón Vacuolares/metabolismo , Animales , Humanos , Ratones , Estructura Molecular , Osteoclastos/efectos de los fármacos , ATPasas de Translocación de Protón Vacuolares/antagonistas & inhibidores , ATPasas de Translocación de Protón Vacuolares/genéticaRESUMEN
The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA(3)] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog's (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA(3) or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.