RESUMEN
Examination of carnosine in patients with diabetes mellitus type I, showed that the plasma levels of carnitine were non significantly increased compared to the levels in healthy population, while the levels in red cells were decreased Lowered levels of carnosine in red cells could point out similar deficit in other cells. Due to low levels in cells carnosine is less available for metabolic processes, like antioxidant reactions and its participation in antioxidants defense reactions is limited non-enzymatic glycosylation of proteins. Therefore it should be supplemented. (Tab. 1, Fig. 1, Ref. 15.)
Asunto(s)
Carnosina/sangre , Diabetes Mellitus Tipo 1/sangre , Adulto , Carnosina/orina , Diabetes Mellitus Tipo 1/orina , Eritrocitos/química , Femenino , Humanos , MasculinoRESUMEN
The properties of the complex of ferricyt c with fluorosulfonated polyanion Nafion (as a representative 'hydrophobic' polyanion) have been studied by means of optical spectroscopy and differential scanning calorimetry. The addition of the polyanion to a solution of ferricyt c (pH 7.4) resulted in an expansion of the protein molecule characterized by a profound decrease in enthalpy of the thermal transition of ferricyt c. The conformational change of ferricyt c upon addition of Nafion was shown by a perturbation of the Met-80-heme iron bond and an apparent increase in the distance of Trp-59 from the heme. The conformational change in the heme region was also observed by examining the CD spectra. The conformational state of ferricyt c in a complex with Nafion was similar to that designated as state II by Hildebrandt (Hildebrandt, P. (1990) Biochim. Biophys. Acta 1040, 175-186) in the complex with negatively charged heteropolytungstates-a six-coordinated low-spin state with a destabilized structure of the heme crevice. The decrease in enthalpy of the thermal transition of ferricyt c, the spectral changes in absorbance and the CD spectra, together with an increase in Trp fluorescence induced by Nafion addition observed at high ionic strength, all point to the involvement of the non-coulombic interaction.