RESUMEN
The aim of this study was to investigate the effect of probiotic strains of Lactobacillus casei IMV B-7280, Bifidobacterium animalis VKL, B. animalis VKB on the pro- and anti-inflammatory cytokines production in Wistar male rats with monosodium glutamate (MSG)-induced obesity. It was established that neonatal administration of MSG to rats leads to increasing levels of the interleukin (IL)-1ß and IL-12, and to decreasing ofthe IL-4, IL-10 and tumor growthfactor (TGF)-ß levels in the bloodserum. After administration of the B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition to obese rats the level of the IL-lP in blood serum wasn't differ from that in the obese rats, that didn't receive of the probiotic bacteria. But there was no statistically signifcant difference comparing with intact rats. The level of the IL-12B p4O in blood serum was decreased under influence of the B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition (18.9%, p < 0.05) and B. animalis VKL (10.5%, p < 0.05) compared with obese rats, not receiving probiotic bacteria, but remained higher than in intact animals. After administration to obese rats ofthe B. animalis VKL - B. animalis VKB - L. casei IMV B-7280 composition the levels ofthe IL-4, IL- 10 and TGF-ß increased in blood serum comparing with obese rats, not receiving probiotic bacteria. The level of the IL-10 also increased under influence of the B. animalis VKB, and IL-4 - under influence of the L. casei IMVB-7280. Our results suggest that these probiotic bacteria and probiotic composition are able to down-regulation the inflammation in rats with MSG-induced obesity but the strongest anti-inflammatory effects have probiotic composition. The ability of lactobacilli and bifdobacteria to alter the pro- and anti-inflammatory cytokines production, opens perspectives to create new treatments for obesity and metabolic syndrome based on probiotics.
Asunto(s)
Antiinflamatorios/administración & dosificación , Bifidobacterium/fisiología , Regulación de la Expresión Génica/efectos de los fármacos , Lacticaseibacillus casei/fisiología , Obesidad/terapia , Probióticos/administración & dosificación , Animales , Animales Recién Nacidos , Regulación de la Expresión Génica/inmunología , Inflamación , Interleucina-10/genética , Interleucina-10/inmunología , Interleucina-12/genética , Interleucina-12/inmunología , Interleucina-1beta/genética , Interleucina-1beta/inmunología , Interleucina-4/genética , Interleucina-4/inmunología , Obesidad/inducido químicamente , Obesidad/inmunología , Obesidad/patología , Ratas , Ratas Wistar , Glutamato de Sodio/administración & dosificación , Glutamato de Sodio/antagonistas & inhibidores , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta/inmunologíaRESUMEN
It was established that with age the content of lipid peroxidation products increased in the mucosa of the stomach: Diene conjugates by 30%, products which react to thiobarbituric acid by 285% and Schif bases by 181%. Nanodisperse cerium dioxide (NCD) reduced the content of lipid peroxidation in the gastric mucosa in old rats: Diene conjugates by 43 %, products which react to thiobarbituric acid by 51% and Schif bases by 44% relative to the control group of rats given age. Similarly, it was established that the content of Diene conjugates increased by 40%, products which react to thiobarbituric acid by 114% and Schif bases by 132% in the mucosa of the colon of old rats. NDC significant reduced the content of products which react to thiobarbituric acid by 69% and Schyf basics by 132%. In the stomach superoxide dismutase (by 43%) and catalase activity (by 24%) decreases with age, while in the colon superoxide dismutase activity increases (by 43%). In the colon NCD significant decreased superoxide dismutase (by 34%) and catalase activity (by 21%) relative to controls. Thus, the NDC restores lipid peroxidation in the gastric mucosa and colon, in which develops oxidative stress with age.
Asunto(s)
Envejecimiento/metabolismo , Antioxidantes/metabolismo , Cerio/farmacología , Mucosa Gástrica/efectos de los fármacos , Mucosa Intestinal/efectos de los fármacos , Nanopartículas/química , Envejecimiento/efectos de los fármacos , Animales , Catalasa/metabolismo , Cerio/química , Mucosa Gástrica/enzimología , Mucosa Intestinal/enzimología , Peroxidación de Lípido/efectos de los fármacos , Peróxidos Lipídicos/metabolismo , Estrés Oxidativo/efectos de los fármacos , Ratas , Superóxido Dismutasa/metabolismoRESUMEN
We studied the influence of melanin from yeast-like fungi Nadsoniella nigra strain X1 on the changes of the levels of adrenocorticotropic hormone (ACTH) and cortisol in blood serum of rats, adrenal glands weight ratio and lesions of the gastric mucosa (GM) caused by neuromuscular tension by Selye. Melanin administration restored functioning of the hypothalamic-pituitary-adrenal axis that was evident by an increase of ACTH concentration by 42% and a decrease of cortisol concentration by 19% compared to the rats injected with water (group 2). In rats treated with melanin, the adrenal glands weight ratio, didn't differ from intact control group of the rats. Melanin decreased ulcers area by 64% and reduced the content of free hydroxyproline by 29%, the free fucose by 16% and the free hexuronic acids by 24% in the GM compared to the group 2 of the rats. It is established that the mechanism of melanin stress-protective properties are based on its regulation of the glucocorticoids secretion and prevention of GM collagen and extracellular matrix substances depolymerization. Melanin possesses gastroprotective properties and is a perspective agent for preventing and treatment of consequences of the stress influence on the organism.
Asunto(s)
Glándulas Suprarrenales/efectos de los fármacos , Mucosa Gástrica/efectos de los fármacos , Sistema Hipotálamo-Hipofisario/efectos de los fármacos , Melaninas/farmacología , Sistema Hipófiso-Suprarrenal/efectos de los fármacos , Sustancias Protectoras/farmacología , Glándulas Suprarrenales/metabolismo , Glándulas Suprarrenales/patología , Hormona Adrenocorticotrópica/sangre , Animales , Hormona Liberadora de Corticotropina/sangre , Femenino , Fucosa/metabolismo , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patología , Ácidos Hexurónicos/metabolismo , Hidrocortisona/sangre , Hidroxiprolina/metabolismo , Sistema Hipotálamo-Hipofisario/metabolismo , Sistema Hipotálamo-Hipofisario/fisiopatología , Sistema Hipófiso-Suprarrenal/metabolismo , Sistema Hipófiso-Suprarrenal/fisiopatología , Ratas , Estrés Fisiológico/efectos de los fármacos , Estrés Psicológico/tratamiento farmacológicoRESUMEN
It was studied the effect of probiotic strains of Bifidobacterium animalis VKL, Bifidobacterium animalis VKB and Lactobacillus casei IMVB-7280, and their mixtures on erosive and ulcerative lesions of the gastric mucosa (GM) of rats. GM was induced by water-immersion restraint stress. It was found that investigated probiotics did not have gastroprotective properties under a single and seven-day prophylactic administration. However, multiprobiotics (polyprobiotic Bifidobacterium animalis VKL and Bifidobacterium animalis VKB and composite probiotic Bifidobacterium animalis VKL, Bifidobacterium animalis VKB and Lactobacillus casei IMVB-7280) reduced the erosive and ulcerative lesions and the intensity of bleeding in rat GM when given within 14 days. It was shown that one of the mechanisms of antiulcer preventive effect of the multistrain probiotics is the restoration of pro/antioxidant balance in the GM under the stress action. The obtained results show the effectiveness of poly- and composite probiotics in the gastric ulcer prevention.
Asunto(s)
Bifidobacterium/fisiología , Hemorragia Gastrointestinal/dietoterapia , Inmovilización/efectos adversos , Lacticaseibacillus casei/fisiología , Probióticos/farmacología , Úlcera Gástrica/dietoterapia , Animales , Animales no Consanguíneos , Femenino , Mucosa Gástrica/irrigación sanguínea , Mucosa Gástrica/efectos de los fármacos , Mucosa Gástrica/patología , Hemorragia Gastrointestinal/etiología , Hemorragia Gastrointestinal/patología , Ratas , Estómago/irrigación sanguínea , Estómago/efectos de los fármacos , Estómago/patología , Úlcera Gástrica/etiología , Úlcera Gástrica/patología , Estrés FisiológicoRESUMEN
The decrease in the severity of erosions and ulcer lesions after preventive treatment with PGP or PG correlated with a decrease in the content of lipid peroxidation products to a control level. Activities of SOD and catalase also returned to control values. GP produced the weakest effect on pro- and antioxidant state of the gastric mucosa. We concluded that the pronounced preventive effect of PGP and PG on the development of ethanol-induced erosions and ulcer lesions is largely determined by their antioxidant properties. Glyprolines can be considered as a promising means for prevention and treatment of stomach and duodenal ulcers.
Asunto(s)
Etanol/toxicidad , Mucosa Gástrica/fisiología , Homeostasis/fisiología , Prolina/fisiología , Úlcera Gástrica/inducido químicamente , Animales , Peroxidación de Lípido , Masculino , Ratas , Ratas Wistar , Úlcera Gástrica/metabolismoRESUMEN
Pro-Gly-Pro and its metabolite Gly-Pro effectively prevented the development of erosive and ulcerative lesions of the gastric mucosa in rats under conditions of water-immersion restraint stress by restoring the oxidatant-antioxidant balance in the total fraction of gastric mucosa cells. Pro-Gly was least effective in this respect. We conclude that glyprolines hold much promise as pharmaceutical products, which can be used in gastroenterological practice for the prevention and therapy of ulcer disease of the stomach and duodenum.