RESUMEN
The neuroprotective action of hybrid structures based on fullerene C60 with attached proline amino acid has been studied. Hybrid structures contained natural antioxidant carnosine or addends with one or two nitrate groups. It has been shown that all studied compounds had antioxidant activity and decreased the concentration of malondialdehyde in homogenates of the rat brain. Compound 1, which contained the antioxidant carnosine, has been found to be the most effective antioxidant. All compounds except IV and V inhibited the activity of monoamine oxidase B, while compounds I-IV increased the activity of monoamine oxidase A. All investigated compounds inhibited glutamate-induced Ca2+ uptake into synaptosomes of the rat brain cortex. Compound III, containing two nitrate groups, has been found to be the most effective inhibitor. This compound caused a significant increase of the currents of AMPA receptors (AMPA, alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid).
Asunto(s)
Antioxidantes/farmacología , Encéfalo/efectos de los fármacos , Fulerenos/farmacología , Fármacos Neuroprotectores/farmacología , Animales , Antioxidantes/química , Encéfalo/citología , Encéfalo/enzimología , Encéfalo/metabolismo , Calcio/metabolismo , Fulerenos/química , Técnicas In Vitro , Peroxidación de Lípido/efectos de los fármacos , Malondialdehído/metabolismo , Mitocondrias/efectos de los fármacos , Mitocondrias/enzimología , Estructura Molecular , Monoaminooxidasa/metabolismo , Fármacos Neuroprotectores/química , Ratas , Receptores AMPA/metabolismo , Sinaptosomas/efectos de los fármacos , Sinaptosomas/metabolismoRESUMEN
The action of heterocyclic amides series (ethosuximide, phenytoin, primidone) on lipid peroxidation and membrane bound monoamine oxidases A and B under stress condition has been studied. The intraperitoneal injection of the drugs resulted in enhancement of SOD, decrease of brain malondialdehyde content and mitochondrial activity of monoamine oxidases A and B.