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Carbohydr Polym ; 113: 607-14, 2014 Nov 26.
Artículo en Inglés | MEDLINE | ID: mdl-25256524

RESUMEN

The human genome encodes a gene for an enzymatically active chitinase (CHIT1) located in a single copy on Chromosome 1, which is highly expressed by activated macrophages and in other cells of the innate immune response. Several dysfunctional mutations are known in CHIT1, including a 24-bp duplication in Exon 10 causing catalytic deficiency. This duplication is a common variant conserved in many human populations, except in West and South Africans. Thus it has been proposed that human migration out of Africa and the consequent reduction of exposure to chitin from environmental factors may have enabled the conservation of dysfunctional mutations in human chitinases. Our data obtained from 85 indigenous Amerindians from Peru, representative of populations characterized by high prevalence of chitin-bearing enteroparasites and intense entomophagy, reveal a very high frequency of the 24-bp duplication (47.06%), and of other single nucleotide polymorphisms which are known to partially affect enzymatic activity (G102S: 42.7% and A442G/V: 25.5%). Our finding is in line with a founder effect, but appears to confute our previous hypothesis of a protective role against parasite infection and sustains the discussion on the redundancy of chitinolytic function.


Asunto(s)
Quitina/química , Hexosaminidasas/genética , Inmunidad Innata/genética , Animales , Quitina/genética , Dieta , Hexosaminidasas/deficiencia , Humanos , Indígenas Sudamericanos , Macrófagos/metabolismo , Macrófagos/parasitología , Mutación , Parásitos/química , Parásitos/metabolismo , Perú , Polimorfismo de Nucleótido Simple
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