RESUMEN
Production of alpha-amylase with B. subtilis CCM 2722 in an aqueous two-phase polyethylene glycol/dextran system integrated with product purification by affinity chromatography on crosslinked starch during cultivation was studied. The medium was drawn from the bioreactor to the external settler during fermentation. After phase separation in the settler the dextran-rich bottom phase with cells was returned to the bioreactor. The PEG-rich top phase was pumped to the column with crosslinked starch and returned to the bioreactor after alpha-amylase adsorption. The same volumetric productivities, 0.53 U/mL/h, were reached in both batch and described process, but total productivity of the latter method was much higher owing to shortening upstream and downstream processing time. The enzyme of 98% homogenity in 95% yield was obtained after its elution from the column.
Asunto(s)
Bacillus subtilis/enzimología , alfa-Amilasas/biosíntesis , Cromatografía de Afinidad , Reactivos de Enlaces Cruzados , Medios de Cultivo , Dextranos , Polietilenglicoles , Almidón , alfa-Amilasas/aislamiento & purificaciónRESUMEN
Juvenile chronic arthritis implies in almost one third of the patients a disease with permanent sequelae--deformities of the joints with subsequent disability or the affection of various organs. To prevent the development of such changes, comprehensive treatment of these patients is necessary comprising coordinated medicamentous rehabilitation and physical therapy as well as psychosocial care. Collaboration of the doctor, patient, parents and social worker is one of the prerequisites of an optimal perspective.