RESUMEN

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

Asunto(s)

Enzimas/metabolismo , Acetilcoenzima A/química , Adenosina Difosfato/química , Adenosina Trifosfato/química , Coenzima A/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción