RESUMEN
Changes in photosynthetic machinery can induce physiological and biochemical damage in plants. Low doses of glyphosate have been shown to exert a positive effect in mitigating the deleterious effects of water deficit in plants. Here, the physiological and biochemical mechanisms of safflower plants (Carthamus tinctorius L.) were studied under conditions of water deficit mediated by the attenuating effect of low-dose glyphosate. The plants were divided into two groups of water regimes in soil, without water deficit (-10 kPa) and with water deficit (-70 kPa), and were exposed to different concentrations of glyphosate (0, 1.8, 3.6, 7.2, 18, 36, 72, 180, 360, and 720 g a.e. ha-1). Evident protective responses at the physiological and biochemical levels were obtained after applying low doses of glyphosate to plants under water deficit, with a limiting dose for the occurrence of hormesis (LDS) = 72 g a.e. ha-1. The water deficit in plants resulted in hydrogen peroxide (H2O2) accumulation and consequently lipid peroxidation (LPO) associated with the accumulation of shikimic acid and glyphosate in plants, which triggered an increase in the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) that act by dismuting the levels of reactive oxygen species (ROS), maintaining, and/or increasing the maximum quantum efficiency of photosystem II (Fv/Fm), effective quantum yield of photosystem II (ΦPSII), electron transport rate (ETR), photochemical extinction coefficient (qP), and non-photochemical extinction coefficient (NPQ). APX appears to be the main enzyme involved in eliminating H2O2. Low doses of glyphosate act as water deficit ameliorators, allowing the plant to maintain/increase metabolism at physiological and biochemical levels by activating antioxidant enzymes in the dismutation of ROS in safflower plants.
Asunto(s)
Carthamus tinctorius , Antioxidantes/metabolismo , Carthamus tinctorius/metabolismo , Glicina/análogos & derivados , Hormesis , Peróxido de Hidrógeno , Fotosíntesis , Estrés Fisiológico , Agua , GlifosatoRESUMEN
To meet the growing demand for soybean it is necessary to increase crop yield, even in low water availability conditions. To circumvent the negative effects of water deficit, application of biostimulants with anti-stress effect has been adopted, including products based on fulvic acids and Ascophyllum nodosum (L.) seaweed extracts. In this study, we determined which formulation and dosage of a biostimulant is more efficient in promoting the recovery of soybean plants after stress due to water deficit. The experiment was conducted in a greenhouse, in a double-factorial randomized block design with two additional factors, four repetitions and eleven treatments consisting of three biostimulant formulations (F1, F2 and F3), and three dosages (0.25; 0.50 and 1.0 kg ha-1); a control with water deficit and a control without water deficit. Soybean plants were kept at 50% of the pot's water capacity for three days, then rehydrated and submitted to the application of treatments with biostimulant. After two days of recovery, growth, physiological, biochemical and yield parameters were evaluated. All plants that received the application of the biostimulant produced more than the water-stressed control plants. The biostimulant provided higher photosynthetic rates, more efficient mechanisms for dissipating excess energy and higher activities of antioxidant enzymes. Plants treated with biostimulant were more efficient in the recovery of the metabolic activities after rewatering, resulting in increased soybean tolerance to water deficit and reduced yield losses. The best result obtained was through the application of formulation 2 of the biostimulant at a dosage of 0.25 kg ha-1.