RESUMEN
The immunological responses of activated lymphocytes are associated with increased nitric oxide (NO) biosynthesis. Studies in the literature have primarily approached control of NO by focusing on the regulation of the nitric oxide synthase (NOS) isoforms. However, the present study approaches the control of NO synthesis by addressing the regulation of L-arginine availability to lymphocytes via regulation of membrane transport. The guanidino nitrogen of L-arginine is the sole biosynthetic precursor of NO. We investigated cytokine and mitogen regulation of membrane L-arginine transporters for the first time in feline cells. Feline peripheral blood mononuclear cells were treated with interleukin-2 and concanavalin A, then alternatively spliced isoforms of L-arginine transporters known in other species were probed by RT-PCR, using various oligonucleotide primers that hybridized to several regions in common with the isoforms. Both high affinity and low affinity isoforms are encoded by mRNAs arising from mutually exclusive alternative splicing of the primary transcript. A region of 123 bp was obtained that encoded an extracellular polypeptide loop of 41 amino acids. The sequence of this region represented the high affinity L-arginine substrate binding site of a CAT2 transporter polypeptide isoform, but not the CAT2a isoform low affinity binding site. Neither of the inducible isoforms were constitutively expressed in unstimulated feline cells. This is the first report demonstrating that domestic cats possess the cat2 gene encoding an inducible L-arginine transporter, and, furthermore, that the high affinity isoform transcript is activated by interleukin-2 and concanavalin A in feline lymphocytes.
Asunto(s)
Arginina/farmacocinética , Gatos/inmunología , Concanavalina A/farmacología , Interleucina-2/farmacología , Linfocitos/inmunología , Animales , Arginina/genética , Secuencia de Bases , Sitios de Unión , Transporte Biológico Activo , Datos de Secuencia Molecular , Óxido Nítrico/metabolismo , Isoformas de Proteínas , Transcripción Genética , Regulación hacia ArribaRESUMEN
A new eukaryotic nutrient amino acid transporter has been cloned from an epithelium that is exposed to high voltages and alkaline pH. The full-length cDNA encoding this novel CAATCH1 (cation-anion-activated Amino acid transporter/channel) was isolated using a polymerase chain reaction-based strategy, and its expression product in Xenopus oocytes displayed a combination of several unique, unanticipated functional properties. CAATCH1 electrophysiological properties resembled those of Na(+),Cl(-)-coupled neurotransmitter amine transporters, although CAATCH1 was cloned from a gut absorptive epithelium rather than from an excitable tissue. Amino acids such as l-proline, l-threonine, and l-methionine elicited complex current-voltage relationships in alkaline pH-dependent CAATCH1 that were reminiscent of the behavior of the dopamine, serotonin, and norepinephrine transporters (DAT, SERT, NET) in the presence of their substrates and pharmacological inhibitors such as cocaine or antidepressants. These I-V relationships indicated a combination of substrate-associated carrier current plus an independent CAATCH1-associated leakage current that could be blocked by certain amino acids. However, unlike all structurally related proteins, CAATCH1 activity is absolutely independent of Cl(-). Unlike related KAAT1, CAATCH1 possesses a methionine-inhibitable constitutive leakage current and is able to switch its narrow substrate selectivity, preferring threonine in the presence of K(+) but preferring proline in the presence of Na(+).
Asunto(s)
Proteínas Portadoras/química , Proteínas Portadoras/fisiología , Proteínas de Insectos , Manduca/metabolismo , Proteínas de la Membrana/química , Proteínas de la Membrana/fisiología , Potasio/farmacología , Sodio/farmacología , Secuencia de Aminoácidos , Animales , Proteínas Portadoras/genética , Cloruros/farmacología , Clonación Molecular , ADN Complementario , Sistema Digestivo/metabolismo , Células Epiteliales/metabolismo , Femenino , Biblioteca de Genes , Glicosilación , Concentración de Iones de Hidrógeno , Larva , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Proteínas de la Membrana/genética , Metionina/farmacología , Modelos Moleculares , Datos de Secuencia Molecular , Oocitos/fisiología , Fosforilación , Prolina/metabolismo , Conformación Proteica , Alineación de Secuencia , Homología de Secuencia , Transcripción Genética , Xenopus laevisRESUMEN
We characterized the development and pharmacology of Ca(2+) channel currents in NGF-treated embryonic day 21 cultured rat septal cells. Using standard whole-cell voltage clamp techniques, cells were held at -80 mV and depolarized to construct current-voltage relations in conditions that eliminated Na(+) or K(+) currents. Barium (10 mM) was used as the charge carrier. Maximum current was produced when cells were depolarized to 0 or +10 mV. Recordings from 77 cells revealed that Ca(2+) channel current density increases over time in culture from nearly 0 pA/pF on day 2 in vitro (0.65+/-0.65 pA/pF) to (6.95+/-1.59 pA/pF) on days 6-8. This was followed by a period where currents became nearly 3 times more dense (21.05+/-7.16 pA/pF) at days 9-17. There was little or no evidence for low voltage activated currents. Bath application of 50-100 microM CdCl(2) abolished approximately 95% of the current. Application of 10 microM nimodipine produced a 50.5+/-3.22% reduction in current, 2 microM omega-CTx-GVIA produced a 32.4+/-7.3% reduction, and application of 4 microM omega-Aga-IVA produced a 29.5+/-5.73% reduction in current. When all three inhibitors (10 microM nimodipine, 2 microM omega-CTx-GVIA, and 4 microM omega-Aga-IVA) were applied simultaneously, a residual current remained that was 18.0+/-4.9% of the total current and was completely abolished by application of CdCl(2). This is the first report to characterize Ca(2+) channel currents in cultured embryonic septal cells. These data indicate that there is a steady increase in Ca(2+) channel expression over time in vitro, and show that like other cultured neuronal cells, septal cells express multiple Ca(2+) channel types including L, N, P/Q and R-type channels.
Asunto(s)
Canales de Calcio/fisiología , Tabique Pelúcido/embriología , Animales , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Células Cultivadas , Senescencia Celular/fisiología , Conductividad Eléctrica , Embrión de Mamíferos/citología , Embrión de Mamíferos/metabolismo , Nimodipina/farmacología , Ratas , Ratas Long-Evans , omega-Agatoxina IVA/farmacología , omega-Conotoxina GVIA/farmacologíaRESUMEN
Multipotential progenitor cells grown from central nervous system (CNS) tissues in defined media supplemented with epidermal growth factor (EGF), when attached to a suitable substratum, differentiate to express neural and glial histochemical markers and morphologies. To assess the functional characteristics of such cells, expression of voltage-gated Na+ and K+ currents (INa, IK) was studied by whole-cell patch clamp methods in progenitors raised from postnatal rat forebrain. Undifferentiated cells were acutely dissociated from proliferative "spheres," and differentiated cells were studied 1-25 days after plating spheres onto polylysine/laminin-treated coverslips. INa and IK were detected together in 58%, INa alone in 11%, and IK alone in 19% of differentiated cells recorded with K(+)-containing pipettes. With internal Cs+ (to isolate INa), INa up to 45 pA/pF was observed in some cells within 1 day after plating. I Na ranged up to 150 pA/pF subsequently. Overall, 84% of cells expressed I Na, with an average of 38 pA/pF. INa had fast kinetics, as in neurons, but steadystate inactivation curves were strongly negative, resembling those of glial INa. Inward tail currents sensitive to [K+]out were observed upon repolarization after the 10-ms test pulse with internal Cs+, indicating the expression of K+ channels in 82% of cells. In contrast to the substantial currents observed in differentiating cells, little or no INa or Ik-tail currents were detected in recordings from cells acutely dissociated from spheres. Thus, in the presence of EGF, ionic currents develop early during differentiation induced by attachment to an appropriate substratum. Cells switched from EGF to basic fibroblast growth factor (bFGF) when plated onto coverslips showed greatly reduced proliferation and developed less neuron-like morphologies than cells plated in the presence of EGF. INa was observed in only 53% of bFGF-treated cells, with an average of 9 pA/pF. Thus, in contrast to reports that bFGF promotes neuronal differentiation in some CNS progenitor populations, our EGF-generated postnatal rat CNS progenitors do not develop neuronal characteristics when switched to medium containing bFGF. Thus, differentiated CNS progenitors can express a mix of neuronal and glial molecular, morphological, and electrophysiological properties that can be modified by culture conditions.
Asunto(s)
Sistema Nervioso Central/citología , Factor de Crecimiento Epidérmico/farmacología , Canales Iónicos/fisiología , Células Madre/química , Animales , Animales Recién Nacidos , Fenómenos Biofísicos , Biofisica , Química Encefálica/fisiología , Adhesión Celular/efectos de los fármacos , Adhesión Celular/fisiología , Diferenciación Celular , Células Cultivadas/citología , Células Cultivadas/efectos de los fármacos , Células Cultivadas/fisiología , Medios de Cultivo/farmacología , Electrofisiología , Estudios de Evaluación como Asunto , Activación del Canal Iónico/fisiología , Canales de Potasio/efectos de los fármacos , Canales de Potasio/fisiología , Prosencéfalo/citología , Ratas , Canales de Sodio/efectos de los fármacos , Canales de Sodio/fisiología , Células Madre/citología , Células Madre/fisiologíaRESUMEN
N-type calcium channels are omega-conotoxin (omega-CgTx)-sensitive, voltage-dependent ion channels involved in the control of neurotransmitter release from neurons. Multiple subtypes of voltage-dependent calcium channel complexes exist, and it is the alpha 1 subunit of the complex that forms the pore through which calcium enters the cell. The primary structures of human neuronal calcium channel alpha 1B subunits were deduced by the characterization of overlapping complementary DNAs. Two forms (alpha 1B-1 and alpha 1B-2) were identified in human neuroblastoma (IMR32) cells and in the central nervous system, but not in skeletal muscle or aorta tissues. The alpha 1B-1 subunit directs the recombinant expression of N-type calcium channel activity when it is transiently co-expressed with human neuronal beta 2 and alpha 2b subunits in mammalian HEK293 cells. The recombinant channel was irreversibly blocked by omega-CgTx but was insensitive to dihydropyridines. The alpha 1B-1 alpha 2b beta 2-transfected cells displayed a single class of saturable, high-affinity (dissociation constant = 55 pM) omega-CgTx binding sites. Co-expression of the beta 2 subunit was necessary for N-type channel activity, whereas the alpha 2b subunit appeared to modulate the expression of the channel. The heterogeneity of alpha 1B subunits, along with the heterogeneity of alpha 2 and beta subunits, is consistent with multiple, biophysically distinct N-type calcium channels.
Asunto(s)
Canales de Calcio/efectos de los fármacos , Canales de Calcio/genética , Canales de Calcio/metabolismo , Péptidos Cíclicos/farmacología , Secuencia de Aminoácidos , Calcio/metabolismo , Línea Celular , Femenino , Humanos , Masculino , Potenciales de la Membrana , Datos de Secuencia Molecular , Neuroblastoma/metabolismo , Alineación de Secuencia , Homología de Secuencia de Ácido Nucleico , Transfección , omega-Conotoxina GVIARESUMEN
The primary structures of human neuronal alpha 1, alpha 2, and beta subunits of a voltage-dependent Ca2+ channel were deduced by characterizing cDNAs. The alpha 1 subunit (alpha 1D) directs the recombinant expression of a dihydropyridine-sensitive L-type Ca2+ channel when coexpressed with the beta (beta 2) and the alpha 2 (alpha 2b) subunits in Xenopus oocytes. The recombinant channel is also reversibly blocked by 10-15 microM omega-conotoxin. Expression of the alpha 1D subunit alone, or coexpression with the alpha 2b subunit, did not elicit functional Ca2+ channel activity. Thus, the beta 2 subunit appears to serve an obligatory function, whereas the alpha 2b subunit appears to play an accessory role that potentiates expression of the channel. The primary transcripts encoding the alpha 1D, alpha 2, and beta subunits are differentially processed. At least two forms of neuronal alpha 1D were identified. Different forms of alpha 2 and beta transcripts were also identified in CNS, skeletal muscle, and aorta tissues.
Asunto(s)
Canales de Calcio/genética , Neuronas/química , Secuencia de Aminoácidos , Animales , Canales de Calcio/química , Canales de Calcio/fisiología , Clonación Molecular , ADN/química , ADN/genética , Dihidropiridinas/farmacología , Expresión Génica , Humanos , Sustancias Macromoleculares , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Oocitos/metabolismo , Reacción en Cadena de la Polimerasa , ARN Mensajero/análisis , Distribución Tisular , Transcripción Genética , Xenopus laevis/metabolismoRESUMEN
B cells and C cells in frog lumbar sympathetic ganglia are specifically innervated by preganglionic B fibers and C fibers, respectively. To explore the mechanisms underlying the formation of these specific synapses, electrophysiological studies were made of sprouting and regenerating synaptic connections following interruption of the preganglionic pathways. Studies were also made of developing connections in tadpole ganglia. After partial denervation (by selective interruption of B fibers), the C fibers sprouted and innervated B cells. When B fibers regenerated, they reinnervated B cells only, and within several weeks, C fiber synapses on B cells were no longer found. After complete denervation (by interruption of both B and C fibers) specific synaptic connections were eventually restored. At least 2 experimentally separable processes underlie this specificity: First, there is a preference for appropriate connections from the outset of reinnervation, seen even in the absence of competition between the 2 groups of preganglionic fibers. Despite this preference, however, some inappropriate synapses are formed. Second, those inappropriate synapses that do arise are eliminated when appropriate synapses are allowed to reform, as a result of competitive interactions between the 2 preganglionic fiber groups. In normally developing tadpole ganglia, B and C cells were not readily distinguishable. The great majority of tadpole neurons were found to be innervated exclusively by either B or C fibers. Some neurons were innervated by both preganglionic fiber groups, a situation virtually never found in adult ganglia. It thus appears that in normal development, as in reinnervation, innervation is by and large selective; inappropriate synapses may form, but they are eliminated during maturation, presumably through competitive interactions.
Asunto(s)
Desarrollo Embrionario y Fetal , Ganglios Simpáticos/fisiología , Regeneración Nerviosa , Sinapsis/fisiología , Animales , Desnervación , Larva/fisiología , Fibras Nerviosas/fisiología , Conducción Nerviosa , Rana catesbeiana , Rana pipiens , Valores de Referencia , Factores de TiempoRESUMEN
Blockade of Ca2+ channels by omega-conotoxin GVIA, a 27 amino acid peptide from the venom of the marine snail Conus geographus, was investigated with patch-clamp recordings of whole-cell and unitary currents in a variety of cell types. In dorsal root ganglion neurons, the toxin produces persistent block of L- and N-type Ca2+ channels but only transiently inhibits T-type channels. Its actions appear to be neuron-specific, since it blocks high-threshold Ca2+ channels in sensory, sympathetic, and hippocampal neurons of vertebrates but not in cardiac, skeletal, or smooth muscle cells. Block occurs through direct interaction of the toxin with an external site closely associated with the Ca2+ channel, without apparent involvement of a second messenger or dependence on channel gating. The tissue and channel-type specificity and the directness and slow reversibility of the block are features that favor use of omega-conotoxin as a tool for purifying particular neuronal Ca2+ channels and defining their physiological function.
Asunto(s)
Calcio/metabolismo , Canales Iónicos/fisiología , Venenos de Moluscos/farmacología , Músculos/fisiología , Neuronas/fisiología , Animales , Embrión de Pollo , Canales Iónicos/efectos de los fármacos , Cinética , Músculos/efectos de los fármacos , Neuronas/efectos de los fármacos , omega-Conotoxina GVIARESUMEN
We previously reported that omega Conus geographus toxin (omega CgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion neurons which demonstrate that omega CgTX produces a persistent block of voltage-gated Ca2+ currents. Thus, we conclude that omega CgTX inhibits synaptic transmission by blocking Ca2+ channels in the presynaptic nerve terminal. The toxin had no effect on K+ currents; however, in some but not all neurons, omega CgTX reduced Na+ currents by 10-25%. These findings suggest that omega CgTX should be useful as a probe to examine synaptic Ca2+ channels.
Asunto(s)
Calcio/metabolismo , Canales Iónicos/efectos de los fármacos , Venenos de Moluscos/farmacología , Animales , Células Cultivadas , Ganglios Espinales/efectos de los fármacos , Ganglios Espinales/metabolismo , Canales Iónicos/metabolismo , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Péptidos/farmacología , Potasio/metabolismo , Sodio/metabolismo , Sinapsis/efectos de los fármacos , Sinapsis/metabolismo , omega-Conotoxina GVIAAsunto(s)
Desarrollo Infantil , Niño Superdotado/psicología , Familia , Niño , Historia del Siglo XX , Humanos , Judaísmo/historia , Música , U.R.S.S. , Estados UnidosAsunto(s)
Niño Superdotado , Logro , Arte , Niño , Niño Superdotado/educación , Preescolar , Humanos , Inteligencia , Relaciones Interpersonales , Matemática , Destreza Motora , Lectura , CienciaRESUMEN
Data on changes in children's map drawing ability are used to examine 3 hypotheses concerning the relationship of level mixture to developmental change as proposed by Damon in a recent study of social reasoning: (1) that high total mixture precedes change, (2) that low total mixture precedes change, and (3) that high positive mixture (i.e., that at stages above the mode) precedes change. Further predictions are derived from our 6-phase model of transition; these are compared and contrasted with conclusions reached by Damon. Using Damon's approach, we replicate his finding that high positive mixture precedes model advance. More detailed analyses of 2 separate groups as suggested by the transitions model, however, show that all 3 types of mixture precede developmental change, albeit different types of change within each group.
Asunto(s)
Desarrollo Infantil , Cognición , Percepción Espacial , Niño , Humanos , Orientación , Solución de ProblemasRESUMEN
The basal lamina (BL) that occupies the synaptic cleft of the skeletal neuromuscular junction is antigenically distinct from extrasynaptic muscle fiber BL, rich in acetylcholinesterase (AChE), and bears projections that form junctional folds in the postsynaptic membrane. We report here that these synapse-specific features of BL are all present at low levels in embryonic rat myotubes cultured without nerve, and that their levels are markedly increased by addition of a soluble extract from adult rat brain. Light and electron microscopic methods show that: (1) antibodies which bind preferentially to synaptic BL in vivo stain small, discrete patches of the myotube's BL; (2) AChE accumulates in patches on the myotube surface; and (3) myotube BL and membrane form invaginations that resemble junctional folds. Patches of BL that bear synaptic antigens, AChE, or folds usually overlie clusters of acetylcholine receptors in the plasma membrane. Myotubes treated with a brain extract bear 5 to 20 times more junctional folds and patches rich in acetylcholine receptors, synaptic BL antigens, and AChE than control myotubes. Together with a previous demonstration that electrical and/or contractile activity can modulate the amount and composition of myotube BL (Sanes, J.R., and J.C. Lawrence, Jr. (1983) Dev. Biol. 97: 123-136), these results suggest that nerves could regulate differentiation of muscle fiber BL by a combination of activity-dependent and -independent mechanisms.