RESUMEN
Anti-fungi activity of organic extracts from the tree Fagara monophylla (Rutaceae) in Venezuela. The tree Fagara monophylla ranges throughout Tropical America. The genus Fagara has a diversity of alkaloid compounds with antibiotic properties; nevertheless, there are few reports antifungal activity of its organic compounds. Organic extracts from Venezuelan F. monophylla were tested for antimicrobial activity against Aspergillus terreus, A. flavus, Penicillium digitatum, P. funiculosum, P. citrinum, Paecilomyces and Candida albicans. Minimal Inhibitory Concentration (MIC) was determined. The susceptibility trials of organic fractions (Hex., CH2Cl2 and MeOH) showed that the highest inhibition was presented by MeOH against A. flavus (55 mm), P. digitatum (60 mm), P. funiculosum (56 mm) and C. albicans (26 mm). The activities of MeOH/EtOAc fractions 1 and 2 suggest a combined effect against A. flavus, P. digitatum and P. funiculosum. The MIC of 1 MeOH/ EtOAc subfraction activity was lower against C. albicans (32 µg/ml) and moderate (128 µg/ml) against P. digitatum. This organic extract has a great antifungal potential. The phytochemical proves and TLC testing on the organic extract, and the MeOH/EtOAc subfraction, respectively, indicated the presence of alkaloid compounds. Rev. Biol. Trop. 55 (3-4): 767-775. Epub 2007 December, 28.
Fagara monophylla está distribuida en América Tropical. El género Fagara tiene diversidad de compuestos alcaloides con propiedades antibacterianas; sin embargo existe escasa información acerca de su actividad antifúngica. Evaluamos extractos de F. monophylla in vitro con los hongos Aspergillus terreus, A. flavus, Penicillium digitatum, P. citrinum, P. funiculosum, Paecilomyces y Candida albicans determinando la Concentración Mínima Inhibitoria (CMI). Hicimos pruebas de susceptibilidad con las fracciones Hex., CH2Cl2 y la MeOH. La mayor inhibición la presentó la fracción MeOH frente a A. flavus (55 mm), P. digitatum (60 mm), P. funiculosum (56 mm) y C. albicans (26 mm). Las actividades mostradas por las fracciones 1 y 2 de MeOH/EtOAc sugieren la acción de un efecto combinado frente a A flavus, P, digitatum y P. funiculosum. La CMI de la subfracción 1 MeOH/EtOAc frente a Candida albicans fue baja (32 µg/ml), y para P. digitatum la CMI fue moderada (128 µg/ml). Este extracto orgánico posee gran potencial de actividad antifúngica. Las pruebas fitoquímicas y los ensayos realizados por TLC al extracto orgánico y a la subfracción 1 MeOH/EtOAc, respectivamente, mostraron la presencia de alcaloides.
Asunto(s)
Antifúngicos/farmacología , Hongos Mitospóricos/efectos de los fármacos , Extractos Vegetales/farmacología , Rutaceae/química , Pruebas de Sensibilidad Microbiana , VenezuelaRESUMEN
The tree Fagara monophylla ranges throughout Tropical America. The genus Fagara has a diversity of alkaloid compounds with antibiotic properties; nevertheless, there are few reports antifungal activity of its organic compounds. Organic extracts from Venezuelan F. monophylla were tested for antimicrobial activity against Aspergillus terreus, A. flavus, Penicillium digitatum, P. funiculosum, P. citrinum, Paecilomyces and Candida albicans. Minimal Inhibitory Concentration (MIC) was determined. The susceptibility trials of organic fractions (Hex., CH2Cl2 and MeOH) showed that the highest inhibition was presented by MeOH against A. flavus (55 mm), P. digitatum (60 mm), P. funiculosum (56 mm) and C. albicans (26 mm). The activities of MeOH/EtOAc fractions 1 and 2 suggest a combined effect against A. flavus, P. digitatum and P. funiculosum. The MIC of 1 MeOH/ EtOAc subfraction activity was lower against C. albicans (32 microg/ml) and moderate (128 microg/ml) against P. digitatum. This organic extract has a great antifungal potential. The phytochemical proves and TLC testing on the organic extract, and the MeOH/EtOAc subfraction, respectively, indicated the presence of alkaloid compounds.