RESUMEN

The essential role of zinc in embryogenesis was identified through studies of its presence in eggs and embryos, the effects of its deficiency and its role in metallo proteins required for organ development and formation. The Xenopus laevis oocyte zinc content varies during oogenesis. It increases from 3 to 70 ng zinc/oocyte as it progresses from stage I to VI. The oocyte zinc is derived from the maternal liver as part of a metallo-complex with vitellogenin. The latter transports the metal in plasma and into the oocyte. Once internalized, most of the zinc is stored within yolk platelets bound to lipovitellin, one of the processed products of vitellogenin. About 90% of the total zinc is associated with the yolk platelet lipovitellin while the remaining 10% is in a compartment associated with hitherto unknown molecule(s) or organelle(s) of the cytoplasm. The bi-compartmental distribution remains constant throughout embryogenesis since the embryo behaves as a closed system for zinc after fertilization. The yolk platelet zinc is used after the tadpole is hatched while we proposed that the 10% of the zinc in the non-yolk platelet pool is the one used for embryogenesis. It provides zinc to newly synthesized molecules responsible for the development of zinc-dependent organ genesis. Interference with the availability of this zinc by the chelating agent 1,10-phenanthroline results in the development of embryos that lack dorsal organs, including brain, eyes and spinal cord. The extensive teratology is proposed to be due to altered or absent zinc distribution between the cytosolic pool and zinc-transcription factors. The data identify the components of a zinc transport, storage and distribution system in a vertebrate organism.

Asunto(s)

Embrión de Mamíferos/fisiología , Oocitos/fisiología , Zinc/fisiología , Animales , Núcleo Celular/metabolismo , Desarrollo Embrionario y Fetal/fisiología , Femenino , Humanos , Transporte Iónico , Oogénesis/fisiología , Factores de Transcripción/metabolismo , Vitelogeninas/metabolismo , Zinc/química , Zinc/deficiencia

RESUMEN

Chicken alpha- and beta-lipovitellin are derived from parent vitellogenin proteins and contain four subunits (125, 80, 40, and 30 kDa) and two subunits (125 and 30 kDa), respectively. Metal analyses demonstrate both are zinc proteins containing 2.1 +/- 0.2 mol of zinc/275 kDa per alpha-lipovitellin and 1.4 +/- 0.2 mol of zinc/155 kDa per beta-lipovitellin, respectively. The subunits of beta-lipovitellin, Lv 1 (MW 125 kDa) and Lv 2 (MW 30 kDa), are separated by gel exclusion chromatography in the presence of zwittergent 3-16. Zinc elutes with Lv 1, suggesting that this subunit binds zinc in the absence of Lv 2. The subunits of alpha- and beta-lipovitellin were separated by SDS-PAGE, digested with trypsin, and mapped by reverse-phase HPLC. The peptide maps of the 125-kDa subunits from alpha- and beta-lipovitellin are essentially identical. Similar results are obtained for the 30-kDa subunits of both lipovitellins. The sequences of five and four peptides of the 125-kDa subunit of alpha- and beta-Lv, respectively, and two peptides of the 30-kDa subunit of alpha- and beta-lipovitellin were determined and match those predicted from the gene for vitellogenin II, Vtg II. Comparison of the amino acid composition of the 125- and 30-kDa subunits of alpha- and beta-lipovitellin support the conclusion that they originate from the same gene. The sequences of peptides from the 80- and 40-kDa subunits of alpha-lipovitellin have not been found in the NCBI nonredundant data bank. The 27-amino acid N-terminal sequence of the 40-kDa protein is 56% similar to the last third of the Lv 1-coding region of the Vtg II gene, suggesting it may come from an analogous region of the Vtg I gene. We propose a scheme for the precursor-product relationship of Vtg I.

Asunto(s)

Proteínas Dietéticas del Huevo/análisis , Zinc/análisis , Aminoácidos/análisis , Animales , Pollos , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Proteínas del Huevo , Electroforesis en Gel de Poliacrilamida , Mapeo Peptídico , Homología de Secuencia de Aminoácido , Xenopus laevis

RESUMEN

The embryonic epidermis of stage 35 Xenopus laevis embryos is a highly differentiated structure composed of four cell types arranged in a regular architecture. Each type is distinguished by its distinct morphological characteristics. Some cells are ciliated (type 1); others have their surfaces covered by abundant, secreted vesicles of 0.1 microm diameter (type 2), or multiple linear aggregates of spherical subunits on their apical surfaces (type 3) or large secreted vesicles that emanate from prominent apical holes of 1 microm diameter (type 4). In contrast, the macroscopic appearance of embryos exposed to 10 microM 1,10-phenanthroline (OP) as well as the ultramicroscopic structure and organization of their epidermal cells are markedly altered. The most predominant cells of the embryonic epidermis are undifferentiated and of heterogeneous size. They lack any characteristic morphology and are arranged irregularly. Ghost cells are also identified. The recognizable differentiated cells are decreased in number and present in a scattered arrangement. These are identified as either type 1 or 2 cells but with ciliae that are shorter and thicker than control or with only a few vesicles larger than 0.1 microm in diameter on their surface. No cells with linear aggregates or prominent apical holes are identified. Except for the altered epidermis, the embryos do not develop any other major organs and exhibit axial abnormalities with an average dorso-anterior index of three. Thus, the chelating agent OP perturbs metal dependent processes essential for terminal differentiation that may likely account for the resultant abnormalities of embryo organogenesis and morphogenesis.

Asunto(s)

Diferenciación Celular/efectos de los fármacos , Quelantes/farmacología , Células Epidérmicas , Epidermis/efectos de los fármacos , Fenantrolinas/farmacología , Xenopus laevis/embriología , Animales , Embrión no Mamífero/ultraestructura , Epidermis/ultraestructura , Microscopía Electrónica de Rastreo , Factores de Tiempo

RESUMEN

Zinc regulates the gene expression machinery. It affects the structure of chromatin, the template function of its DNA, the activity of numerous transcription factors and of RNA polymerases. Hence, it determines both the types of mRNA transcripts synthesized and the rate of transcription itself. Alterations in one or more of these zinc dependent processes have been proposed to account for the proliferative arrest and teratology induced by zinc deficiency. To examine this proposal, studies of zinc during X. laevis development have been initiated. The kinetics of X. laevis oocyte zinc uptake and storage and of zinc utilization during embryogenesis have been examined first. Vitellogenin carries zinc into the oocyte. Ten % of the total zinc (10 ng/egg) remains within the cytosol while 90% (90 ng/egg) is stored in the yolk platelets associated with lipovitellin. The cytosolic pool is the source of the zinc for all newly formed metalloproteins involved in embryo development. The yolk platelet zinc pool is stored for later use during early metamorphosis. It is now possible to examine zinc transfer to molecules, such as e.g. transcription factors, and the role of the metal in their function in development and organogenesis.

Asunto(s)

Regulación de la Expresión Génica , Morfogénesis/genética , Zinc/fisiología , Animales , Cromatina/química , Cromatina/metabolismo , Cromatina/fisiología , Regulación de la Expresión Génica/efectos de los fármacos , Humanos , Morfogénesis/efectos de los fármacos , Factores de Transcripción/química , Factores de Transcripción/metabolismo , Factores de Transcripción/fisiología

RESUMEN

The x-ray absorption fine structure (XAFS) zinc K-edge steps for intact stages I,II and V,VI Xenopus laevis oocytes demonstrate that the zinc concentration is about 3 and 1 mM, respectively. However, the chi(k) function for the early stage oocytes differs markedly from that for the late one. Analysis of the XAFS data for stage I,II oocytes indicates that zinc is bound to 2.0 +/- 0.5 sulfur atoms at an average coordination distance of 2.29 +/- 0.02 angstroms and 2.0 +/- 0.5 nitrogen or oxygen (N/O) atoms at 2.02 +/- 0.02 angstroms. In marked contrast, in stage V,VI oocytes, zinc is bound to 4.1 +/- 0.4 N/O atoms at an average distance of 1.98 +/- 0.01 angstroms. Our previous studies demonstrated that 90% of the zinc in stage VI oocytes is sequestered within yolk platelets, associated with a single molecule, lipovitellin, the proteolytically processed product of vitellogenin. XAFS analysis of yolk platelets, lipovitellin, and vitellogenin demonstrates that zinc is bound to 4.0 +/- 0.5 N/O ligands at an average distance of 1.98 +/- 0.01 angstroms in each case, identical to that of stage V,VI oocytes. The higher shell contributions in the Fourier transforms indicate that two of the N/O zinc ligands are His in both stage V,VI and I,II oocytes. The results show that in stage I,II oocytes, there is a high concentration of a zinc protein whose zinc coordination site likely is composed of (His)2(Cys)2, such as, e.g., TFIIIA. As the oocytes develop, the predominant zinc species becomes one that exhibits the (His)2(N/0)2 zinc site found in lipovitellin. Hence, the ligands to the zinc atoms in intact oocytes and the changes that take place as a function of oogenesis and after their fertilization, during embryogenesis, now can be examined and explored.

Asunto(s)

Oocitos/metabolismo , Oogénesis/fisiología , Zinc/metabolismo , Secuencia de Aminoácidos , Animales , Sitios de Unión , Proteínas del Huevo , Proteínas Dietéticas del Huevo/genética , Proteínas Dietéticas del Huevo/metabolismo , Femenino , Técnicas In Vitro , Ligandos , Metaloendopeptidasas/genética , Metaloendopeptidasas/metabolismo , Datos de Secuencia Molecular , Oocitos/crecimiento & desarrollo , Espectrometría por Rayos X , Vitelogeninas/genética , Vitelogeninas/metabolismo , Xenopus laevis

Asunto(s)

Prótesis de Cadera/efectos adversos , Prótesis de la Rodilla/efectos adversos , Enfermedades Linfáticas/etiología , Trasplante Óseo , Femenino , Reacción a Cuerpo Extraño/etiología , Histiocitosis Sinusal/etiología , Humanos , Hiperplasia , Ganglios Linfáticos/patología , Linfadenitis/etiología , Macrófagos/patología , Persona de Mediana Edad , Polietilenos/efectos adversos , Falla de Prótesis , Reoperación , Propiedades de Superficie

RESUMEN

Xenopus laevis vitellogenin contains 2 g-atoms (g-at) of Zn and 3 g-at of Ca/dimer, transports zinc in plasma, and plays a role in its distribution within the oocyte [Montorzi et al. (1994) Biochem. Biophys. Res. Commun. 200, 1407-1413; Montorzi et al. (1995) Biochemistry 34, 10851-10858]. We here report the dynamics and time course of Zn65-labeled vitellogenin uptake by and distribution within stages II and IV oocytes, the fate of the metal in oocytes as they progress from stages II to VI, as well as in the first two cleavage blastomeres, the blastula, and subsequent stages of the developing embryo and tadpole. Zn65 bound to vitellogenin is taken up within less than 30 min by either stage II or IV oocytes incubated under in vitro culture conditions whereas free Zn65 is not. Once internalized, Zn65 remains within the cytosol of stage II, whereas in stage IV oocytes, it is transferred within 4 h of its entry from the cytosol into yolk platelets. Nearly all of the transferred Zn65 is found within yolk platelets and their precursors where it is associated with the vitellogenin cleavage product, lipovitellin. Its distribution within the oocyte organelles differs at each stage of oogenesis. In the early stages (III-IV) most of the oocyte zinc is located first in the small endocytosed vesicles and then in multivesicular bodies. When the zinc transfer process is finalized in the late stages of oogenesis (V-VI), > 90% of the total oocyte zinc is within yolk platelets while the remainder is in the cytosol. In embryos and tadpoles, the larger of these two pools remain sequestered in yolk platelets and is inaccessible to cytosolic apoproteins throughout the entire period of embryo formation. Its redistribution to the cytosol does not begin until several days after the tadpole has hatched. The smaller pool, on the other hand, is already present in the cytosol and is, therefore, postulated to constitute the sole source of zinc required for embryogenesis.

Asunto(s)

Embrión no Mamífero/metabolismo , Metaloproteínas/metabolismo , Oocitos/metabolismo , Vitelogeninas/metabolismo , Zinc/metabolismo , Animales , Transporte Biológico , Blastómeros/metabolismo , Compartimento Celular , Citosol/metabolismo , Yema de Huevo/metabolismo , Oocitos/crecimiento & desarrollo , Distribución Tisular , Xenopus laevis/embriología , Xenopus laevis/metabolismo , Radioisótopos de Zinc

RESUMEN

Xenopus laevis vitellogenin is a plasma protein that contains a total of 5 mol of metal/440 kDa dimer, 2 mol of zinc, and 3 mol of calcium (Montorzi et al. (1994) Biochem. Biophys. Res. Commun. 200, 1407-1413]. There are no other group IIB or transition metals in the molecule. The zinc atoms are removed instantaneously by 1,10-phenanthroline (OP) (pK 4.8). Once internalized by receptor-mediated endocytosis, vitellogenin is cleaved into multiple polypeptides, i.e., the two lipovitellin subunits (1 and 2) plus phosvitin; these are then stored as microcrystals within yolk platelets. We here show by metal analysis of the individual proteins generated by vitellogenin processing that zinc and calcium occur in different domains of the vitellogenin polypeptide chain. All of the vitellogenin zinc is present in lipovitellin, in amounts equal to 1 mol of zinc/141 kDa. Calcium, in contrast, is detected exclusively in phosvitin which, in addition, contains 3 mol of magnesium/35 kDa, apparently acquired following vitellogenin entry into the oocyte. The zinc in lipovitellin is removed by OP in a concentration-dependent manner with a pK of 4.8, identical to that obtained for vitellogenin, and by exposure to acidic conditions (below pH 5). Following removal of zinc, the two lipovitellin subunits remain associated, suggesting that zinc is not involved in their interaction. On exposure to 1% SDS, lipovitellin does dissociate into 106 and 33 kDa subunits. The presence of stoichiometric quantities of zinc in both vitellogenin and lipovitellin calls for the study of the hitherto unrecognized biochemistry and functions of these proteins in zinc metabolism and development of the frog oocyte and embryo.

Asunto(s)

Proteínas Dietéticas del Huevo/química , Metaloproteínas/química , Vitelogeninas/química , Zinc/análisis , Secuencia de Aminoácidos , Aminoácidos/análisis , Animales , Sitios de Unión , Calcio/análisis , Calcio/metabolismo , Quelantes/farmacología , Proteínas del Huevo , Proteínas Dietéticas del Huevo/aislamiento & purificación , Proteínas Dietéticas del Huevo/metabolismo , Electroforesis en Gel de Poliacrilamida , Endocitosis , Concentración de Iones de Hidrógeno , Magnesio/análisis , Datos de Secuencia Molecular , Oocitos/química , Fenantrolinas/farmacología , Fosvitina/química , Desnaturalización Proteica , Análisis de Secuencia , Dodecil Sulfato de Sodio/farmacología , Vitelogeninas/sangre , Vitelogeninas/aislamiento & purificación , Vitelogeninas/metabolismo , Xenopus laevis , Zinc/metabolismo

RESUMEN

Frog oocytes and embryos have long served as traditional subjects of embryological research providing structural and functional information for the interpretation of the biological processes underlying development. A large number of various chemical agents induce typical teratological changes in frog embryos. However, the effects of metal deficiency of the first transition and IIB series or of chelating agents specific for these metals have never been examined in the frog. Multidentate chelating agents, including 1,10-phenanthroline (OP), which coordinate metals through N, O or S donor atoms are teratogenic also but in a manner characteristic for this class of reagents and completely different from those referred to above. Exposure to 10(-5) M OP causes maximal malformations with minimal mortality inducing craniofacial and skeletal abnormalities with failure of eye, head and other organ formation in 74% of frog embryos. In contrast, the non chelating analogue 1,7-phenanthroline (MP) has no effect at this concentration. A concentration of 10(-3) M OP is lethal. The known characteristics of either zinc and/or iron complexes with OP as well as the concentrations of these elements in frog oocytes and embryos are consistent with the hypothesis that the teratological observations are due to an effect of OP on either zinc or iron proteins.

Asunto(s)

Anomalías Inducidas por Medicamentos , Fenantrolinas/toxicidad , Teratógenos , Xenopus laevis/embriología , Animales , Quelantes/toxicidad , Metales/metabolismo

RESUMEN

Vitellogenin induced by estrogen administration has been purified from the serum of Xenopus laevis. Six days after hormone injection, serum was collected and treated with 35% sat. (NH4)2SO4 to remove globulins. A single vitellogenin containing fraction was isolated by chromatography on a Mono-Q column. The protein was identified on the basis of its amino acid composition. N-terminal sequence analysis and SDS-PAGE demonstrated the presence of two forms of vitellogenin (A and B). Consistent with the conclusions drawn from the teratology which chelating agents induce in frog embryos (1), metal analysis shows that vitellogenin is a zinc protein with 1 g atom zinc/220 kDa monomer but containing no other IIB and transition metals.

Asunto(s)

Metaloproteínas/química , Vitelogeninas/química , Secuencia de Aminoácidos , Aminoácidos/análisis , Animales , Datos de Secuencia Molecular , Xenopus laevis

RESUMEN

Zinc is essential for vertebrate development; its deficiency results in multiple congenital malformations. Knowledge of the zinc biochemistry that underlies embryologic development is very limited. This has led us to investigate the zinc, iron, and copper contents of Xenopus laevis oocytes and embryos. Stage 1-6 oocytes, isolated from ovaries, and stage 1-40 embryos, obtained by in vitro fertilization techniques, were washed in metal-free water prior to digestion by 70% ultrapure HNO3. The metal content of the digests was analyzed by atomic absorption spectrometry. Stage 6 oocytes contain 65.8 +/- 4, 31.1 +/- 3, and 0.68 +/- 0.2 ng of zinc, iron and copper, respectively. The corresponding concentrations are 1, 0.5, and 0.01 mM in 1 microliter eggs. The metal content varies as a function of egg maturation. The zinc content increases from 3-7 to > 60 ng by stages 3 and 6, respectively. A similar pattern is noted for iron, which increases from 2-5 to 30 ng at analogous stages. In contrast, the copper content remains virtually unchanged in oocytes undergoing maturation. Importantly, the total of all three metals does not vary throughout the first 50 stages of development, when all tadpole organs are forming. Hence, the full complement of zinc, iron, and copper needed for incorporation into apoproteins during development is already present at a time when oocyte maturation is completed. The specific metalloproteins that store, donate, and accept these metals during induction and organogenesis and the alterations caused by metal deficiency can now be identified.

Asunto(s)

Cobre/metabolismo , Hierro/metabolismo , Oocitos/metabolismo , Zinc/metabolismo , Animales , Embrión no Mamífero/metabolismo , Desarrollo Embrionario y Fetal , Femenino , Oocitos/crecimiento & desarrollo , Espectrofotometría Atómica , Factores de Tiempo , Xenopus laevis

RESUMEN

A 26-kDa endonuclease has been purified to homogeneity from zinc-sufficient Euglena gracilis. The protein binds to single-stranded DNA with a higher affinity than to double-stranded DNA, but it exhibits nucleolytic activity toward both. Thus, it converts supercoiled plasmid pBR322 DNA into the linear form, a property characteristic of endonucleases, and it continues to act on the linearized DNA until it is completely degraded. It also hydrolyzes heat-denatured, single-stranded calf thymus DNA. Moreover, at amounts below 1 microgram, it enhances RNA synthesis by RNA polymerase II, a characteristic observed with other DNases. Its addition to an in vitro transcription assay increases RNA synthesis up to 3-fold. The nuclease requires two metal components to carry out its enzymatic activities. It hydrolyzes DNA only in the presence of millimolar amounts of magnesium or micromolar quantities of other activating metal ions, such as manganese, zinc, or cobalt. However, even when optimal concentrations of Mg2+ are present, micromolar amounts of the metal-chelating agents OP and HQSA completely inhibit pBR322 digestion. Transcription enhancement is also inhibited completely by both chelators at concentrations that do not affect the intrinsic polymerase II activity. By atomic absorption spectrometry, the enzyme contains 1 g-atom of Zn/mol, which is the likely target of chelator action. The nuclease protein can also be isolated from zinc-deficient E. gracilis, but remarkably it then contains 1 mol of Cu/g-atom and no zinc.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Endodesoxirribonucleasas/metabolismo , Euglena gracilis/enzimología , Metaloproteínas/metabolismo , Zinc/análisis , Aminoácidos/análisis , Animales , Quelantes/farmacología , Cromatografía de Afinidad , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Endodesoxirribonucleasas/aislamiento & purificación , Cinética , Metaloproteínas/genética , Metaloproteínas/aislamiento & purificación , Metales/análisis , Fenantrolinas/farmacología , ARN Polimerasa II/metabolismo , Espectrofotometría Atómica , Transcripción Genética/efectos de los fármacos

Asunto(s)

Zinc/fisiología , Animales , Artritis Reumatoide/patología , Sistema Digestivo/patología , Fenómenos Fisiológicos del Sistema Digestivo , Glándulas Endocrinas/patología , Glándulas Endocrinas/fisiología , Humanos , Sistema Inmunológico/fisiología , Neoplasias/patología , Sistema Nervioso/patología , Fenómenos Fisiológicos del Sistema Nervioso , Fenómenos Fisiológicos Oculares , Reproducción/fisiología , Piel/patología , Zinc/efectos adversos , Zinc/química , Zinc/deficiencia

Asunto(s)

Transcripción Genética , Zinc/fisiología , Animales , Diferenciación Celular , División Celular , Replicación del ADN , Humanos , Morfogénesis , Factores de Transcripción/fisiología , Zinc/deficiencia

Asunto(s)

Metalotioneína/química , Oligoelementos/análisis , Indicadores y Reactivos , Estándares de Referencia , Solventes , Espectrofotometría Atómica/métodos

Asunto(s)

Metalotioneína/aislamiento & purificación , Animales , Tampones (Química) , Quelantes , Cromatografía Líquida de Alta Presión/métodos , Cromatografía por Intercambio Iónico/métodos , Contaminación de Equipos , Vidrio , Indicadores y Reactivos , Metales , Plantas

Asunto(s)

Zinc/análisis , Animales , Enzimas/análisis , Humanos , Indicadores y Reactivos , Microquímica , Espectrofotometría Atómica/métodos , Zinc/sangre , Zinc/orina

RESUMEN

Iron-, manganese-, or magnesium-deficiency has been induced in Euglena gracilis. Each arrests cell proliferation, decreases the intracellular content of the deficient metal, and increases that of several other metals. Light and electron microscopy of stationary phase cells reveal that Fe-deficient (-Fe) cells are similar in size and shape to control organisms. Magnesium-deficient (-Mg) cells, however, are larger, and approximately 14% are multilobed, containing 2 to 12 lobes of equal size emanating from a central region. Individual (-Mg) cells and each lobe of multilobed cells contain a single nucleus. Manganese-deficient (-Mn) organisms are morphologically more heterogeneous than (-Fe) or (-Mg) cells. Most are spherical and larger than controls. Approximately 15% are multilobed but, unlike (-Mg) cells, contain lobes of unequal size with either zero, one, or several nuclei present in each. Nuclei of (-Mn) cells differ in size and shape from those of control, (-Fe), or (-Mg) cells. All three deficient cell types accumulate large quantities of paramylon. Other cytoplasmic structures, however, appear normal. Addition of Fe, Mn, or Mg to the respective deficient stationary phase cultures reverses growth arrest and restores normal morphology. The results suggest that Fe-, Mn-, and Mg-deficiencies affect different stages of the E. gracilis cell cycle.

Asunto(s)

Euglena gracilis/crecimiento & desarrollo , Hierro/metabolismo , Magnesio/metabolismo , Manganeso/metabolismo , Núcleo Celular/ultraestructura , Medios de Cultivo , Gránulos Citoplasmáticos/ultraestructura , Euglena gracilis/análisis , Euglena gracilis/citología , Euglena gracilis/ultraestructura , Hierro/análisis , Magnesio/análisis , Manganeso/análisis , Metales/análisis , Microscopía Electrónica , Organoides/ultraestructura

RESUMEN

The effects induced by Fe, Mn, or Mg deficiency or cold shock on the DNA content and histones of Euglena gracilis have been examined and compared to those produced by Zn deficiency. The DNA content of the stationary-phase organisms used as controls is 2.1 micrograms/10(6) cells. The DNA of stationary-phase iron-deficient (-Fe), magnesium-deficient (-Mg), manganese-deficient (-Mn), zinc-deficient (-Zn), and cold-shocked (CS) cells is increased to 3.0, 4.6, 6.2, 3.8, and 3.8 micrograms/10(6) cells, respectively. The electrophoretic mobilities of proteins solubilized with 0.4 N H2SO4 from CS, -Fe, -Mg, and -Mn cells are nearly identical and are characteristic of the five histone classes, H1, H2A, H2B, H3, and H4. In contrast, no histones are found in the equivalent acid extract from -Zn cells. The effect of micrococcal nuclease on chromatin from control, CS, and -Zn cells was examined. The chromatin of CS cells is 1.2-fold while that from -Zn cells is 10-30-fold more resistant to micrococcal nuclease digestion than is the chromatin of control cells. Thus, the chromatin of cells grown in Zn-deficient conditions differs markedly from that of organisms cultured in media deficient in Fe, Mn, or Mg or exposed to cold shock.

Asunto(s)

Aclimatación , Cromatina/fisiología , Euglena gracilis/crecimiento & desarrollo , Hierro/farmacología , Magnesio/farmacología , Manganeso/farmacología , Zinc/farmacología , Animales , Cromatina/efectos de los fármacos , Frío , ADN/análisis , Euglena gracilis/efectos de los fármacos , Euglena gracilis/fisiología , Histonas/metabolismo

RESUMEN

Copper complexes of 1,10-phenanthroline (OP-Cu) hydrolyze DNA [D'Aurora, V., Stern, A. M., & Sigman, D. S. (1978) Biochem. Biophys. Res. Commun. 80, 1025-1032; Marshall Pope, L., Reich, K. A., Graham, D. R., & Sigman, D. S. (1982) J. Biol. Chem. 257, 12121-12128]. This reaction has been studied to determine whether the 1,10-phenanthroline (OP) inhibition of the activity of RNA and DNA polymerases is the result of template hydrolysis or the chelation of a metal associated with and essential to the function of these enzymes. Addition of 4',6-diamino-2-phenylindole dihydrochloride (DAPI) to DNA generates a fluorescence signal with a linear increase of the intensity over a broad range of DNA concentrations from 0 to 100 micrograms/mL. The progress of hydrolysis of DNA by DNase I or OP (2 mM) is monitored by the time-dependent decrease in DAPI-induced fluorescence. In the presence of OP, the rate of hydrolysis increases as the Cu2+ concentration in the reaction mixture rises from 10(-8) to 10(-5) M. The rate differs for each nucleic acid template used; hydrolysis of poly(dA-dT) greater than denatured DNA greater than double-stranded DNA. However, millimolar amounts of OP do not hydrolyze the template even in the presence of Cu2+ (10(-6) M) when DNA is complexed with either Escherichia coli DNA polymerase I or Euglena gracilis or wheat germ RNA polymerase II. Under the same conditions, OP inhibits the activity of both varieties of RNA polymerase II with pKi's of 3.4 and 3.0, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Asunto(s)

Quelantes/farmacología , Euglena gracilis/enzimología , Fenantrolinas/farmacología , Plantas/enzimología , ARN Polimerasa II/antagonistas & inhibidores , Zinc/análisis , Cobre/farmacología , Cinética , Relación Estructura-Actividad , Especificidad por Sustrato , Triticum/enzimología